Effects Of Rose-Hip And Grapeseed Dietary Supplementation On Serum Oxidative Stress Parameters In Dogs Before And After Physical Exercise

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Abstract

The aim of this study was to evaluate the effects of 60 days of rose-hip and grapeseed dietary supplementation of a balanced home-cooked diet on serum oxidative stress parameters: ROMs, MDA and FRAP in army service dogs before and after regular physical exercise. The dogs were fed a balanced cooked diet as instructed by army standards until the initial blood sampling in June. Thereon the dogs were randomly allotted to 4 groups according to the dietary regime: dogs maintained on a balanced cooked diet according to army standards, branded dry dog food, cooked diet with added 500 mg rose-hip extract, and cooked diet with added 100 mg grapeseed extract for a 60 day period from June to September after which all 4 groups were fed the standard cooked meal diet. Sampling was performed at the beginning of the experiment (June), 60 days from the start of the treatment (September) and finally 60 days after the end of supplementation (November). Statistical analysis of the results included descriptive statistical parameters: mean (M), standard deviation (SD), and variation coefficient (CV%). In order to test the statistical significance of the differences between treatments a multifactor variance test (ANOVA) was performed for ROM, MDA and FRAP and the combined effects of diet, exercise and time period were observed. The initial (in June) increase in MDA and ROMs after exercise indicates the presence of oxidative stress 30 minutes after exercise. However, the antioxidative effects of rose-hip and grapeseed extracts are not conclusive, as multifactor ANOVA testing of time, diet, and exercise factors did not reveal for MDA statistically significant differences either at 60 days of supplementation nor 60 days after withdrawal of the supplements. Only one distinct exception was recorded for the prolonged antioxidative effects reflected in significantly decreased (p<0.01) ROMs before and after exercise in dogs fed the branded dry food 60 days after the end of such dietary regime (November). FRAP values tend to be higher (p>0.05) after exercise compared to before exercise in all experimental groups in November, indicating on a possible redistribution and upregulation of endogenous antioxidants during the experiment.

1. Mc Cord J M:. Superoxide, superoxide dismutase and oxygen toxicity. Rev. Biochem Toxicol 1979, 10:109–121.

2. Alessio HM, Hagerman AE, Fulkerson BK, Ambrose J, Rice RE, Wiley RL: Lipid and protein oxidation after exhaustive aerobic and isometric exercise. Med Sci Sports Exerc 2000, 32:1576-1581.

3. Watson TA, Mackdonald-Wicks L K, Garg ML: Oxidative stress and antioxidants in athlets undertaking regular exercise training. Int J Sport Nutr Exe 2005, 15:131.

4. Ji LL: Antioxidant signalling in skeletal muscle. A brief review. Exp Geront 2007, 42:582–593.

5. Alberti A, Bolognini L, Macciantrelli D, Carratelli M: The radical cation of N,N-diethyl-para-phenyldiamine: a possible indicator of oxidative stress in biological samples. Research on Chemical Intermediates 2000, 26:253–267.

6. Witt EH, Reznick AZ, Viguie CA, Starke-Reed P, Packer L: Exercise, oxidative damage and effects of antioxidant manipulation. J Nutr 1992, 122:766–773.

7. Kanter MM, Lesmes GR, Kaminsky L A, La Ham-Saefer J, Nequin ND: Serum creatine kinase and lactate dehydrogenase changes following an eighty kilometer race. Relationship to lipid peroxidation. EU J Appl Physiol Occup Physiol 1998, 57:60–63.

8. Ilhan N, Kamanli A, Ozmerdivenli R, Ilhan N: Variable Effects of Exercise Intensity on Reduced Glutathione, Thiobarbituric Acid Reactive Substance Levels, and Glucose Concentration. Arch Med Res 2004, 35:294–300.

9. Benzie IF, Strain JJ: The ferric reducing ability of plasma (FRAP) as a measure of „antioxidant power“: the FRAP assay. Anal Biochem 1996, 239:70–76.

10. Pasquini A, Luchetti E, Cardini G: Evaluation of oxidative stress in hunting dogs during exercise. Res Vet Sci 2010, 89:120–123.

11. Castilla P, Davalos A, Teruel J, Cerrato F, Fernandez-Lucas M, Merino J: Comparative effects of dietary supplementation with red grape juice and vitamin E on production of superoxide by circulating neutrophil NADPH oxidase in hemodialysis patients. Clin Nutr 2008, 87:1053–1061.

12. Belviranh M, Gökbel H, Nilsel O, Basarah K: Effects of grape seed extract supplementation on exercise-induced oxidative stress in rats. Br J Nutr 2012, 108:249-256.

13. Gao X, Bjork L, Trajkovski L, Uggla M: Evaluation of antioxidant activities of rosehip ethanol extracts in different test systems. J Sci Food Agric 2000, 80:2021-2027.

14. Widén C, Ekholm A, Renvert S, Rumpunen K:. Erythrocyte Antioxidant Protection of Rose Hips (Rosa spp.). Oxidative Medicine and Cellular Longevity, 2012, doi:10.1155/2012/621579, Hindawi Publishing Corporation, 8 pages.

15. Kay CD, Holub BJ: The effect of wild blueberry (Vaccinium angustifolium) consumption on postprandial serum antioxidant status in human subjects. Br J Nutr 2002, 88:389–398.

16. Dunlap KL, Reynolds AJ, Duffy LK: Total antioxidant power in sled dogs supplemented with blueberries and the comparison of blood parameters associated with exercise. Comp Biochem Physiol A 2006, 143:429-434.

17. Moskaug JØ, Carlsen H, Myhrstad MC, Blomhoff R: Polyphenols and glutathione synthesis regulation. Am J Clin Nutr 2005, 81 (1 Suppl): 277S–283S.

18. Elfalleh W, Tlili N, Nasri N, Yahia Y, Hannachi H, Chaira N: Antioxidant capacities of phenolic compounds and tocopherols from Tunisian pomegranate (Punica granatum) fruits. J Food Sci 2011, 76: 707 – 713.

19. Matthaiou MC, Goutzourelas N, Stagos D, Sarafoglou E, Jamurtas A, Koulocheri SD, Tsatsakis AM, Kouretas D: Pomegranate juice consumption increases GSH levels and reduces lipid and protein oxidation in human blood. Food Chem Toxicol 2014, 73:1-6.

20. Barros L, Carvalho AM, Sà Morais J, Ferreira ICFR: Strawberry-tree, blackthorn and rose fruits. Detailed characterisation in nutrients and phytochemicals with antioxidant properties. Food Chem 2010, 120:247-254.

21. Slater TF. Overview of methods used for detecting lipid peroxidation. Methods Enzymol 1984, 105:283–293.

22. Thirumalai T, Therasa SV, Elumalai EK, David E: Intense and exhaustive exercise induce oxidative stress in skeletal muscle. Asian Pacific Journal of Tropical Disease 2011, 63 – 66.

23. Azizbeigi K, Stannard SR., Atashak S, Haghighi MM: Antioxidant enzymes and oxidative stress adaptation to exercise training: comparison of endurance, resistance, and concurrent training in untrained males. Journal of Exercise Science & Fitness 2014, 12:1-6.

24. Chiaradia E, Avellini L, Rueca F, Spaterna A, Porciello F, Antonioni MT, Gaiti A: Physical exercise, oxidative stress and muscle damage in racehorses. Comp Biochem Physiol B 1998, 119:883-836.

25. Jović S, Stevanović J, Borozan S, Dimitrijević B, Milosavljević P: Influence of physical activity of racehorses on lactate dehydrogenase and creatine kinase activities, and protein synthesis. Acta Vet (Belgrade) 2013, 63:549-568.

26. Jović S, Stevanović J, Borozan S, Dimitrijević B, Popović T, Blagojević M, Lipid status in racehorses following physical activity of various intensity and duration. Acta Vet (Belgrade) 2013, 63:211-226.

27. Katalinic V, Milos M, Kulisic T, Jukic M: Screening of 70 medicinal plant extracts for antioxidant capacity and total phenols. Food Chem 2006, 94:550–557.

28. Morillas-Ruiz JM, Villegas Garcia JA, López FJ, Vidal-Guevara ML, Zafrilla P: Effects of polyphenolic antioxidants on exercise-induced oxidative stress. Clin Nutr 2006, 25:444-453.

29. Jagrič MS, Nemec SA, Zrimšek P, Kramarič P, Kos KV, Vovk T, Kobal S: Plasma malondialdehyde, biochemical and haematological parameters in standardbred horses during a selected field exercise test. Acta Vet (Belgrade) 2012, 62:53-65.

30. Gomez-Cabrera MC, Domenech E, Vina J: Moderate exercise is an antioxidant. Upregulation of antioxidant genes by training. Free Radical Biol Med 2008, 44:126-131.

31. Macedo RCS, Vieira A, Marin DP, Otton R: Effects of chronic resveratrol supplementation in military firefighters undergo a physical fitness test – A placebo controlled, double blind study. Chem Biol Interact 2015, 227:89-95.

32. Leenen R, Roodenburg AJC, Tijburg LBM, Wiseman SA: A single dose of tea with or without milk increases plasma antioxidant activity in humans. Eur J Clin Nutr 2000, 54:87-92.

33. Watson TA, Callister R, Taylor RD, Sibbritt DW, McDonald-Wicks LK, Garg ML:. Antioxidat restriction and oxidative stress in short-duration exhaustive exercise. Med Sci Sports Exerc 2005, 37:63-71.

34. McAnulty SR, McAnulty LS, Nieman DC, Morrow JD, Utter AC, Dumke CL: Effect on resistance exercise and carbohydrate ingestion on oxidative stress. Free Radic Res 2005, 39:1219 –1224.

35. Panza VSP, Wazlawik E, Schutz GR, Comin L, Hecht KC, da Silva EL:. Consumption of green tea favorably affects oxidative stress markers in weight-trained men. Nutrition 2008, 24:433-442.

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