Increased protein excretion, including albumin, by children of patients with urolithiasis
Article Category: Brief communication (Original)
Published Online: Jan 31, 2017
Page range: 401 - 408
DOI: https://doi.org/10.5372/1905-7415.0903.410
Keywords
© 2015 Thasinas Dissayabutra, Jakkapan Rattanapan, Nuttiya Kalpongnukul, Supawadee Mingmongkol, Wattanachai Ungchareonwattana, Chanchai Boonla, Piyaratana Tosukhowong
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Background
Patients with urolithiasis usually have increased urinary protein excretion.
Objectives
To compare the urinary protein, including albumin, excretion by patients with urolithiasis and their children, and identify the urinary proteins that are excreted more than they are by the normal population.
Materials and Methods
We recruited 28 patients with urolithiasis after stone removal (G1) and their nonstone-forming children (G2), and 30 healthy volunteers who lived in the same region (G3) and their children (G4). Medical history and 24 h urine were analyzed. Total urinary proteins and albumin were measured, and the urine proteome analyzed by two-dimensional SDS–polyacrylamide gel electrophoresis and mass spectrophotometry.
Results
Age, sex, body mass index, and amount of smoking and alcohol drinking were not different between G1 and G3, or G2 and G4. G1 patients had more prevalent underlying diseases than participants in the other groups. Urinary protein and albumin levels were highest in G1 participants, and were higher in G2 compared with G4. Preliminary proteomics showed elevated urinary Tamm–Horsfall protein, albumin, κ- and λ-2 light chain immunoglobulin excretion.
Conclusions
Patients with urolithiasis and their children had elevated excretion of urinary protein, including albumin, compared with the normal population, even though the levels were not clinically important. Leakage of these proteins suggest a tubular cell reabsorption defect that might associate with the pathogenesis of stone formation.