Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract

Open access

Antioxidant and free radical scavenging potential of Citrullus colocynthis (L.) Schrad. methanolic fruit extract

Citrullus colocynthis (L.) Schrad. (Cucurbitaceae) is a medicinal plant traditionally used as an abortifacient and to treat constipation, oedema, bacterial infections, cancer and diabetes. Preliminary phytochemical screening of the plant showed the presence of large amounts of phenolics and flavonoids. Subsequent quantification showed the presence of 0.74% (m/m) phenolics (calculated as gallic acid) and 0.13% (m/m) flavonoids calculated as catechin equivalents per 100 g of fresh mass. The presence of phenolic compounds prompted us to evaluate its antioxidant activity. In the present study, methanolic fruit extract of C. colocynthis was screened to evaluate its freeradical scavenging effect. The highest antioxidant and free radical scavenging ability of the fruit extract was observed at a concentration of 2500 μg mL-1.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • B. Halliwell and J. M. C. Gutteridge Free Radicals in Biology and Medicine 3rd ed. Oxford University Press Oxford 1999.

  • T. Finkel and N. J. Holbrook Oxidants oxidative stress and the biology of aging Nature 408 (2000) 239-247; DOI:10.1038/35041687.

  • T. P. A. Devasagayam J. C. Tilak K. K. Boloor K. S. Sane S. S. Ghaskadbi and R. D. Lele Review: Free radicals and antioxidants in human health: Current status and future prospects J. Assoc. Phys. India 52 (2004) 794-804.

  • A. L. Miller Antioxidant flavonoids: structure function and clinical usage Alt. Med. Rev. 1 (1996) 103-111.

  • A. Delazar S. Gibbons A. R. Kosari H. Nazemiyeh M. Modarresi L. Nahar and S. D. Sarker Flavone C-glycosides and cucurbitacin glycosides from Citrullus colocynthis DARU 14 (2006) 109-114.

  • V. L. Singleton and J. A. Rossi Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents Am. J. Enol. Vitic. 16 (1965) 144-158.

  • D. Marinova F. Ribarova and M. Atanasova Total phenolics and flavonoids in Bulgarian fruits and vegetables J. Univ. Chem. Tech. Metall. 40 (2005) 255-260.

  • N. Sreejayan and M. N. A. Rao Free radical scavenging activity of curcuminoids Drug Res. 46 (1996) 169-171.

  • S. M. Klein G. Cohen and A. I. Cederbaum Production of formaldehyde during metabolism of dimethylsulphoxide by hydroxyl radical generating system Biochemistry 20 (1981) 6006-6012.

  • I. Gülçin H. A. Alici and M. Cesur Determination of in vitro antioxidant and radical scavenging activities of propofol Chem. Pharm. Bull. 53 (2005) 281-285; DOI: 10.1248/cpb.53.281.

  • M. Nishikimi N. A. Rao and K. Yagi The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen Biochem. Biophys. Res. Commun. 46 (1972) 849-853.

  • R. Govindarajan S. Rastogi M. Vijayakumar A. Shirwaikar A. K. Rawat S. Mehrotra and P. Pushpangadan Studies on antioxidant activities of Desmodium gangeticum Biol. Pharm. Bull. 26 (2003) 1424-1427; DOI: 10.1248/bpd.26.1424.

  • L. W. Aurand N. H. Boonme and G. G. Gidding Superoxide and singlet oxygen in milk lipid peroxidation J. Dairy Sci. 60 (1977) 363-369.

  • A. E. Hagerman K. M. Riedl G. A. Jones K. N. Sovik N. T. Ritchard P. W. Hartzfeld and T. L. Riechel High molecular weight plant polyphenolics (tannins) as biological antioxidants J. Agric. Food Chem. 46 (1998) 1887-1892; DOI: 10.1021/jf970975b.

Search
Journal information
Impact Factor

IMPACT FACTOR 2018: 1.405
5-year IMPACT FACTOR: 1.701

CiteScore 2018: 1.47

SCImago Journal Rank (SJR) 2018: 0.314
Source Normalized Impact per Paper (SNIP) 2018: 0.637

Cited By
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 856 416 16
PDF Downloads 258 160 7