A sensitive spectrophotometric method for the determination of sulfonamides in pharmaceutical preparations

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A sensitive spectrophotometric method for the determination of sulfonamides in pharmaceutical preparations

A new, simple and sensitive spectrophotometric method for the determination of some sulfonamide drugs has been developed. The method is based on the diazotization of sulfacetamide, sulfadiazine, sulfaguanidine, sulfamerazine, sulfamethazine, sulfamethoxazole, and their coupling with 8-hydroxyquinoline in alkaline media to yield red coloured products with absorption maxima at 500 nm. Beer's law is obeyed from 0.1--7.0 μg mL-1. The limits of quantification and limits of detection were 0.11--0.18 and 0.03--0.05 μg mL-1, respectively. Intraday precision (RSD 0.1--0.5%) and accuracy (recovery 97.3--100.8%) of the developed method were evaluated. No interference was observed from common adjuvants. The method has been successfully applied to the assay of sulpha drug in pharmaceutical formulations.

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  • L. A. Mitscher Antibiotics and Antimicrobial Agents in Foye's Principles of Medicinal Chemistry (Eds. D. A. Williams and T. L. Lemke) 5th ed. Lippincott Williams and Wilkins Philadelphia 2002 pp. 819--862.

  • W. A. Petri Jr. Antimicrobial Agents in Goodman and Gilman's Pharmacological Basis of Therapeutics (Eds. J. G. Hardman and Lee E. Limbird) 10th Ed. McGraw Hill New York 2001 pp. 1171--1188.

  • J. M. Beale Jr. Anti Infective Agents in Wilson and Gisvold's Text Book of Organic Medicinal and Pharmaceutical Chemistry (Eds. J. H. Block and J. M. Beale Jr.) 11th ed. Lippincott William and Wilkins Philadélphia 2004 pp. 217--281.

  • British Pharmacopoeia Vol II Her Majesty's Stationery Office London 2004 pp. 1858--1859.

  • B. Chivarino M. A. Crestoni A. Di-Marzio and S. Fornarini Determination of sulfonamide antibiotics by gas chromatography coupled with atomic emission detection J. Chromatogr. Biomed. Appl.706 (1998) 269--277; DOI: 10.1016/S0378--4347(97)00568--9.

  • F. M. El Anwar A. M. El Walily M. H. Abdel Hay and M. El Swify The analysis of a triple sulfonamide in pharmaceutical powder form by HPLC Anal. Lett.24 (1991) 767--779; DOI: 10.1080/00032719108052941.

  • G. K. Thomas R. G. Millar and P. W. Antis Stability of sulfonamide antibiotics in spiked pig liver tissue during frozen storage JAOAC Int.80 (1997) 988--995.

  • G. Knupp H. Pollmann and D. Jonas An improved HPTLC method for the rapid identification and quantification of sulfonamides Chromatographia22 (1986) 21--24; DOI: 10.1007/BF02257291.

  • C. L. Flurer Analysis of antibiotics by capillary electrophoresis Electrophoresis18 (1997) 2427--2437; DOI: 10.1002/elps.1150181233.

  • F. Malecki and V. Starosak Potentiometric determination of sulfonamides with a silver sulfide electrode Anal. Chim. Acta139 (1982) 353--357; DOI:10.1016/S0003--2670(01)94015-2.

  • M. T. Ackermans J. L. Beckers F. M. Everaerts H. Hoogland and M. J. H. Tomassen Determination of sulphonamides in pork meat extracts by capillary zone electrophoresis J. Chromatogr.596 (1992) 101--109; DOI: 10.1016/0021-9673(92)80209-D.

  • T. A. M. Msagati and J. C. Ngila Voltammetric detection of sulfonamides at a poly(3-methylthiophene) electrode Talanta58 (2002) 605--610; DOI: 10.1016/S0039-9140(02)00327-2.

  • E. Martlbauer E. Usleber E. Schneider and R. Dietrich Immunochemical detection of antibiotics and sulfonamides Analyst119 (1994) 2543--2548; DOI: 10.1039/AN9941902543.

  • S. W. Garden and P. Sporns Development and evaluation of an enzyme immunoassay for sulfamerazine in milk J. Agric. Food Chem.42 (1994) 1379--1391; DOI: 10.1021/jf00042a026.

  • M. S. Pena F. Salinas M. C. Mahendero and J. J. Aaron Spectrofluorimetric determination of sulphonamides in pharmaceutical compounds and foods J. Pharm. Biomed. Anal.10 (1992) 805--808; DOI:10.1016/0731-7085(91)80084-M.

  • Y. M. Issa A. L. El. Ansary and W. Selim Enthalpimetric determination of sulfa drugs in pure form and pharmaceutical formulations Anal. Lett.31 (1998) 131--146; DOI: 10.1080/00032719808001838.

  • W. S. Sutherland J. J. Laserna M. J. Angebranndt and J. D. Winefordner Surface-enhanced Raman analysis of sulfa drugs on colloidal silver dispersion Anal. Chem.62 (1990) 689--693; DOI: 10.1021/ac00206a008.

  • P. Nagaraja K. R. Sunitha R. A. Vasantha and H. S. Yathirajan Iminodibenzyl as a novel coupling agent for the spectrophotometric determination of sulfonamide derivatives Eur. J. Pharm. Biopharm.53 (2002) 187--192; DOI:10.1016/S0939--6411(01)00235-1.

  • P. Nagaraja H. S. Yathirajan C. R. Raju R. A. Vasantha M. S. Hemantha Kumar and P. Nagendra 3-Aminophenol as a novel coupling agent for the spectrophotometric determination of sulfonamide derivatives Farmaco58 (2003) 1295--1300; DOI: 10.1016/S0014-827X (03) 00093-4.

  • S. Raghuveer I. R. K. Raju D. K. Vastu and C. M. R. Shrivastava Colorimetric determination of sulphamethoxazole in pharmaceutical preparations Indian Drugs30 (1993) 132--135.

  • A. S. Amin and M. M. Zarch Acetylacetone-formaldehyde reagent for the spectrophotometric determination of some sulfa drugs in pure and dosage forms Mikrochim. Acta.124 (1996) 227--233; DOI: 10.1007/BF01242820.

  • S. S. Sabry Enhanced spectrophotometry of sulfonamides with 2-acetylbutyrolactone derivatives Anal. Lett.39 (2006) 2591--2615; DOI: 10.1080/00032710600824748.

  • ICH Topic Q2(R1) Validation of Analytical Procedures: Text and Methodology (CPMP/ICH/281/95) acessed October 27 2006.

  • R. A. Day Jr. and A. L. Underwood Errors and the Treatment of Analytical Data in Quantitative Analysis 6th ed. Prentice-Hall of India New Delhi 1999 pp. 7--42.

  • A. M. Mohamed H. F. Askal and G. M. Saleh Use of p-benzoquinone for the spectrophotometric determination of certain sulphonamides J. Pharm. Biomed. Anal.9 (1991) 531--538; DOI: 10.1016/0731-7085(91) 80174-8.

  • G. R. Rao S. S. N. Murthy P. J. Rao and I. R. K. Raju Spectrophotometric determination of sulphacetamide sodium with ℴ-chloranil in dosage form Indian J. Pharm. Sci.50 (1988) 138--140.

  • A. G. Fogg and N. M. Fayad Spectrophotometric and differential pulse polarographic determination of sulphaguanidine by reaction with hypochlorite and phenol Anal. Chim. Acta.106 (1979) 365--367; DOI:10.1016/S0003-2670(01)85022-4.

  • P. Nagaraja H. S. Yathirajan K. R. Sunitha and R. A. Vasantha A new sensitive and rapid spectrophotometric method for the determination of sulfa drugs JAOAC Int.85 (2002) 869--874.

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