Sequence analysis of Plasmodium falciparum SERCA-type pfATPase6 in field isolates collected along Thai-Cambodian border
Article Category: Brief communication (Original)
Published Online: Jan 31, 2017
Page range: 343 - 351
DOI: https://doi.org/10.5372/1905-7415.0903.403
Keywords
© 2015 Wiriya Rutvisuttinunt, Kurt E. Schaecher, Harald Noedl, Chan Thap Lon, Stuart Tyner, Charlotte A. Lanteri, Krisada Jongsakul, Delia Bethell, Bryan L. Smith, David L. Saunders, Mark M. Fukuda
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
Background
Polymorphisms in the P. falciparum gene for ATPase6 (
Objective
To investigate the specific regions of
Methods
We examined polymorphisms in
Results
The majority of DNA sequences coding for the proposed artesunate binding pocket (M3, M5, and M7 helices) and the regions around Ser769 were conserved in parasite populations collected from patients in both study sites, regardless of clinical outcome.
Conclusions
The previously proposed areas of pfATPase6 did not appear to vary based on clinical outcome in a large number of patients from Southeast Asia, suggesting these regions are unlikely to be useful as molecular markers of resistance in clinical specimens from the Southeast Asian region.