Recent studies have shown that vesicular-arbuscular mycorrhizae stimulate plant growth in case of Artemisia annua plants. According to these studies mycorrhization can enhance plant height and biomasses, shoot branching and inter-nodal length, foliar glandular hair density, and nutrient status of shoots and leafs. Contradictory data were obtained in case of leaf chlorophyll content and photosynthetic rate. The effects of vesicular-arbuscular mycorrhizae on roots, shoots and leafs anatomy of A. annua have not been studied yet. The aim of this paper was to compare the microscopic characteristics of the vegetative organs from the Artemisia annua plants treated with vesicular-arbuscular mycorrhizae, with those from the control plants. Rhizophagus irregularis influenced the development of vascular tissues in root and stem of Artemisia plants by increasing their surface in the organs. Mycorrhization also reduced the percentage of lignification in the cortex of the root, increased the percentage of palisade parenchyma in leaf and had a positive effect on foliar glandular hair density. Further investigations are necessary to find out the role of these histo-anatomic alterations in the growth and development of Artemisia plants.
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1. Adolfsson L Solymosi K Andersson MX Keresztes Á Uddling J Schoefs B et al. (2015) Mycorrhiza Symbiosis Increases the Surface for Sunlight Capture in Medicago truncatula for Better Photosynthetic Production. PLoS ONE 10(1): e0115314. doi:10.1371/journal.pone.0115314
2. Chaudhary L V Kapoor R Bhatnagar K A (2008) Effectiveness of two arbuscular mycorrhizal fungi on concentrations of essential oil and artemisinin in three accessions of Artemisia annua L. Appl. Soil Ecol. 40 174–181. doi: 10.1016/j.apsoil.2008.04.003
3. Domokos E Jakab-Farkas L Darkó B Bíró-Janka B Mara Gy Albert Cs Balog A (2018) Increase in Artemisia annua plant biomass content and guaiacol peroxidase activity using the arbuscular mycorrhizal fungus Rhizophagus irregularis. Front Plant Sci. 9:478. doi: 10.3389/fpls.2018.00478
4. Fortin S Melchert V (2015) Effect of mycorrhizae on Artemisia annua. Worcester Polytechnic Institute.
5. Giri B (2017) Mycorrhizal fungus Rhizophagus fasciculatus promotes artemisinin accumulation in Artemisia annua. In Paper Presented at the Tropentag Future Agriculture: Socio-Ecological Transitions and Bio-Cultural Shifts Bonn.
6. Hammer Ø Harper D A T Ryan P D (2001). PAST: Paleontological statistics software package for education and data analysis. Palaeontologia Electronica 4(1): 9pp. http://palaeo-electronica.org/2001_1/past/issue1_01.htm
7. Huang J H Tan J F Jie H K Zeng R S (2011) Effects of inoculating arbuscular mycorrhizal fungi on Artemisia annua growth and its officinal components. Yingyong Shengtai Xuebao 22(6):1443–1449.
8. Ivănescu B Miron A Lungu C (2015) Histo-anatomy of vegetative organs of some Artemisia species. The Medical-Surgical Journal 119(3):917–924.
9. Kapoor R Chaudhary V Bhatnagar AK (2007) Effects of arbuscular mycorrhiza and phosphorus application on artemisinin concentration in Artemisia annua L. Mycorrhiza 17:581–587. doi: 10.1007/s00572-007-0135-4
10. Mandal S Upadhyay S Wajid S Ram M Jain D C Singh V P Abdin M Z Kapoor R (2015) Arbuscular mycorrhiza increase artemisinin accumulation in Artemisia annua by higher expression of key biosynthesis genes via enhanced jasmonic acid levels. Mycorrhiza. 25(5):345-57. doi: 10.1007/s00572-014-0614-3
11. Rapparini F Llusia J Penuelas J (2008) Effect of arbuscular mycorrhizal (AM) colonization on terpene emission and content of Artemisia annua L. Plant Biol 10:108–122. doi: 10.1055/s-2007-964963
12. Tan W D Shen M J Qiu H J Zeng F L Huang J H Huang R S Luo W G Liu Y X (2013) Effects of different phosphorus treatments on Arbuscular mycorrhizal formation growth and artemisinin content of Artemisia annua. Journal of Southern Agriculture 44(8):1303–1307.
13. Tanase C Silvia O Domokos E (2017) Botanică farmaceutică-Îndrumător de lucrări practice Vol. 3. University Press Târgu Mureș
14. Tu Y (2017) From Artemisia annua L. to artemisinins: the discovery and developement of artemisinins and antimalarial agents Academic Press Cambridge
15. Zheng H Z Cui C L Zhang Y T Wang D Jing Y Kim K Y (2005) Active changes of lignification-related enzymes in pepper response to Glomus intraradices and/or Phytophthora capsici. J Zhejiang Univ Sci B. 6(8):778–86.