Our Experiences With B Cell Clonality Analysis Using Multiplex Pcr Amplification in Paraffin-Embedded Tissue
, and | May 30, 2014
Published Online: May 30, 2014
Page range: 5 - 13
Received: Feb 02, 2014
Accepted: Apr 22, 2014
DOI: https://doi.org/10.2478/acm-2014-0001
Keywords
© by L. Stanek
This article is distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
The clonal determination of B-cell lymphoproliferative diseases by immunoglobulin heavy chain (IgH) rearrangement by polymerase chain reaction (PCR) is widely used. In this study we report our experiences with B cell clonality analysis using multiplex PCR amplification followed by heteroduplex analysis, which was found to be essential for the efficient resolution of monoclonal bands within polyclonal backgrounds, in formalin-fixed paraffinembedded tissue.