The aim of this study was to assess whether atipamezole can restrain telazol/xylazine induced expression of c-fos in the rat brain. Rats were injected with a mixture of 13.81 mg/kg telazol and 5.21 mg/kg xylazine, following 10 min later 0.522 mg/kg atipamezole. Thereon, the thalamencephal and cerebral cortex were removed one hour after the last injection. The level of Fos protein was measured in the brain tissue by Westernblot. The results revealed that atipamezole attenuates telazol/xylazine induction of c-fos expression in the thalamencephal and cerebral cortex. The results indicated that atipamezole is able to inhibit telazol/xylazine-induced c-fos expression in the rat brain, thus protecting it from nerve damage.
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1. Kiyoshige T Takashi S Mitsuhiko M: The comparison of effects of various anesthetics on expression of Fos protein in the rat brain. Neuroscience Letter 1994 176(1): 59-62.
2. Mitcheltree DH Serfass WM Tzilkowski RL Peper MT Whary RP: Physiological responses of fishers to immobilization with ketamine ketamine-xylazine or Telazol. Wildlife Society Bulletin 1999 27(3): 582-591.
3. Golden HN Shults BS Kunkel KE: Immobilization of wolverines with Telazol from a helicopter. Wildlife Society Bulletin 2002 30(2): 492-497.
4. Janovsky M Tataruch F Ambuehl M Giacometti M: A Zoletil®-Rompun® mixture as an alternative to the use of opioids for immobilization of feral red deer. Journal of Wildlife Diseases 2000 36(4): 663-669.
5. Hellyer P Muir WW Hubbell JA Sally J: Cardio respiratory effects of the intravenous administration of tiletamine-zolazepam to cats. Veterinary Surgery 1988 17(7): 105-110.
6. Jang HS Kwon YS Lee MG Jang KH: The effect of tiletamine/zolazepam combination with xylazine or medetomidine on electroencephalograms in dogs. Journal of Veterinary Medical Science 2004 66(5): 501-507.
7. Natalini CC Alves SD Guedes AG Polydoro AS Brondani JT Bopp S: Epidural administration of tiletamine/zolazepam in horses. Veterinary Anaesthesia and Analgesia 2004 31(4): 79-85.
8. Lu DZ Fan HG Ma K Jiang S Wang HB: Effect of Addition of Tramadol to Tiletamine/ zolazepam-Xylazine Anaesthesia in Miniature Pigs. Veterinary Record 2010 167(13): 489-492.
9. Miller BF Muller LI Doherty T Osborn DA Miller KV Warren RJ: Effectiveness of antagonists for tiletamine-zolazepam/xylazine immobilization in female white-tailed deer. Journal of Wildlife Diseases 2004 40(3): 533-537.
10. Merwin DS Milspaugh JJ Brundige GC Schultz D Tyner CL: Immobilization of freeranging Rocky Mountain bighorn sheep Ovis canadensis canadensis ewes with Telazol® and xylazine hydrochloride. Canadian Field-Naturalist 2000 114(3): 471 -475.
11. Cattet MR Caulkett NA Stenhouse GB: The comparative effects of chemical immobilizing drug and method of capture on the health of grizzly bears. Thirteenth International Conference on Bear Research and Management Jackson Wyoming 2001 5: 20-26.
12. Belant JL: Belant Field Immobilization of Raccoons (Procyon lotor) with Telazol and Xylazine. Journal of Wildlife Disease 2004 40(4): 787-790.
13. Belant JL: Tiletamine-zolazepam-xylazine immobilization of American marten (Martes Americana). Journal of Wildlife Disease 2005 41(3): 659-663.
14. Belant JL: Tiletamine-zolazepam-xylazine Immobilization of Fishers (Martes pennanti). Journal of Wildlife Disease 2007 43(2): 279-285.
15. Jalanka HH Roeken BO: The use of medetomidine-ketamine combinations and atipamezole in nondomestic mammals: a review. Journal of Zoo & Wildlife Medicine 1990 21(3): 259-282.
16. Dematteis A Menzano A Canavese G Meneguz PG Rossi L: Anaesthesia of freeranging Northern chamois (Rupicapra rupicapra) with xylazine/ketamine and reversal with atipamezole. European Journal of Wildlife Research 2009 55(6): 567 -573.
17. Jung SY Kim MS Lee NY Kim SY Seo KM Nam TC: The reverse effects of atipamezole on medetomidine-tiletamine/zolazepam combination anesthesia in the green iguana (Iguana iguana). Journal of Veterinary clinics 2006 23(1): 18-21.
18. Heaton-Jones TG Ko JC Heaton-Jones DL: Evaluation of medetomidine-ketamine anesthesia with atipamezole reversal in American alligators (Alligator Mississippi -ensis). Journal of Zoo & Wildlife Medicine 2002 33(1): 36-44.
19. Ezgi T Lutfiye K Ayfer Y: Effect of γ-glutamylcysteine ethylester on the levels of c-fos mRNA expression glutathione and reactive oxygen species formation in kainic acid excitotoxicity. Journal of Pharmacy and Pharmacology 2010 62(8): 1010-1017.
20. Sharp FR Sagar SM: Alterationa in gene expression as an index of neuronal injury: heat shock and the immediate early gene response. Neurotoxicology 1994 15(1): 51-59.
21. Dragunow M Faull R: The use of c-fos as metabolic marker in neuronal pathway tracing. Journal of Neuroscience Methods 1989 29(3): 261-265.
22. Griffiths R Malcolm C Ritchie L Frandsen A Schousboe A Scott M Rumsby P Meredith C: Association of c-fos mRNA expression and excitotoxicity in primary cultures of mouse neocortical and cerebellar neurons. Journal of Neuroscience Research 1997 48(6): 533-542.
23. Willoughby JO Mackenzie L Medvedev A Hiscock JJ: Fos induction following systemic kainic acid: early expression in hippocampus and later widespread expression correlated with seizure. Neurosience 1997 77(2): 379-392.
24. Niles LP Smith LJ Tenn CC: Modulation of c-fos expression in the rat striatum by diazepam. Neuroscience Letters 1997 236(1): 5-8.
25. Kumar A Mann HJ Remmel RP: Determination of constituents of Telazol® -tiletamine and zolazepam by a gas chromatography/mass spectrometry-based method. Journal of Chromatography B 2006 842(2): 131-135.
26. Lee CC Lin YY Hsu CW Chu SJ Tsai SH: Movement disorder caused by abuse of veterinary anesthesia containing tiletamine. American Journal of Emergency Medicine 2009 27(8): 1022.e5-1022.e6.
27. Nieradko B Borzecki A: Effect of bifenthrin on memory processes movement activity and coordination in mice exposed to transient cerebral oligaemia. Bulletin of Veterinary Institute in Pulawy 2008 52(2): 291-294.
28. Yin BS Wang HB Gong DQ Li GJ Gao L: Effects of Xylazine on glutamate and GABA contents in the hippocampus and thalamencephal in the rat. Bulletin of Veterinary Institute in Pulawy 2011 55(3): 537-539.
29. Lipták T Capík I Ledecký V Nagy O Kuricová M Tóthová C Mad Aďari A Farbáková J Petrovič V Horňák S: Cardiorespiratory and hemodynamic effects of medetomidine or xylazine with atropine and diazepam premedication for total intravenous anesthesia induced and maintained with propofol/fentanyl in dogs undergoing surgery. Acta Veterinaria- Beograd 2014 64 (4): 466-476.
30. Krukoff TL Morton TL Harris KH Jhamandas JH: Expression of c-fos protein in rat brain elicited by electrical stimulation of the pontine parabrachial nucleus. Journal of Neuroscience 1992 12(9): 3582-3590.
31. Shimode N Fukuoka T Tanimoto M Tashiro C Tokunaga A Noguchi K: The effects of dexmedetomidine and halothane on Fos expression in the spinal dorsal horn using a rat postoperative pain model. Neuroscience Letter 2003 343(1): 45-48.
32. Miller J: induction of c-fos mRNA expression in rat striatum by neuroleptic drugs. Neurochemistry 1990 54(4): 1453-1455.
33. Pertovaara A Haapalinna A Sirviö J Virtanen R: Pharmacological Properties Central Nervous System Effects and Potential Therapeutic Applications of Atipamezole a Selective α2-Adrenoceptor Antagonist. CNS Drug Reviews 2005 11(3): 273-288.
34. Ruth LS Michele CG Patrice GG: Atipamezole-precipitated clonidine withdrawal induces c-Fos expression in rat central nervous system. Brain Research 1997 764(1-2): 81-92.