Hepatoprotective effect of the ethyl acetate extract of Teucrium polium L. against carbontetrachloride-induced hepatic injury in rats

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Hepatoprotective effect of the ethyl acetate extract of Teucrium polium L. against carbontetrachloride-induced hepatic injury in rats

Hepatoprotective activity of the ethyl acetate extract of Teucrium polium L. was investigated using rats with CCl4--induced liver damage. Specific biochemical parameters (glutathione peroxidase, superoxide dismutase, reduced glutathione and total antioxidative status) were estimated in blood and in liver homogenate. Lipid peroxidation in CCl4--intoxicated rats was evidenced by a marked increment in the levels of thiobarbituric acid reactive substances. Histopatological examinations of the liver were undertaken to monitor the liver status. Silymarin was used as a standard to compare the hepatoprotective activity of the extract. Some biochemical parameters in groups treated with the Teucrium polium extract at a dose of 25 mg kg-1, showed significantly different values than that of the CCl4--treated group. The liver biopsy of all experimental rat groups treated with the Teucrium polium ethyl acetate extract showed significant restoration of the normal histomorphological pattern of liver cells. The study substantiates the potential hepatoprotective activity of the ethyl acetate extract of Teucrium polium L.

Hepatoprotective effect of the ethyl acetate extract of Teucrium polium L. against carbontetrachloride-induced hepatic injury in rats

Hepatoprotektivno djelovanje etil-acetatnog ekstrakta biljke Teucrium polium L. ispitivano je na Štakorima čija je jetra oštećena tetraklormetanom. Specifični biokemijski parametri (glutation peroksidaza, superoksid dismutaza, reducirani glutation i ukupni antioksidativni status) procijenjeni su u krvi i homogenatu jetre. Lipidna peroksidacija u Štakora intoksiciranih tetraklormetanom dokazana je povećanjem koncentracije reaktivnih derivata tiobarbiturne kiseline. Stanje jetre procijenjeno je histopatološkim ispitivanjima. U ispitivanjima hepatoprotektivnog djelovanja ekstrakta Teucrium polium upotrebljen je silimarin kao standard. Neki biokemijski parametri u skupini životinja tretiranih ispitivanim ekstraktom u dozi od 25 mg kg-1 imali su značajno različite vrijednosti od skupine tretirane s CCl4. Biopsija jetre u svim ispitivanim životinjama tretiranim ekstraktom pokazala je uspostavu normalnih histomorfoloških uvjeta u stanicama, Što ukazuje na hepatoprotektivno djelovanje biljke Teucrium polium.

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