Oxidative Damage to Proteins and Lipids During Ageing

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Oxidative Damage to Proteins and Lipids During Ageing

The present work is a theoretical study in the field of monitoring oxidative damage to proteins and lipids during ageing. The basic terminology and interactions are discussed as well as the sources of oxidants and their elimination through antioxidant protection. We focus on the effects of oxidative stress on the biomolecules (proteins, lipids and DNA), the role of mitochondria, antioxidants, physical activity and caloric restriction in relation to ageing. Previous research indicates the crucial role of mitochondria in the ageing process by their formation of oxidants, the accumulation of oxidative damage to mtDNA and other biomolecules leading to impairment of mitochondrial function, energy failure, apoptosis and necrosis. Although the role of oxidative stress in the ageing process is evident and well documented, the precise mechanisms of its relationships remain largely unknown. Further research is needed to clarify them and to show ways to slow down the ageing process.

  • Judge S, Leeuwenburgh C. Cardiac mitochondrial bioenergetics, oxidative stress, and aging. Am J Physiol Cell Physiol. 2007; 292(6): C1983-92

  • Harman D. Aging: a theory based on free radicals and radiation chemistry. J Gerontol. 1956; 2: 298-300

  • Dufour E, Larsson NG. Understanding aging: revealing order out of chaos. Biochim Biophys Acta. 2004; 1658(1-2): 122-32

  • Osiewacz HD. Role of mitochondria in ageing and age-related disease. Exp Gerontol. 2010; 45(7-8): 465

  • Cocco T, Sgobbo P, Clemente M, Lopriore B, Grattagliano I, Di Paola M, Villani G. Tissue-specific changes of mitochondrials functions in aged rats: effect of a long- term dietary treatment with N-acetylcysteine. Free Radic Biol Med. 2005; 38(6): 796-805

  • Kaplán P. Kardiovaskulárne ochorenia a voľné radikály. P+M Turany, 2010; 120 s.

  • Stadtman ER. Protein oxidation and aging. Free Radical Research. 2006; 40(12): 1250-8

  • Catalá A. Lipid peroxidation of membrane phospholipids generates hydroxy-alkenas and oxidized phospholipids active in physiological and/or pathological conditions. Chem Phys Lipids. 2009; 157(1): 1-11

  • Halliwell B, Gutteridge JMC. Free radicals in biology and medicine: Antioxidant defences. 3rd ed. New York: Oxford Univ Press; 1999. Halliwell B, Gutteridge JMC eds.

  • Sivoňová M, Tatarková Z, Ďuračková Z, Dobrota D, Lehotský J, Matáková T, Kaplán P. Relationship between antioxidant potential and oxidative damage to lipids, proteins and DNA in aged rats. Physiol. Res. 2007; 56(6): 757-64

  • Ďuračková Z. Some current insights into oxidative stress. Physiol Res. 2010; 59(4): 459-69

  • Harman D. The biologic clock: the mitochondria? J Am Geriatr Soc. 1972; 20(4): 145-7

  • Zorov DB, Juhaszova M, Sollott SJ. Mitochondrial ROS-induced ROS release: an update and review. Biochim Biophys Acta. 2006; 1757(5-6): 509-17

  • Romano AD, Serviddio G, de Matthaeis A, Bellanti F, Vendemiale G. Oxidative stress and aging. Nephrol. 2010; 23(Suppl. 15): S29-36

  • Gao L, Laude K, Cai H. Mitochondrial patophysiology, reactive oxygen species, and cardiovascular diseases. Vet Clin North Am Small Anim Pract. 2008; 38(1): 137-55

  • Trifunovic A, Larsson NG. Mitochondrial disfunction as a cause of ageing. J Intern Med. 2008; 263(2):167-78

  • Esterhazy D, King MS, Yakovlev G, Hirst J. Production of reactive oxygen species by complex I (NADH:ubiquinone oxidoreductase) from Escherichia coli and comparison to the enzyme from mitochondria. Biochemistry. 2008; 47(12): 3964-71

  • Zhang HM, Zhang Y, Zhang BX. The role of mitochondrial complex III in melatonin-induced ROS production in cultured mesangial cells. J Pineal Res. 2011; 50(1): 78-82.

  • Strkov AA, Fiskum G, Chinopoulos C, Lorenzo BJ, Browne SE, Patel MS, Beal MF. Mitochondrial alphaketoglutarate dehydrogenase complex generates reactive oxygen species. J Neurosci. 2004; 24(36): 7779-88

  • Wang W, Fang H, Groom L, Cheng A, Zhang W, Liu J, Wang X, Li K, Han P, Zheng M, Yin J, Mattson MP, Kao JP, Lakatta EG, Sheu SS, Ouyang K, Chen J, Dirksen RT, Cheng H. Superoxide flashes in single mitochondria. Cell. 2008; 134(2): 279-90

  • Babušíková E, Jesenák M, Račay P, Dobrota D, Kaplán P. Oxidative alternations in rat heart homogenate and mitochondria during ageing. Gen Physiol Biophys. 2008; 27(2): 115-20

  • Kaplán P, Tatarková Z, Račay P, Lehotský J, Pavlíková M, Dobrota D. Oxidative modifications of cardiac mitochondria and inhibition of cytochrome c oxidase activity by 4-hydroxynonenal. Redox Rep. 2007; 12(5): 211-8

  • Long J, Wang X, Gao H, Liu Z, Liu C, Miao M, Liu J. Malonaldehyde acts as a mitochondrial toxin: Inhibitory effects on respiratory function and enzyme activities in isolated rat liver mitochondria. Life Sci. 2006; 79(15): 1466-7

  • Kumaran S, Subathra M, Balu M, Panneerselvam C. Age-associated decreased activities of mitochondrial electron transport chain complexes in heart and skeletal muscle: role of L-carnitine. Chem Biol Interact. 2004; 148(1-2): 11-8

  • Tatarková Z, Kuka S, Račay P, Lehotský J, Dobrota D, Mištuna D, Kaplán P. Effects of aging on activities of mitochondrial electron transport chain complexes and oxidative damage in rat heart. Physiol Res. 2011; 60(2): 281-9

  • Davies SM, Poljak A, Duncan MW, Smythe GA, Murphy MP. Measurements of protein carbonyls, orthoand meta-tyrosine and oxidative phosphorylation complex activity in mitochondria from young and old rats. Free Radic Biol Med. 2001; 31(2): 181-90

  • Preston CC, Oberlin AS, Holmuhamedov EL, Gupta A, Sagar S, Syed RH, Siddiqui SA, Raghavakaimal S, Terzic A, Jahangir A. Aging-induced alterations in gene transcripts and functional activity of mitochondrial oxidative phosphorylation complexes in the heart. Mech Ageing Dev. 2008; 129(6): 304-12

  • Choksi KB, Papaconstantinou J. Age-related alterations in oxidatively damaged proteins of mouse heart mitochondrial electron transport chain complexes. Free Radic Biol Med. 2008; 44(10): 1795-805

  • Petrosillo G, Matera M, Moro N, Ruggerio FM, Paradies G. Mitochondrial complex I dysfunction in rat heart with aging: critical role of reactive oxygen species and cardiolipin. Free Radic Biol Med. 2009; 46(1): 88-94

  • Gómez LA, Monette JS, Chavez JD, Maier CS, Hagen TM. Supercomplexes of the mitochondrial electron transport chain decline in the aging rat heart. Arch Biochem Biophys. 2009; 490: 30-35

  • Stadtman ER. Role of oxidant species in aging. Curr Med Chem. 2004; 11(9): 1105-12

  • Lee S, Park Y, Zuidema MY, Hannink M, Zhang C. Effects of interventions on oxidative stress and inflammation of cardiovascular diseases. World J Cardiol. 2011; 3(1): 18-24

  • Singh M, Nam DT, Arsenault M, Ramassamy C. Role of by-products of lipid oxidation in Alzheimer's disease brain: a focus on acrolein. J Alzheimers Dis. 2010; 21(3): 741-56

  • Fernández-Checa JC, Fernández A, Morales A, Marí M, García-Ruiz C, Colell A. Oxidative stress and altered mitochondrial function in neurodegenerative diseases: lessons from mouse models. CNS Neurol Disord Drug Targets. 2010; 9(4): 439-54

  • Palardó FV, Lloret A, Lebel M, d Ischia M, Cogger VC, Le Couteur DG, Gadaleta MN, Castello G, Pagano G. Mitochondrial dysfunction in some oxidative stress-related genetic diseases: Ataxia-Telangiectasia, Down Syndrome, Fanconi Anaemia and Werner Syndrome. Biogerontology. 2010; 11(4): 401-19

  • Shoham A, Hadziahmetovic M, Dunaief JL, Mydlarski MB, Schipper HM. Oxidative stress in diseases of human cornea. Free Radic Biol Med. 2008; 45(8): 1047-55

  • Moustafa AH, Ali M, Mohamed TM, Abdou HI. Oxidative stress and thyroid hormones in patients with liver diseases. Eur J Intern Med. 2009; 20(7): 703-8

  • Giustarini D, Dalle-Donne I, Tsikas D, Rossi R. Oxidative stress and human diseases: Origin, link, measurement, mechanisms, and biomarkers. Crit Rev Clin Lab Sci. 2009; 46(5-6): 241-81

  • Ciechanover A. The ubiquitin proteolytic system: from an idea to the patient bed. Proc Am Thorac Soc. 2006; 3(1): 21-31

  • Farout L, Friguet B. Proteasome Function in Aging and Oxidative Stress: Implications in Protein Maintenance Failure. Antioxid Redox Signal. 2006; 8(1-2): 205-16

  • Jung T, Bader N, Grune T. Lipofuscin: formation, distribution, and metabolic consequences. Ann N Y Acad Sci. 2007; 1119: 97-111

  • Semba RD, Nicklett EJ, Ferrucci L. Does accumulation of advanced glycation end products contribute to the aging phenotype? J Gerontol A Biol Sci Med Sci. 2010; 65(9): 963-75

  • Savitha S, Panneerselvam C. Mitochondrial membrane damage during aging process in rat heart: potential efficacy of L-carnitine and DL alpha lipoic acid. Mech. Ageing Dev. 2006; 27(4): 349-55

  • Savitha S, Panneerselvam C. Mitigation of age-dependent oxidative damage to DNA in rat heart by carnitine and lipoic acid. Mech Ageing Dev. 2007; 128(2): 206-12

  • Tamilselvan J, Jayaraman G, Sivarajan K, Panneerselvam C. Age-dependent upregulation of p53 and cytochrome c release and susceptibility to apoptosis in skeletal muscle fiber of aged rats: role of carnitine and lipoic acid. Free Radic Biol Med. 2007; 43(12): 1656-69

  • Sudheesh NP, Ajith TA, Janardhanan KK, Krishnan CV. Palladium alpha-lipoic acid complex formulation enhances activities of Krebs cycle dehydrogenases and respiratory complexes I-IV in the heart of aged rats. Food Chem Toxicol. 2009; 47(8): 2124-8

  • Salmon AB, Richardson A, Pérez VI. Update on the oxidative stress theory of aging: Does oxidative stress play a role in aging or healthy aging? Free Radic Biol Med. 2010; 48(5): 642-55

  • Judge S, Jang YM, Smith A, Selman C, Phillips T, Speakman JR, Hagen T, Leeuwenburgh C. Exercise by lifelong voluntary wheel running reduces subsarcolemmal and interfibrillar mitochondrial hydrogen peroxide production in the rat heart. Am J Physiol Regul Integr Comp Physiol. 2005; 289(6): R1564-72

  • Navarro A, Gomez C, López-Cepero JM, Boveris A. Beneficial effects of moderate exercise on mice aging: survival, behavior, oxidative stress, and mitochondrial electron transfer. Am J Physiol Regul Integr Comp Physiol. 2004; 286(3): R505-11

  • Ascensão A, Ferreira R, Magalhães J. Exercise-induced cardioprotection - biochemical, morphological and functional evidence in whole tissue and isolated mitochondria. Int J Cardiol. 2007; 117(1): 16-30

  • Sinclair DA. Toward a theory of caloric restriction and longevity regulation. Mech Ageing Dev. 2005; 126: 987-1002

  • Li Y, Daniel M, Tollefsbol T. Epigenetic regulation of caloric restriction in aging. BMC Med. 2011; 9(1): 98 [Epub ahead of print]

  • Wakeling LA, Ions LJ, Ford D. Could Sirt1-mediated epigenetic effects contribute to the longevity response to dietary restriction and be mimicked by other dietary interventions? Age (Dordr). 2009; 31(4):327-41

Acta Medica Martiniana

The Journal of Comenius University in Bratislava

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