This pilot-study evaluated the toxicity and safety profile of two herbal products Souroubea spp Botanical Blend (SSBB) and Platanus Tree Bark (PTB) after oral administration to dogs at elevated doses for 28 days. SSBB and PTB botanicals are the major active ingredients of Sin Susto™, a novel natural product for the treatment of anxiety in dogs. Three healthy female dogs were administered elevated doses of either SSBB, PTB or a placebo and then monitored for the occurrence of any systemic and local adverse events. Data from this pilot-study revealed that SSBB and PTB had no untoward effects on the health of dogs and were deemed safe which enabled the design and execution of a larger controlled target safety and toxicology study for Sin Susto™.
1. Sherman BL, Mills DS: Canine anxieties and phobias: an update on separation anxiety and noise aversions. The Veterinary clinics of North America Small animal practice 2008, 38(5):1081-1106, vii.
2. S hull-Selcer EA, Stagg W: Advances in the understanding and treatment of noise phobias. The Veterinary clinics of North America Small animal practice 1991, 21(2):353-367.
3. D reschel NA: The effects of fear and anxiety on health and lifespan in pet dogs. Applied Animal Behaviour Science 2010, 125:157-162.
4. J oseph A. Araujoa b, Christina de Riveraa,b, Jennifer L. Ethierb, Gary M. Landsbergb,c,, Sagi Denenbergc SA, Norton W. Milgrama, : Anxitane tablets reduce fear of human beings in a model of anxiety-related behavior. Journal of Veterinary Behavior 2010, 5:268-275.
5. M ullally M, Kramp K, Cayer C, Saleem A, Ahmed F, McRae C, Baker J, Goulah A, Otorola M, Sanchez P et al: Anxiolytic activity of a supercritical carbon dioxide extract of Souroubea sympetala (Marcgraviaceae). Phytotherapy research : PTR 2011, 25(2):264-270.
6. C ayer C: In vivo behavioural characterization of anxiolytic botanicals: Souroubea sympetala. University of Ottawa, Ottawa: University of Ottawa, Ottawa; 2011.
7. P isha E, Chai H, Lee IS, Chagwedera TE, Farnsworth NR, Cordell GA, Beecher CW, Fong HH, Kinghorn AD, Brown DM et al: Discovery of betulinic acid as a selective inhibitor of human melanoma that functions by induction of apoptosis. Nature medicine 1995, 1(10):1046-1051.
8. S imone Fulda M, Klaus-Michael Debatin: Betulinic acid induces apoptosis through a direct effect on mitochondria in meuroectodermal tumors. Medical and Pediatric Oncology 2000, 35:616-618.
9. F ujioka T, Kashiwada Y, Kilkuskie RE, Cosentino LM, Ballas LM, Jiang JB, Janzen WP, Chen IS, Lee KH: Anti-AIDS agents, 11. Betulinic acid and platanic acid as anti-HIV principles from Syzigium claviflorum, and the anti-HIV activity of structurally related triterpenoids. Journal of natural products 1994, 57(2):243-247.
10. Kashiwada Y, Hashimoto F, Cosentino LM, Chen CH, Garrett PE, Lee KH: Betulinic acid and dihydrobetulinic acid derivatives as potent anti-HIV agents. Journal of medicinal chemistry 1996, 39(5):1016-1017.
11. Mukherjee PK, Saha K, Das J, Pal M, Saha BP: Studies on the anti-inflammatory activity of rhizomes of Nelumbo nucifera. Planta medica 1997, 63(4):367-369.
12. Chandramu C, Manohar RD, Krupadanam DG, Dashavantha RV: Isolation, characterization and biological activity of betulinic acid and ursolic acid from Vitex negundo L. Phytotherapy research : PTR 2003, 17(2):129-134.
13. DP. L: Development and Validation of a Body Condition Score System for Dogs. Canine Practice 1997, 22:10-15.
14. Smith RD, Ristic M, Huxsoll DL, Baylor RA: Platelet kinetics in canine ehrlichiosis: evidence for increased platelet destruction as the cause of thrombocytopenia. Infection and immunity 1975, 11(6):1216-1221.
15. Romero LE, Meneses AI, Salazar L, Jimenez M, Romero JJ, Aguiar DM, Labruna MB, Dolz G: First isolation and molecular characterization of Ehrlichia canis in Costa Rica, Central America. Research in veterinary science 2011, 91(1):95-97.