Coagulase-positive staphylococci are the most common pathogen causing subclinical mastitis in cows. Their main characteristic is a high virulence which leads to chronic infection. A total of 213 isolates of coagulase-positive staphylococci were tested. The majority of isolates (58%) formed a gold pigment, then light gold (28%), white gold (8%), golden gray, creamy white and white (2%). The majority of isolated coagulase-positive staphylococci produce beta hemolysis on esculin blood agar (50%), alpha and beta hemolysis (36%), beta and delta hemolysis (8%), delta hemolysis (4%), and alpha hemolysis (2%). Biochemical and molecular identification was performed by APISTAPH and multiplex PCR method. The majority of isolates were identified as S. aureus (88%), S. chromogenes (4%), and 2% of the isolates were identified as S. lentus, S. sciuri, S. xylosus, S. intermedius by APISTAPH. Antimicrobial susceptibility to penicillin G, was found by the Kirby Bauer disk diffusion method to be resistant (62.44%). For disc diffusion penicillin G sensitive isolates the minimal inhibitory concentration (MIC) was established for MIC50 and MIC90 as 0.003 mg/ml and 2μg/ml, respectively. For disc diffusion penicillin G resistant isolates MIC50 and MIC90 was 1.0μg/ml and 16μg/ ml, respectively. The study of phenotypic resistance to methicillin, as recommended by CLSI, established resistance to oxacillin in 5.26% of the isolates, while no resistance was found to cefoxitine. None of the tested isolates have the mecA gene.
1. Malinowski E, Lassa H, Kłlossowska A, Smulski S, Markiewicz H, Kaczmarowski M: Etiological agents of dairy cows’ mastitis in western part of Poland. Pol J Vet Sci 2006, 9(3):191-4.
2. Gianneechini RE, Concha C and Franklin A: Antimicrobial susceptibility of udder pathogens isolated from dairy herds in the west littoral region of Uruguay. Acta Vet Scand 2002, 43:31-41.
3. Wileland B, Feil C, Gloria-Maercker E, Thumm G, Lechner M, Bravo JM, Poralla K, Gotz F: Genetic and biochemical analyses of the biosynthesis of the yellow carotenoid 4,4’-diaponeurosporene of Staphylococcus aureus. J Bacteriol 1994, 176(24):7719-7726.
4. Begum HA, Uddin MS, Islam MJ, Nazir KHMNH, Islam MA, Rahman MT: Detection of biofi lm producing coagulase positive Staphylococcus aureus from bovine mastitis, their pigment production, hemolytic activity and antibiotic sensitivity pattern. J Bangladesh Soc Agric Sc. Technol 2007, 4(1-2): 97-100.
5. Ebrahimi A, Akhavan TM: Characteristics of staphylococci isolated from clinical and subclinical mastitis cows in Shahrekord, Iran. Iran J Vet Res 2009, 10(28): 273-277.
6. Franco G, Julio C, González V Libertad, Gómez M, Sandra C, Carrillo G, Juan M, Ramírez C, José J: Virulence factors analysis of Staphylococcus aureus isolated from bovine mastitis in México. e-Gnosis 2008, 6:1-9.
7. Roberson JR, Fox LK, Hancock DD, Gay JM, Besser TE: Prevalence of coagulase-positive staphylococci, other than Staphylococcus aureus, in bovine mastitis. Am J Vet Res 1996, 57(1):54-8.
8. Capurro A, Concha C, Nilsson L, Ostensson K: Identifi cation of coagulase-positive staphylococci isolated from bovine milk. Acta Vet Scand 1999, 40:315-21.
9. National mastitis council research committee report bovine mastitis pathogens and trends in resistance to antibacterial drugs. NMC Annual Meeting Proceedings. 2004, 400-414.
10. Malinowski E, Klossowska A, Kaczmarowski M, Lassa H, Kuzma K: Antimicrobial susceptibility of staphylococci isolated from affected with mastitis cow. Bull Vet Inst Pulawy 2002, 46:289-94.
11. Hendriksen RS, Mevius DJ, Schroeter A, Teale C, Meunier D, Butaye P, Franco A, Utinane A, Amado A, Moreno M, Greko C, Strk K, Berghold C, Myllyniemi AL, Wasyl D, Sunde M, Aarestrup FM: Prevalence of antimicrobial resistance among bacterial pathogens isolated from cattle in different European countries: 2002-2004. Acta Vet Scand 2008, 8(50):28.
12. Moon JS, Lee AR, Jaw SH, Kang HM, Joo YS, Park YH, Kim MN, Koo HC: Comparison of antibiogram, staphylococcal enterotoxin productivity, and coagulase genotypes among Staphylococcus aureus isolated from animal and vegetable sources in Korea. J Food Prot 2007, 70:2541-2548.
13. Turutoglu H, Ercelik S, Ozturk D: Antibiotic resistance of Staphylococcus aureus and coagulasenegative staphilococci isolated from bovine mastitis. Bull Vet Inst Pulawy 2006, 50:41-45.
14. Europen Union Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from humans, animals and food in 2010. European Food Safety Authority 2, 3 European Centre for Disease Prevention and Control, EFSA Journal 2012, 10(3): 2598.
15. CLSI (Clinical and Laboratory Standard Institute): Performance standards for antimicrobial susceptibility testing; sixteenth informational supplement. 2006, January 26(3).
16. Takeshige K, Watanabe K, Igarashi H, Shingaki M, Terayama T: Detection of Staphylococcus aureus in bovine mastitis and some characteristics with special reference to enterotoxin producibility and coagulase types of isolates. Nihon JuigakuZasshi 1983, 45(3):355-362.
17. Silva Padilha W, Destro MT: Biochemical characteristic of typical and atypical Staphylococcus aureus in mastitic milk and environmental samples of Brazilian dairy farms. Braz. J. Microbiol 2000, 31(2):103-106.
18. Da Costa GM, Vilela Paiva L, Hilsdorf Piccoli R, Figueiredo Demétrio J, Ulisses de Pádua P, da SilvaN: Evaluation of a simplifi ed key for the identifi cation of coagulase-positive Staphylococcus isolated from bovine mastitis. Acta Biol Sci 2010, 32(4):403-406.
19. Langlos EB, Robert J. Harmon and Akers K: Identifi cation of Staphylococcus Species of Bovine Origin with the API Staph-Ident System. J Clin Microbiol 1983, 18:1212-1219.
20. Güler L, Ok Ü, Gündüz K, Gülcü Y, Hadimli HH: Antimicrobial susceptibility and coagulase gene typing of Staphylococcus aureus isolated from bovine clinical mastitis cases in Turkey. J Dairy Sci 2005, 88(9):3149-3154.
21. Kalmus P, Birgit A, Age K, Toomas O, Kalle K: Udder pathogens and their resistance to antimicrobial agents in dairy cows in Estonia. Acta Vet Scand 2011, 53:4.
22. Turutoglu H, Mudul S, Pehlivanoglu F: Antibiotic susceptibility and β-actamase prevalence for staphylococci isolated from bovine mastitic milk samples. Acta Vet (Beograd) 2002, 52:337-344.
23. Bengtsson B , Helle EU, Torkel E, Karin A, Nilsson-ÖstM, Karin PW: Antimicrobial susceptibility of udder pathogens from cases of acute clinical mastitis in dairy cows. Vet Microbiol 2009, 136:142-149.
24. Kirkan S, Goksoy EO, Kaya O: Identifi cation and antimicrobial susceptibility of Staphylococcus aureus and coagulase negative staphylococci from bovine mastitis in the aydýn region of Turkey. J Vet Anim Sci 2005, 29:791-96.
25. Watts J, Yancey RJ: Comparison of success of antibiotic therapy during lactation and results of antimicrobial susceptibility tests for bovine mastitis. J Dairy Sci 1997, 80(2):313-317.
26. Kaya EG, Esra K, Serap Y, Mihriban Y: Evaluation of phenotypic and genotypic methods for detection of methicillin resistance in Staphylococcus. African J Microbiol Res 2009, 3(12): 925-29.
27. Saini V, McClure JT, Scholl DT, DeVries TJ, Barkema HW: Herd-level association between antimicrobial use and antimicrobial resistance in bovine mastitis staphylococcus aureus isolates on Canadian dairy farms. J Dairy Sci 2012, 95:1921-1929
28. Moon JS, Lee AR, Kang HM, Lee ES, Kim MN, Paik YH, Park YH, Joo YS, Koo HC: Phenotypic and Genetic Antibiogram of Methicillin-Resistant Staphylococci Isolated from Bovine Mastitis in Korea. J. Dairy Sci 2009, 90:1176-85.