Antimicrobial prophylaxis in addition to premedication in gastrointestinal surgery

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The aim of this study was to evaluate three combinations of antibiotics applied perorally prior to laparotomy and gastrointestinal surgery and to select the most favorable combination. Research was performed on 24 female and male dogs divided into four groups. Following abdominal wall incision the caecum was compressed manually and 5 mL of saline solution was injected into it. Five mL of caecum contents was aspirated in a sterile syringe and only 0.1 mL was incubated into blood agar and homogenous agar. Bacterial colonies were counted and determined. Prior to surgery the control group (n=6) received no antibiotics. The group which received the combination of gentamicin and clindamycin (n=6) achieved the best reduction of E. coli whereas Enterococcus faecals was 100% destroyed. The group which received amoxicillin with clavulanic acid and metronidazole (n=6) acquired a reduction of bacteria Enterococcus faecalis, which could be sufficient for prophylaxis. The combination of gentamicin and erythromycin (n=6) failed to accomplish a reduction of the number of bacteria.


  • 1. Suchodolski JS: Companian animals symposium: Microbes and gastrointestinal health of dogs and cats. J Anim Sci 2011, 89:1520-1530.

  • 2. Allen DA, Smeak DD, Schertel ER: Prevalence of small intestinal dehiscence and associated clinical factors: a retrospective study of 121 dogs. J Am Anim Hosp Ass 1992, 28:70-76.

  • 3. Culp WNT, Zeldis TE, Reese MS, Drobatz KJ: 2009, Primary bacterial peritonitis in dogs and cats: 24 cases (1990-2006). JAVMA 2009, 234:906-912.

  • 4. Swayne SL, Brisson B, Weese JS, Sears W: Evaluating the effect of intraoperative peritoneal lavage on bacterial culture in dogs with suspected septic peritonitis. Can Vet J 2012, 53:971-977.

  • 5. Lanz OI, Ellison GW, Bellah JR, Welcham J, VanGilder J: Surgical treatment of septic peritonitis without abdominal drainage in 28 dogs. J Am Anim Hosp Assoc 2001, 37:87-92.

  • 6. Bradford K, Meinkoth J, McKeimen K, Love B: Candida peritonitis in dogs: report of 5 cases. Vet Clin Pathol 2013, 42:227-233.

  • 7. Hedlund GL: Neuroradiology of the central nervous system in childhood. Neurol Clin, 2002, 20:965-981.

  • 8. Dunning D: Surgical Wound Infection and the use of Antimicrobials. In: Textbook of Small Animal Surgery, Saunders; 2003, 113-122.

  • 9. Gordon SM: Antibiotic prophylaxis against postoperative wound infections. Cleve Clin J Med 2006, 73:42-45.

  • 10. Chou S, Richards GK, Brown RA: A new approach to antibiotic therapy in colon surgery based on bioassay tissue concentrations. Can J Surg 1982, 25:527-531.

  • 11. Mansell JW: Biopsy of the gastrointestinal tract. Vet Clin Small Anim 2003, 33:1099-1116.

  • 12. Keats MM, Weeren R, Greenlee P, Evans KL, Minihan AC: Investigation of Keyes skin biopsy instrument for intestinal biopsy versus a standard biopsy technique. J Am Anim Hosp Ass 2004, 40:405-410.

  • 13. Cioffi KM, Schmiedt CW, Cornell KK, Radlinsky MG: 2012, Retrospective evaluation of vacuum-assisted peritoneal drainage for the treatment of septic peritonitis in dogs and cats: 8 cases (2003-2010). J Vet Emerg Crit Care 2012, 22:601-609.

  • 14. Tobias, KM: Surgery of the Digestive System. In: Manual of Small Animal Soft Tissue Surgery, Willey-Blackwell; 2010, 123-198.

  • 15. Weisman DL, Smeak DD, Birchard, SJ, Zweigart, SL: Comparison of a continuous suture pattern with a simple interrupted pattern for enteric closure in dogs and cats. J Am Vet Med Ass 1999, 214:1507-1510.

  • 16. Fossum TW: Surgery of the Abdominal Cavity. In: Small Animal Surgery, Mosby, Inc. St. Louis, IV edition; 2013, 356-386.

  • 17. Washabau RJ, Holt DE: Patophysiology of Gastrointestinal Disease. In: Textbook of Small Animal Surgery, Saunders; 2003, 530-552.

  • 18. Hawrelak JA, Myers SP: The Causes of Intestinal Dysbiosis: A Review. Altern Med Rev 2004, 9: 180-197.

  • 19. Holle GE, Steinbach E, Forth W: Effects of erythromycin in the dog upper gastrointestinal tract. Am J Physiol Gastrointest Liver Physiol 1992, 263:52-59.

Acta Veterinaria

The Journal of University of Belgrade

Journal Information

CiteScore 2016: 0.65

SCImago Journal Rank (SJR) 2016: 0.388
Source Normalized Impact per Paper (SNIP) 2016: 0.605


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