Peels, seeds and leaves of the Sea buckthorn were extracted by methanol and chloroform and characterized in terms of the fatty acids (FA) and tocopherol composition. All morphological parts of Sea buckthorn contained these biologically active compounds useful in medicine, pharmacology, human nutrition and cosmetics. The highest amount of α-tocopherol was found in peels (1103 mg kg-1). The lowest content of α-tocopherol was found in leaves (659 mg kg-1). Delta-tocopherol was found in higher amount in peels 1757 mg kg-1. Seeds contained 95 mg kg-1 of delta-tocopherols. Gamma-tocopherol was found in seeds (459 mg kg-1), peels (188 mg kg-1) and in leaves (587 mg kg-1). β-tocopherol was present only in seeds (171 mg kg-1). Unsaturated fatty acids were dominant in all morphological parts of Sea buckthorn. The highest amounts of unsaturated fatty acids (92 rel. %) were determined in seeds. Dominant fatty acids of seeds were linoleic acid (37 %), α-linolenic acid (30 %) and vaccenic acid (20 %). Leaves were rich in α-linolenic acid (51 %). Dominant fatty acids of peels were oleic acid (16 %), palmitic acid (33 %) and palmitoleic acid (29 %).