Comparative Histological Evaluation of the Fruit of Ribes nigrum and Ribes rubrum

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Abstract

To complete the wide range of studies on chemical composition and pharmacological potential of the fruit of Ribes nigrum and Ribes rubrum, this work aimed to perform a comparative morpho-anatomical characterization of the fruits of both species and complete previously records of black currant as frequently studied species. Microslides of fruits were made with rotation microtome, and then cross sections were stained with toluidine blue. Documentation and evaluation were carried out in microphotos. In R. rubrum, the epidermis cells are ovoid to round, while those of R. nigrum are rectangular to almost round. Under the epidermis, in both fruits, there are two layers of oval hypodermal cells. Underneath the hypodermal layers, parenchyma cells of different size with thin walls and scattered collateral closed bundles were detected. The seeds are embedded in a gelatinous sheath having large thin-walled cells. Endocarp separates the arillar tissue from parenchyma cells, in addition, a gap can be found among these layers, endocarp, arillar tissue, and seeds. The seed coat consists of sclerenchyma cells in both species. In conclusion, histological differences and similarities were described in the fruits of the selected Ribes species, highlighted the first morpho-anatomical description of the berry of Ribes rubrum.

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  • 1. Békési-Kallenberger H Horváth GY Bencsik T Balázs Vl Filep R Papp N (2016) Comparative Histological and Phytochemical Study of Fallopia species. Natural Product Communications 11:251–254.

  • 2. Bishayee A Háznagy-Radnai E Mbimba T Sipos P Morazzoni P Darvesh AS Bhatia D Hohmann J (2010) Anthocyanin-rich black currant extract suppresses the growth of human hepatocellular carcinoma cells. Natural Product Communications 5:1613–1618.

  • 3. Ciocarlan V (2000) Flora ilustrată a României Editura Ceres Bucureşti

  • 4. Corner EJH (1976) The seeds of dicotyledons. Cambridge University Press Cambridge

  • 5. Cyboran S Bonarska-Kujawa D Pruchnik H Zyłka R Oszmiański J Kleszczyńska H (2014) Phenolic content and biological activity of extracts of black currant fruit and leaves. Food Research International 65:47–58. doi: 10.1016/j.foodres.2014.05.037.

  • 6. Da Silva Pinto M Kwon Yi Apostolidis E Lajolo FM Genovese M Shetty K (2010) Evaluation of red currants (Ribes rubrum L.) black currants (Ribes nigrum L.) red and green gooseberries (Ribes uva-crispa) for potential management of type 2 diabetes and hypertension using in vitro models. Journal of Food Biochemistry 34:639–660. doi: 10.1111/j.1745-4514.2009.00305.x

  • 7. EU-NOMEN (n.d.) http://www.eu-nomen.eu/portal/taxon.php?GUID=3734D1D0-9A3C-41FC-BCB9-20279F1C1502 (accessed 18 sept. 2018).

  • 8. Gavrilova V Kajdžanoska M Gjamovski V Stefova M (2011) Separation characterization and quantification of phenolic compounds in blueberries and red and black currants by HPLC-DAD-ESI-MSn. Journal of Agricultural and Food Chemistry 59(8):4009–4018. doi: 10.1021/jf104565y

  • 9. Giongo L Poncetta P Loretti P Costa F (2013) Texture profiling of blueberries (Vaccinium spp.) during fruit development ripening and storage. Postharvest Biology and Technology 76:34–39. doi: 10.1016/j.postharvbio.2012.09.004

  • 10. Glidewell S Williamson B Duncan GH Chudek J Hunter G (1999) The development of black currant fruit from flower to maturity: A comparative study by 3D nuclear magnetic resonance (NMR) micro-imaging and conventional histology. New Phytologist 141(1):85–98. doi: 10.1046/j.1469-8137.1999.00319.x

  • 11. Goffman F Galetti S (2001) Gamma-linolenic acid and tocopherol contents in the seed oil of 47 accessions from several Ribes species. Journal of Agricultural and Food Chemistry 49(1):349–354. doi: 10.1021/jf0006729

  • 12. Häkkinen SH Kärenlampi SO Heinonen IM Mykkänen HM Törronen AR (1999) Content of the flavonols quercetin myricetin and kaempferol in 25 edible berries. Journal of Agricultural and Food Chemistry 47(6):2274–2279. doi: 10.1021/jf9811065

  • 13. Kendir G Güvenç A Acar A Çeter T Pinar NM (2015) Fruits seeds and pollen morphology of Turkish Ribes L. (Grossulariaceae). Plant Systematics and Evolution 301(1):185–199. doi: 10.1007/s00606-014-1064-2

  • 14. Knekt P Järvinen R Seppänen R Hellövaara M Teppo L Pukkala E Aromaa A (1997) Dietary flavonoids and the risk of lung cancer and other malignant neoplasms. American Journal of Epidemiology 146(3):223–30. doi: 10.1093/oxfordjournals.aje.a009257

  • 15. Leskinen H Suomela Kallio H (2009) Effect of latitude and weather conditions on the regioisomer compositions of α-and γ-linolenoyldilinoleoylglycerol in currant seed oils. Journal of Agricultural and Food Chemistry. 57(9):3920–3926. doi: 10.1021/jf900068b

  • 16. Liu P Kallio H Yang B (2014) Flavonol glycosides and other phenolic compounds in buds and leaves of different varieties of black currant (Ribes nigrum L.) and changes during growing season. Food Chemistry 160:180–189. doi: 10.1016/j.foodchem.2014.03.056

  • 17. Marhold K (2011) Grossulariaceae. Euro+Med Plantbase. http://ww2.bgbm.org/euroPlusMed/PTaxonDetail.asp?UUID=3734D1D0-9A3C-41FC-BCB9-20279F1C1502 (accessed 18 september 2018).

  • 18. Metcalfe CR Chalk L (1957) Anatomy of the dicotyledons vol I. Oxford At The Clarendon Press

  • 19. Mikulic-Petkovsek M Rescic J Schmitzer V Stampar F Slatnar A Koron D Veberic R (2015) Changes in fruit quality parameters of four Ribes species during ripening. Food Chemistry 173:363–374. doi: 10.1016/j.foodchem.2014.10.011

  • 20. Mikulic-Petkovsek M Schmitzer V Slatnar A Stampar F Veberic R (2012) Composition of sugars organic acids and total phenolics in 25 wild or cultivated berry species. Journal of Food Science 77(10):1–7. doi: 10.1111/j.1750-3841.2012.02896.x

  • 21. Milivojevic J Slatnar A Mikulic-Petkovsek M Stampar F Nikolic M Veberic R (2012) The influence of early yield on the accumulation of major taste and health-related compounds in black and red currant cultivars (Ribes spp.). Journal of Agricultural and Food Chemistry 60(10):2682–2691. doi: 10.1021/jf204627m

  • 22. Nour V Trandafir I Cosmulescu S (2014) Antioxidant capacity phenolic compounds and minerals content of blackcurrant (Ribes nigrum L.) leaves as influenced by harvesting date and extraction method. Industrial Crops and Products 53:133–139. doi: 10.1016/j.indcrop.2013.12.022

  • 23. Papp N Csete S Farkas Á (2013) Comparative ecomorphology of the cyathial nectaries in eight European Euphorbia species. Acta Biologica Hungarica 64(1):45–59. 10.1556/ABiol.64.2013.1.5

  • 24. Saladie M Matas AJ Isaacson T Jenks MA Goodwin SM Niklas KJ Xiaolin R Labavitch JM Shackel KA Fernie AR Lytovchenko A O’neill MA Watkins CB Rose JKC (2007) A reevaluation of the key factors that influence tomato fruit softening and integrity. Plant Physiology 144(2):1012–1028. doi: 10.1104/pp.107.097477

  • 25. Sasaki T Li W Zaike S Asada Y Li Q Ma F Zhang Q Koike K (2013) Antioxidant lignoids from leaves of Ribes nigrum. Phytochemistry 95:333–340. doi: 10.1016/j.phytochem.2013.07.022

  • 26. Šavikin K Zdunić G Janković T Tasić S Menković N Stević T Đorđević B (2009) Phenolic content and radical scavenging capacity of berries and related jams from certificated area in Serbia. Plant Foods for Human Nutrition 64(3):212–217. doi: 10.1007/s11130-009-0123-2

  • 27. Schweingruber FH Börner A Schulze ED (2011) Atlas of Stem Anatomy in Herbs Shrubs and Trees vol 1 Springer-Verlag Berlin Heidelberg

  • 28. Da Silva Pinto M Kwon Y Apostolidis E Lajolo FM Genovese MI Shetty K (2010) Evaluation of red currants (Ribes Rubrum L.) black currants (Ribes Nigrum L.) red and green gooseberries (Ribes Uva-Crispa) for potential management of type 2 diabetes and hypertension using in vitro models. Journal of Food Biochemistry 34(3):639–660. doi: 10.1111/j.1745-4514.2009.00305.x

  • 29. Slavin JL Lloyd B (2012) Health benefits of fruits and vegetables. Advances in nutrition (Bethesda Md.) 3(4):506–16. doi: 10.3945/an.112.002154

  • 30. The Plant List http://www.theplantlist.org/1.1/browse/A/Grossulariaceae/ (accessed 9.18.18).

  • 31. Traitler H Winter H Richli U Ingenbleek Y (1984) Characterization of gamma-linolenic acid in Ribes seed. Lipids 19(12):923–928. doi: 10.1007/BF02534727

  • 32. USDA. (n.d.). PlantsDatabase. https://plants.usda.gov/core/profile?symbol=RIBES (accessed 9.18.18).

  • 33. Vagiri M Ekholm A Öberg E Johansson E Andersson SC Rumpunen K (2013) Phenols and ascorbic acid in black currants (Ribes nigrum L.): Variation due to genotype location and year. Journal of Agricultural and Food Chemistry 61(39):9298–9306. doi: 10.1021/jf402891s

  • 34. Vuorinen Al Kalpio M Linderborg KM Hoppula KB Karhu ST Yang B Kallio HP (2016) Triacylglycerol biosynthesis in developing Ribes nigrum and Ribes rubrum seeds from gene expression to oil composition. Food Chemistry 196:976—987. doi: 10.1016/j.foodchem.2015.10.010

  • 35. Williamson B Goodman BA Chudek JA (1993) The structure of mature gooseberry (Ribes grossularia) fruits revealed noninvasively by NMR microscopy. Micron 24(4):377–383. doi: 10.1016/0968-4328(93)90003-J

  • 36. Wojdylo A Oszmianski O Milczarek M Wyetrzyk J (2013) Phenolic profile antioxidant and antiproliferative activity of black and red currants (Ribes spp.) from organic and conventional cultivation. International Journal of Food Science and Technology 48:715–726. doi: 10.1111/ijfs.12019

  • 37. Woznicki Tl Sønsteby A Aaby K Martinsen BK Heide OM Wold AB Remberg SF (2017) Ascorbate pool sugars and organic acids in black currant (Ribes nigrum L.) berries are strongly influenced by genotype and post-flowering temperature. Journal of the Science of Food and Agriculture 97(4):1302–1309. doi: 10.1002/jsfa.7864

  • 38. Wrońska-Pilarek D (2002) Seed morphology of the native species of the genus Ribes L. Part 2. The characteristics of individual species. Acta Societatis Botanicorum Poloniae 71(1):5–16. doi: 10.5586/asbp.2002.001

  • 39. Wrońska-Pilarek D (2001) Seed morphology of the polish native species of the genus Ribes L. Part 1. General characteristic. Acta Societatis Botanicorum Poloniae 70(4):281–289. doi: 10.5586/asbp.2001.036

  • 40. Zágoni E (2005) A feketeribiszke. Alutus kiadó Miercurea Ciuc

  • 41. Zdunić G Šavikin K Pljevljakušić D Djordjević B (2016) Black (Ribes nigrum L.) and red currant (Ribes rubrum L.) cultivars in: Simmonds M. Preedy V. (Eds.) Nutritional Composition of Fruit Cultivars. Academic Press pp. 101–106.

  • 42. Zheng J Yang B Ruusunen V Laaksonen O Tahvonen R Hellsten J Kallio H (2012) Compositional differences of phenolic compounds between blackcurrant (Ribes nigrum L.) cultivars and their response to latitude and weather conditions. Journal of Agricultural and Food Chemistry 60(26):6581–6593. doi: 10.1021/jf3012739.

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