Campylobacteriosis is an infectious human disease caused by thermophilic Campylobacter species, mainly C. jejuni and C. coli. It is the leading cause of human gastroenteritis today with the number of cases surpassing the number of Salmonella poisoning cases. The epidemiology of the agent is not completely clear, but a number of investigations indicate an important role of broiler meat in human infections. The purpose of this study was to investigate the diversity of Campylobacter jejuni strains present in the Republic of Serbia and to determine a fast and reliable system for the confirmation and typing of the isolated strains. Samples taken at slaughterhouses, broiler farms, as well as two human isolates of Campylobacter species have been investigated. Strain identification was performed by multiplex-PCR. Genotyping was performed by Random Amplified Polymorphic DNA (RAPD) with multiple primers. Using several unusual primers and a newly designed one (DJP17), we report on the RAPD types of indigenous Campylobacter species. RAPD profiles showed different levels of discrimination between the isolates, depending on the primer: SPH1 and AG15 were informative only in part and better results were obtained with AP10, AK16 and DJP17.
4. European Food Safety Authority: Scientifi c Opinion on Campylobacter in broiler meat production: control options and performance objectives and/or targets at different stages of the food chain. EFSA Journal 2011 9:1-141.
5. Scallan E Hoekstra RM Angulo FJ Tauxe RV Widdowson MA Roy SL Jones JL Griffin PM: Foodborne Illness Acquired in the United States-Major Pathogens. Emerg Infect Dis 2011 17:7-15.
6. Devane ML Nicol C Ball A Klena JD Scholes P Hudson JA Baker MG Gilpin BJ Garrett N Savil MG: The occurrence of Campylobacter subtypes in environmental reservoirs and potential transmission routes. J Appl Microbiol 2005 98:980-990.
7. Stanley K Jones K: Cattle and sheep farms as reservoirs of Campylobacter. J Appl Microbiol 2003 94:104S-113S.
8. OIE Terestrial manual: Campylobacter jejuni and Campylobacter coli. 2008
9. Achen M Morishita TY Ley EC: Shedding and colonisation of Campylobacter jejuni in broilers from day-of-hatch to slaughter age. Avian Dis 1998 42:732-737.
10. Engberg J Aarestrup FM Taylor DE Gerner-Smidt P Nachamkin I: Quinolone and macrolide resistance in Campylobacter jejuni and Campylobacter coli: resistence mechanisms and trends in human isolates. Emerg Infect Dis 2001 7:24-34.
11. Nadeau R Messier S Quessy S: Prevalence and comparison of genetic profiles of Campylobacter strains isolated from poultry and sporadic cases of campylobacteriosis in humans. J Food Prot 2002 65:73-78.
12. Açik MN Çetinkaya B: Random amplified polymorphic DNA analysis of Campylobacter jejuni and Campylobacter coli isolated from healthy cattle and sheep. J Med Microbiol 2006 55:331-334.
13. Behringer M Miller WG Oyarzabal O: Typing of Campylobacter jejuni and Campylobacter coli isolated from live broilers and retail broiler meat by fl aA-RFLP MLST PFGE and REP-PCR. J Microbiol Meth 2011 84:194-201.
14. Denis M Soumet C Rivoal K Ermel G Blivet D Salvat G Colin P: Development of a m-PCR assay for simultaneous identification of Campylobacter jejuni and Campylobacter coli. Lett Appl Microbiol 1999 29:406-410.
15. Møller Nielsen E Engberg J Fussing V Petersen L Brogren CH On SLW: Evaluation of phenotypic and genotypic methods for subtyping of Campylobacter jejuni isolates from human poultry and cattle. J Clin Microbiol 2000 38:3800-3810.
16. Trajkovska-Dokić E Stojkovska S Icev K Grozdanova A: Serogrouping and randomly amplified polimorphic DNA fingerprinting of Campylobacter jejuni. Maced J Med Sci 2011 4:372-375.
17. Tan YF Haresh KK Chai LC Son R: Antibiotic susceptibility and genotyping by RAPD of Campylobacter jejuni isolated from retailed ready-to-eat sushi. Internat Food Res J 2009 16:31-38.
18. Mliki A Staub AE Zhangyong S Ghorbel A: Genetic diversity in melon (Cucumis melo L.): Anevaluation of African germplasm. Gen Res Crop Evol 2001 48:587-597.
19. Dooley JJ Harrison SP Mytton LR Dye M Cresswell A Skot L Beeching JR: Phylogenetic grouping and identification of Rhizobium isolates on the basis of random amplified polomorphic DNA profiles. Can J Microbiol 1993 39:665-673.
20. Selenska-Pobell S Evguenieva-Hackenberg E Radeva G Squartini A: Characterization of Rhizobium hedusari by RFLP analysis of PCR amplified rDNA and by genomic fingerprinting. J Appl Bacteriol 1996 80:517-528.
21. Al Amri A Senok AC Ismaeel AY Al-Mahmeed AE Botta GA: Multiplex PCR for direct identification of Campylobacter spp. in human and chicken stools. J Med Microbiol 2007 56:1350-1355.
22. Linton D Lawson AJ Owen RJ Stanley J: PCR Detection Identification to Species Level and Fingerprinting of Campylobacter jejuni and Campylobacter coli Direct from Diarrheic Samples. J Clin Microbiol 1997 35:2568-2572.
23. Sinha S Prasad KN Pradhan S Jain D Jha S: Detection of preceding Campylobacter jejuni infection by polymerase chain reaction in patients with Guillain-Barre syndrome. Trans R Soc Trop Med Hyg 2004 98:342-346.
24. Boes J Nersting L Nielsen EM Kranker S Enøe C Wachmann HC Baggesen DL: Prevalence and diversity of Campylobacter jejuni in pig herds on farms with and without cattle or poultry. J Food Prot 2005 68:722-727.
25. Giacomelli M Andrighetto C Rossi F Lombardi A Rizzotti L Martini M Piccirillo A: Molecular characterization and genotypic antimicrobial resistance analysis of Campylobacter jejuni and Campylobacter coli isolated from broiler flocks in northern Italy. Avian Pathol 2012 41:579-588.
26. Stojanov I Stojanović D Milić N Živkov Baloš M Kapetanov M Ašanin N Čogurić I: Influence of salmonella infection in chickens on the outcome of campylobacteriosis in experimental conditions. Acta Veterinaria 2011 61:157-65.