Testing of resveratrol microemulsion photostability and protective effect against UV induced oxidative stress

Open access


Resveratrol is well known for its antioxidant activity and susceptibility to ultraviolet radiation. Development of formulations providing improved stability and relevant drug delivery of resveratrol is still a challenging task. The aim of this study was to determine protective characteristics of formulated microemulsions by evaluating photoisomerization of resveratrol and to investigate the effects of resveratrol on human keratinocyte cells under oxidative stress caused by ultraviolet radiation. Incorporation of resveratrol into microemulsions resulted in increased photostability of active compounds and the results demonstrated that photodegradation of resveratrol was significantly delayed. Results of biopharmaceutical evaluation in vitro demonstrated that up to 60 % of resveratrol was released from microemulsions within 6 hours under a constant release rate profile. In vivo biological testing confirmed the ability of resveratrol to protect cells from oxidative stress and to increase cell viability. It was concluded that microemulsions might be considered in the development of UV light sensitive compounds.

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  • 1. M. Ndiaye C. Philippe H. Mukhtar and N. Ahmad The grape antioxidant resveratrol for skin disorders: Promise prospects and challenges Arch. Biochem. Biophys. 508 (2011) 164-170; DOI: 10.1016/j.abb.2010.12.030.

  • 2. R. A. Baxter Anti-aging properties of resveratrol: Review and report of a potent new antioxidant skin care formulation J. Cosmetic Dermatol. 2008 2-7; DOI: 10.1111/j.1473-2165.2008.00354.x.

  • 3. S. S. Leonard C. Xia B. H. Jiang B. Stinefelt H. Klandorf G. K. Harris and X. Shi Resveratrol scavenges reactive oxygen species and effects radical-induced cellular responses Biochem. Biophys. Res. Commun. 309 (2003) 1017-1026; DOI: 10.1016/j.bbrc.2003.08.105.

  • 4. C. B. Detoni G. D. Souto A. L. M. Da Silva A. R. Pohlmann and S. S. Guterres Photostability and skin penetration of different E-resveratrol-loaded supramolecular structures Photoc. Photobiol. 2012 913-921; DOI: 10.1111/j.1751-1097.2012.01147.x.

  • 5. K. Teskač and J. Kristl The evidence for solid lipid nanoparticles mediated cell uptake of resveratrol Int. J. Pharm. 390 (2009) 61-69; DOI: 10.1016/j.ijpharm.2009.10.011.

  • 6. C. Caddeo M. Manconi A. M. Fadda F. Lai S. Lampis O. Diez-Sales and C. Sinico Nanocarriers for antioxidant resveratrol: Formulation approach vesicle self-assembly and stability evaluation Coll. Surf. B. Biointerfaces 111 (2013) 327-332; DOI: 10.1016/j.colsurfb.2013.06.016.

  • 7. H. Y. Karasulu Microemulsions as novel drug carriers: the formation stability applications and toxicity Expert Opin. Drug Deliv. 5 (2008) 119-135; DOI: 10.1517/17425247.5.1.119.

  • 8. T. Wan T. Xu J. Pan M. Qin W. Pan G. Zhang Z. Wu C. Wu and Y. Xu Microemulsion based gel for topical dermal delivery of pseudolaric acid B: In vitro and in vivo evaluation Int. J. Pharm. 493 (2015) 111-120; DOI: 10.1016/j.ijpharm.2015.07.058.

  • 9. V. Juškaitė K. Ramanauskienė and V. Briedis Design and formulation of optimized microemulsions for dermal delivery of resveratrol eCAM 2015 1-10; DOI: 10.1155/2015/540916.

  • 10. S. Sapino M. E. Carlotti G. Caron E. Ugazio and R. Cavalli In silico design photostability and biological properties of the complex resveratrol/hydroxypropyl-β-cyclodextrin J. Incl. Phenom. Macrocycl. Chem. 63 (2009) 171-180; DOI: 10.1007/s10847-008-9504-7.

  • 11. K. Ramanauskiene A. Stelmakiene and D. Majiene Assessment of Lemon Balm (Melissa officinalis L.) Hydrogels: Quality and Bioactivity in Skin Cells eCAM 2015 1-7; DOI: 10.1155/2015/635975.

  • 12. N. Vitale A. Kisslinger S. Paladino C. Procaccini G. Matarese G. M. Pierantoni F. P. Mancini and D. Tramontano Resveratrol couples apoptosis with autophagy in UVB-irradiated HaCaT cells PLoS One. 8 (2013) e80728; DOI: 10.1371/journal.pone.0080728.

  • 13. A. Francioso A. Boffi C. Villani L. Manzi M. D’Erme A. Macone and L. Mosca Isolation and identification of 246-trihydroxyphenanthrene as a byproduct of trans-resveratrol photochemical isomerization and electrocyclization J. Org. Chem. 79 (2014) 9381-9384; DOI: 10.1021/jo501405m.

  • 14. P. Sasivimolphan V. Lipipun G. Ritthidej K. Chitphet Y. Yoshida T. Daikoku B. Sritularak K. Likhitwitayawuid P. Pramyothin M. Hattori and K. Shiraki Microemulsion-based oxyresveratrol for topical treatment of herpes simplex virus (HSV) infection: Physicochemical properties and efficacy in cutaneous HSV-1 infection in mice AAPS PharmSciTech. 13 (2012) 1266-1275; DOI: 10.1208/s12249-012-9828-x.

  • 15. J. Hao Y. Gao J. Zhao J. Zhang Q. Li Z. Zhao and Y. Liu Preparation and optimization of resveratrol nanosuspensions by antisolvent precipitation using Box-Behnken design AAPS PharmSci-Tech. 16 (2015) 118-128; DOI: 10.1208/s12249-014-0211-y.

  • 16. H. Chen and Q. Zhong Thermal and UV stability of β-carotene dissolved in peppermint oil microemulsified by sunflower lecithin and Tween 20 blend Food Chem. 174 (2015) 630-636; DOI: 10.1016/j.foodchem.2014.11.116.

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