TLC determination of flavonoids from different cultivars of Allium cepa and Allium ascalonicum

Loretta Pobłocka-Olech 1 , Daniel Głód 2 , Maria E. Żebrowska 3 , Małgorzata Sznitowska 4 , and Miroslawa Krauze-Baranowska 2
  • 1 Department of Pharmacognosy with Medicinal Plants Garden, Medicine Medical University of Gdańsk 80-416 Gdańsk, Poland
  • 2 Department of Pharmacognosy with Medicinal Plants Garden, Medical University of Gdańsk 80-416 Gdańsk, Poland
  • 3 Department of Pharmaceutical Technology, Faculty of Pharmacy with Subfaculty of Laboratory Medicine Medical University of Gdańsk 80-416 Gdańsk, Poland
  • 4 Department of Pharmaceutical Technology Faculty of Pharmacy with Subfaculty of Laboratory Medicine Medical University of Gdańsk 80-416 Gdańsk, Poland


This study comprises the optimization and validation of a new TLC method for determination of flavonols in the bulbs of seven cultivars of onions and shallots. Separation was performed on RP-18 plates with the solvent mixture tetrahydrofuran/water/formic acid (40+60+6, V/V/V) as a mobile phase. The method was evaluated for precision, linearity, LOD, LOQ, accuracy and robustness. Chromatographic analysis of the extracts revealed the presence of three main flavonols, quercetin, quercetin-4′-O-glucoside and quercetin-3,4′-O-diglucoside in the majority of analyzed cultivars. The content of flavonols in the analyzed extracts of onion bulbs varied from 123 (‘Exihibition’) to 1079 mg kg-1 fresh mass (fm) (‘Hybing’) in edible parts, and from 1727 (‘Hyline’) to 28949 mg kg-1 fm (‘Red Baron’) in outer scales. The bulbs of two shallot cultivars contained 209 (‘Ambition’) and 523 mg kg-1 fm (‘Matador’) of flavonols in edible parts and 5426 and 8916 mg kg-1 fm in outer scales, respectively.

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  • 1. P. Bonaccorsi, C. Carsti, C. Gargiulli and U. Leuzzi, Flavonol glucosides in Allium species: A comparative study by means of HPLC-DAD-ESI-MS-MS, Food Chem. 107 (2008) 1668-1673; DOI: 10.1016/j.foodchem.2007.09.053.

  • 2. V. Lanzotti, The analysis of onion and garlic, J. Chromatogr. A 1112 (2006) 3-22; DOI: 10.1016/j. chroma.2005.12.016.

  • 3. T. H. Soininen, N. Jukarainen, R. Julkunen-Tiitto, R. Karjalainen and J. J. Vepsäläinen, The combined use of constrained total-line-shape 1H NMR and LC-MS/MS for quantitative of bioactive components in yellow onion, J. Food Comp. Anal. 25 (2012) 208-214; DOI: 10.1016/j.jfca.2011.09.001.

  • 4. M. Pikuła, M. E. Żebrowska, L. Pobłocka-Olech, M. Krauze-Baranowska, M. Sznitowska and P. Trzonkowski, Effect of enoxaparin and onion extract on human skin fibroblast cell line - Therapeutic implications for treatment of keloids, Pharm. Biol. 52 (2014) 262-267; DOI: 10.3109/13880209.2013.826246.

  • 5. P. Bonaccorsi, C. Caristi, C. Gargiulli and U. Leuzzi, Flavonol glucoside profile of southern Italian red onion (Allium cepa L.), J. Agr. Food Chem. 53 (2005) 2733-2740; DOI: 10.1021/jf048152r.

  • 6. N. Beesk, H. Perner, D. Schwarz, E. George, L. W. Kroh and S. Rohn, Distribution of quercetin-3,4’- O-diglucoside, quercetin-4’-O-monoglucoside, and quercetin in different parts of the onion bulb (Allium cepa L.) influenced by genotype, Food Chem. 122 (2010) 566-571; DOI: 10.1016/j.foodchem. 2010.03.011.

  • 7. K. Grzelak, J. Milala, B. Król, F. Adamicki and E. Badełek, Content of quercetin glycosides and fructooligosaccharides in onion stored in a cold room, Eur. Food Res. Technol. 228 (2009) 1001-1007; DOI: 10.1007/s00217-009-1018-z.

  • 8. I. M. Vågen and R. Slimestad, Amount of characteristic compounds in 15 cultivars of onion (Allium cepa L.) in controlled field trials, J. Sci. Food Agr. 88 (2008) 404-411; DOI: 10.1002/jsfa.3100.

  • 9. M. Marotti and R. Piccaglia, Characterization of flavonoids in different cultivars of onion (Allium cepa L.), J. Food Sci. 67 (2002) 1229-1232; DOI: 10.1111/j.1365-2621.2002.tb09482.x.

  • 10. C. Cimpoiu, Analysis of some natural antioxidants by thin-layer chromatography and high performance thin-layer chromatography, J. Liq. Chromatogr. Relat. Technol. 29 (2006) 1125-1142; DOI: 10.1080/10826070600574911.

  • 11. T. Vanitha, H. Sumathy, J. Sangeetha, B. Devaki and K. Vijayalakshmi, Phytochemical analysis of Allium ascalonicum, Biomedicine 29 (2009) 22-25.

  • 12. M. Krauze-Baranowska and W. Cisowski,. Flavonoids from Echinocystis lobata and E. wrightii, Pol. J. Chem. 70 (1996) 430-436.

  • 13. K. R. Markham, Techniques of Flavonoids Identification, Academic Press, London 1982.

  • 14. I. Vovk, B. Simonovska, S. Andrenšek, T. Yrjönen, P. Vuorela and H. Vuorela, Rotation planar extraction and medium-pressure solid-liquid extraction of onion (Allium cepa), J. Planar Chromatogr. 16 (2003) 66-70; DOI: 10.1556/JPC.16.2003.1.14.

  • 15. L. Gennaro, Ch. Leonardi, F. Esposito, M. Salucci, G. Maiani, G. Quaglia and V. Fogliano, Flavonoid and carbohydrate contents in Tropea red onions: Effects of homelike peeling and storage, J. Agr. Food Chem. 50 (2002) 1904-1910; DOI: 10.1021/jf011102r.


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