Simultaneous spectrofluorometric analysis of tablets containing hydrochlorothiazide combined with timolol maleate or amiloride hydrochloride

Fadel Wedian 1 , Anas Lataifeh 2  and Makaseb S. Mohammed 1
  • 1 Department of Chemistry Faculty of Science, Irbid, Jordan
  • 2 Department of Chemistry Faculty of Science, 66110, Jordan

Abstract

Green and sensitive spectrofluorometric methods have been developed and validated for the determination of timolol maleate (TML)/hydrochlorothiazide (HCT) and amiloride hydrochloride (AMH)/hydrochlorothiazide in tablets. The proposed spectrofluorometric procedures were found to be linear in the range of 4–12, 5–35 and 0.025–0.2 mg L−1 for HCT, TML and AMH, resp. The excitation and emission wavelengths for HCT, TML and AMH at room temperature were 270 and 375, 295 and 435, 330 and 415 nm, resp. The methods were validated with respect to ICH guidelines. The AMH showed higher sensitivity with lower values of LOD and LOQ values compared to HCT and TML. The proposed methods were applied to two pharmaceutical formulations; the method for HCT and AMH has proven as reliable assaying method, whereas the method for TML, when combined with HCT, is applicable to screening semi-quantitative analyses.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. M. C. F. Ferraro, P. M. Castellano and T. S. Kaufman, Simultaneous determination of amiloride hydrochloride and hydrochlorothiazide in synthetic samples and pharmaceutical formulations by multivariate analysis of spectrophotometric data, J. Pharm. Biomed. Anal. 30 (2002) 1121–1131; https://doi.org/10.1016/s0731-7085(02)00420-x

  • 2. B. G. Katzung, S. B Masters and A. J. Trevor, Basic and Clinical Pharmacology, 13th ed, McGraw-Hill, New York 2012.

  • 3. D. J. Mazzo, Analytical Profile of Drug Substances, Academic Press, London 1986, Vol. 15, pp. 134.

  • 4. M. Kartal and N. Erk, Simultaneous determination of hydrochlorothiazide and amiloride hydro-chloride by ratio spectra derivative spectrophotometry and high-performance liquid chromatography, J. Pharm. Biomed. Anal. 19 (1999) 477–485; https://doi.org/10.1016/s0731-7085(98)00241-6

  • 5. J. Rouland, P. Morel-Mandrino, P. Elena, H. Polzer and P. Sunder Raj, Timolol 0.1% gel (Nyogel 0.1%®) once daily versus conventional timolol 0.5% solution twice daily: A comparison of efficacy and safety, Ophthalmologica 216 (2002) 449–454; https://doi.org/10.1159/000067548

  • 6. N. Erk and F. Onur, Three new spectrophotometric methods for simultaneous determination of hydrochlorothiazide and amiloride hydrochloride in sugar-coated tablets, Anal. Lett. 30 (1997) 1503–1515; https://doi.org/10.1080/00032719708001671

  • 7. C. V. N. Prasad, C. Parihar, K. Sunil and P. Parimoo, Simultaneous determination of amiloride HCl, hydrochlorothiazide and atenolol in combined formulations by derivative spectroscopy, J. Pharm. Biomed. Anal. 17 (1998) 877–884; https://doi.org/10.1016/s0731-7085(97)00241-0

  • 8. M. H. Abdel-Hay, A. A. Gazy, E. M. Hassan and T. S. Belal, Derivative and derivative ratio spec-trophotometric analysis of antihypertensive ternary mixture of amiloride hydrochloride, hydro-chlorothiazide and timolol maleate, J. Chin. Chem. Soc. 55 (2008) 971–978; https://doi.org/10.1002/jccs.200800144

  • 9. M. E. Martín, O. M. Hernández, A. I. Jiménez, J. J. Arias and F. Jiménez, Partial least-squares method in analysis by differential pulse polarography. Simultaneous determination of amiloride and hydrochlorothiazide in pharmaceutical preparations, Anal. Chim. Acta 381 (1999) 247–256; https://doi.org/10.1016/s0003-2670(98)00732-6

  • 10. M. Zecevic, L. J. Zivanovic, S. Agatonovic-Kustrin, D. Ivanovic and M. Maksimovic, Statistical optimization of a reversed-phase liquid chromatographic method for the analysis of amiloride and hydrochlorothiazide in tablets, J. Pharm. Biomed. Anal. 22 (2000) 1–6; https://doi.org/10.1016/s0731-7085(99)00253-8

  • 11. M. C. F. Ferraro, P. M. Castellano and T. S. Kaufman, Chemometric determination of amiloride hydrochloride, atenolol, hydrochlorothiazide and timolol maleate in synthetic mixtures and pharmaceutical formulations, J. Pharm. Biomed. Anal. 34 (2004) 304–314; https://doi.org/10.1016/s0731-7085(03)00521-1

  • 12. A. A. Elshanawane, L. M. Abdelaziz, M. S. Mohram and H. M. Hafez, Development and validation of HPLC method for simultaneous estimation of brimonidine tartrate and timolol maleate in bulk and pharmaceutical dosage form, J. Chromatogr. Sep. Tech. 5 (2014) Article ID 1000230 (5 pages); https://doi.org/10.4172/2157-7064.1000230

  • 13. F. P. Bigley, R. L. Grob and G. S. Brenner, Pharmaceutical applications of a high-performance flow injection system, Anal. Chim. Acta 181 (1986) 241–244; https://doi.org/10.1016/s0003-2670(00)85240-x

  • 14. M. Kaljurand and M. Koel, Recent advancements on greening analytical separation, Crit. Rev. Anal. Chem. 41 (2011) 2–20; https://doi.org/10.1080/10408347.2011.539420

  • 15. International Conference in Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use, ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2(R1), Current Step 4 version, ICH, November 2005; http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1_Guideline.pdf; last access: June 2018

  • 16. S. J. Kok, R. Evertsen, N. H. Velthorst, U. A. Th. Brinkman and C. Gooijer, On the coupling of fluorescence line-narrowing spectroscopy and poly(ethylene thin-layer chromatography)imine-cellulose, Anal. Chim. Acta 405 (2001) 1–7; https://doi.org/10.1016/S0003-2670(99)00698-4

  • 17. A. K. Aimukhanov and N. Kh. Ibrayev, Influence of overlapping between the fluorescence spectra of dye molecules and the spectrum of plasmon absorption of silver nanoparticles on the luminescence of laser dyes in ethyl alcohol, Eurasian J. Phys. Func. Mat. 2 (2018) 43–53; https://doi.org/10.29317/ejpfm.2018020105

  • 18. V. S. Bhadresh, H. A. Raj, S. Rajanit and S. Harshita, Analytical techniques for determination of hydrochlorothiazide and its combinations: A review, Int. J. Adv. Sci. Res. 1 (2015) 114–128; https://doi.org/10.7439/ijasr

  • 19. J. S. Shaikh and N. N. Rao, Simultaneous estimation and forced degradation studies of amiloride hydrochloride and furosemide in a pharmaceutical dosage form using reverse-phase high-performance liquid chromatography method, Asian J. Pharm. Clin. Res. 11 (2018) 215–221; https://doi.org/10.22159/ajpcr.2018.v11i7.25783

  • 20. P. Ortega-Barrales, G. Pellerano, F. A. Vazquez and A. Molina-Díaz, Rapid and sensitive determination of amiloride by cation exchange preconcentration and direct solid-phase UV detection, Anal. Lett. 35 (2002) 1491–1504; https://doi.org/10.1081/AL-120006725

  • 21. M. Walash and R. El-Shaheny, Fast separation and quantification of three anti-glaucoma drugs by high-performance liquid chromatography UV detection, J. Food Drug Anal. 24 (2016) 441–449; https://doi.org/10.1016/j.jfda.2015.11.006

  • 22. N. Erk, Simultaneous determination of dorzolamide HCl and timolol maleate in eye drops by two different spectroscopic methods, J. Pharm. Biomed. Anal. 15 (2002) 391–397; https://doi.org/10.1016/S0731-7085(01)00627-6

  • 23. S. Mahgoub, Novel stability-indicating RP-HPLC method for determination of hydrochlorothiazide, amiloride hydrochloride and timolol maleate in tablet dosage form, Am. J. Mod. Chromatogr. 3 (2016) 23–32; https://doi.org/10.7726/ajmc.2016.1002

OPEN ACCESS

Journal + Issues

Search