Effects of Priming with Ascorbic Acid, L-Cystein and Triacontanol on Germination of Rapeseed (Brassica napus L.)

Katalin Molnár 1 , 3 , Béla Biró-Janka 1 , Imre-István Nyárádi 1 , László Fodorpataki 2 , Bernadett-Enikő Varga 1 , János Bálint 1 , and Marcel-Matei Duda 3
  • 1 Department of Horticulture, Faculty of Technical and Human Sciences, University of Transylvania, Cluj-Napoca, Romania
  • 2 Department Department of Taxonomy and Ecology, Faculty of Biology and Geology, Babeș-Bolyai University, Cluj-Napoca, Romania
  • 3 Faculty of Agriculture, University of Agriculture and Veterinary Medicine, Cluj-Napoca, Romania


The germination of seed batches of two rapeseed (Brassica napus L.) hybrids (‘Hybrirock’ and ‘Factor’) were investigated in response to priming in aqueous solutions of ascorbic acid (10 mM), L-cysteine (10 mM) and triacontanol (1 μM), respectively tap water (as control treatment). Investigations were focused on seed quality parameters, such as germination percentage, mean germination time, germination index, uniformity of germination, and seedling shoot and root growth. Germinated seeds were counted every 24 hours for a period of seven days, recording the final germination percentage (FGP), mean germination time (MGT), the coefficient of velocity of germination (CVG), germination rate index (GRI), germination index (GI), uncertanty (U), and synchrony (Z). In a separate trial fresh shoot- and root length, respectively the dry weight of the shoots and roots were also determined after a 14 day period. Germination tests were performed to examine the quality of seeds in response to different priming treatments. The results demonstrated that priming improved the germination parameters of seeds of both hybrids. Furthermore, the use of ascorbic acid, L-cysteine and triacontanol also enhanced seedling growth. The results may be used to rank seed lots by vigor, and decisions can be made regarding planting potential of each seed lot.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Al-Mudaris M (1998) Notes on various parameters recording the speed of seed germination. In: Der Tropenlandwirt - Journal of Agriculture in the Tropics and Subtropics, Vol 99, No2, pp 147‒154.

  • 2. Amirmoradi S, Feizi H (2017) Can mean germination time predict seed vigor of canola (Brassica napus L.) seed lots? Acta Agrobot. 70(4):1729. https://doi.org/10.5586/aa.1729

  • 3. Ansari O, Sharif-Zadeh F, Moradi A, Azadi MS, Younesi E (2013) Heat shock treatment can improve some seed germination indexes and enzyme activity in primed seeds with gibberellin of mountain rye (Secale montanum) under accelerated aging conditions. Cercet. Agron. Moldova 156 (4):21‒30.

  • 4. Aravind J, Vimala Devi S, Radhamani J, Jacob S R, and Kalyani Srinivasan (2020) germinationmetrics: Seed Germination Indices and Curve Fitting. R package version https://github.com/aravindj/germinationmetricshttps://cran.rproject.org/package=germinationmetrics

  • 5. Ashraf MA, Akbar A, Askari SH, Iqbal M, Rasheed R, Hussain I (2018) Recent Advances in Abiotic Stress Tolerance of Plants Through Chemical Priming: An Overview. In: Rakshit A., Singh H. (eds) Avances in Seed Priming. Springer, Singapore. https://doi.org/10.1007/978-981-13-0032-5_4

  • 6. Bhuiyan TF, Ahamed KU, Nahar K, Al Mahmud J, Bhuyan MB, Anee TI, Fujita M, Hasanuzzaman M (2019). Mitigation of PEG-induced drought stress in rapeseed (Brassica rapa L.) by exogenous application of osmolytes. Biocatal. Agric. Biotechnol. 20:1–10. https://doi.org/10.1016/j.bcab.2019.101197

  • 7. Saha B, Chowardhara B, Kar S, Devi SS, Awasthi JP, Moulick D, Tanti B, Panda SK (2019) Advances in Heavy Metal-Induced Stress Alleviation with Respect to Exogenous Amendments in Crop Plants. In: Hasanuzzaman M., Fotopoulos V. (eds) Priming and Pretreatment of Seeds and Seedlings. Springer, Singapore. https://doi.org/10.1007/978-981-13-8625-1_15

  • 8. Deviah SP, Pan X, Hong Y, Roth M, Welti R, Wang X (2007) Enhancing seed quality and viability by suppressing phospholipase D in Arabidopsis. The Plant J. 50: 950‒957.

  • 9. Dutta P (2018) Seed Priming: New Vistas and Contemporary Perspectives. In: Rakshit A., Singh H. (eds) Advances in Seed Priming. Springer, Singapore. https://doi.org/10.1007/978-981-13-0032-5_1

  • 10. Jisha KC, Vijayakumari K, Puthur JT (2013) Seed priming for abiotic stress tolerance: an overview. Acta Physiol Plant 35(5):1381–1396. https://doi.org/10.1007/s11738-012-1186-5

  • 11. Kader M. A (2005) A comparison of seed germination calculation formulae and the associated interpretation of resulting data. Journal & Proceedings of the Royal Society of New South Wales 138:65–75.

  • 12. Kandil AAEN, Sharief AES, Botabaah AKD (2018) Effect of antioxidants and salinity stress on seedling parameters of some wheat cultivars. Res. J. Seed Sci. 11:12–21.

  • 13. Kandil AA, Sharief AE, Kasim MF (2016) Germination characters as affected by seed priming of some safflower cultivars under salinity stress. Int. J. Agron. Agric. Res. 9:65–80.

  • 14. Khan NA, Khan MI, Asgher M, Fatma M, Masood A, Syeed S (2014) Salinity tolerance in plants: revisiting the role of sulfur metabolites. J Plant Biochem Physiol 2(120):2.

  • 15. Nesi N, Delourme R, Bregeon M, Falentin C, Renard M (2008) Genetic and molecular approaches to improve nutritional value of Brassica napus L. seed. C R Biol 331:763–771.

  • 16. Ozbay N (2018) Studies on Seed Priming in Pepper (Capsicum annuum L.). In: Rakshit A., Singh H. (eds) Advances in Seed Priming. Springer, Singapore. https://doi.org/10.1007/978-981-13-0032-5_12

  • 17. Ranal MA, Garcia de Santana D, Ferreira WR, Mendes-Rodrigues C (2009) Calculating germination measurements and organizing spreadsheets. In: Revista Brasiliera Botanica 32, pp 849–855.

  • 18. Razaji A, Farzanian M, Sayfzadeh S (2014) The effects of seed priming by ascorbic acid on some morphological and biochemical aspects of rapeseed (Brassica napus L.) under drought stress condition. Int J Biosci 4(1):432–442.

  • 19. Reddy CS, Smith JD (1978) Effects of delayed post treatment of gamma-irradiated seed with cysteine on the growth of Sorghum bicolor seedlings. Environ Exp Bot 18(4):241–243. https://doi.org/10.1016/0098-8472(78)90050-3

  • 20. Seiadat SA, Moosavi A, Sharafizadeh M (2012) Effect of seed priming on antioxidant activity and germination characteristics of Maize seeds under different aging treatments. Research Journals of Seed Scienc 5(2):51–62.

  • 21. Tantos Á, Mészáros A, Kissimon J, Horváth G, Farkas T (1999) The effect of triacontanol on micropropagation of balm, Melissa officinalis L. Plant Cell Rep. 19:88–91.

  • 22. Walters, C (1998) Understanding the mechanisms and kinetics of seed aging. Seed. Sci. Res. 8:223–244.

  • 23. *** SR (Standard Român) 1634, (1999) Seeds for sowing. Germination test


Journal + Issues