Diagnosis of Q Fever and Brucellosis in Aborted Ovine Fetuses by Microbiological, Pathological and Immunohistochemical Methods

Yesilmen Simten 1 , Yaman Turan 2 , Sağsöz Hakan 3 , and Bademkiran Servet 4
  • 1 Department of Microbiology, Faculty of Veterinary Medicine, University of Dicle, Diyarbakir, Turkey
  • 2 Department of Pathology, Faculty of Veterinary Medicine, University of Van Yuzuncu Yil, Van, Turkey
  • 3 Department of Histology and Embryology, Faculty of Veterinary Medicine, University of Dicle, Diyarbakir, Turkey
  • 4 Department of Obstetric and Reproduction, Faculty of Veterinary Medicine, , Diyarbakir, Turkey

Abstract

Brucellosis and Q fever, two zoonoses, are important causes of abortion in ruminants, as well as economically significant diseases caused by a gram-negative bacterium. Determination of these diseases is therefore of great importance. In this study, the organs of 35 naturally infected and aborted ovine fetuses were examined for the presence of changes resulting from infections by Brucella melitensis and Coxiella burnetii, according to macroscopic, bacteriological, histopathological and immunohistochemical methods. B. melitensis was observed in 21 cases, and C. burnetii was observed in 8 cases of the aborted ovine fetuses, and these were determined with immunohistochemical methods. Brucellosis was observed in 18 of the aborted ovine fetuses, and this was determined by microbiological methods. Negative (-) results were found for all of the other fetuses. The Brucella antigen was determined to be localized as intracytoplasmic in mainly alveolar macrophages, bronchi, bronchioles, glandular epithelial cells around bronchial glands, neutrophils, hepatocytes and Kupffer cells. The Coxiella antigen was found to be localized in the alveolar macrophages in the lungs, bronchi, bronchioles and alveolus, and in the cytoplasms of bronchial gland epithelial cells, and in the cytoplasms of hepatocytes and Kupffer cells in the liver. Immunohistochemical and microbiological diagnoses of brucellosis and coxiellosis were compared; it was concluded that immunohistochemical methods were more safely applied than microbiological methods.

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