Antimicrobial susceptibility, plasmid profiles, and RAPD-PCR typing of Acinetobacter bacteria

Open access

Abstract

Background: Multiple-drug resistant Acinetobacter have widely spread in the last decades imposing a serious nosocomial source of infection. Nevertheless, little knowledge was gaimed on tracing the development of antibiotic resistance in Acinetobacter species. Objectives: Explore Acinetobacter spp. via antimicrobial susceptibility, plasmid profiles, and random amplified polymorphism DNA polymerase chain reaction (RAPD-PCR) typing. Methods: One hundred twelve Acinetobacter isolates (including 66 A. baumannii and 46 non-Acinetobacter baumannii strains) were obtained from three university hospitals. The source of infection of these isolates included blood, urine, wound, and respiratory tract. Their susceptibilities to 17 antibiotics were tested and then all Acinetobacter isolates were typed by plasmid analysis and RAPD-PCR method. Results: A. baumannii isolates revealed nine different patterns of antibiotic resistance. Of those, non- A. baumannii, were associated with plasmid and RAPD-PCR typings (p <0.05). A. baumannii was more resistant to multiple antibiotics than non-A. baumannii (p <0.05). Seven different plasmid profiles were observed among 112 Acinetobacter isolates. Plasmids were found in 107 (95.5%) of the 112 isolates. Unlike in RAPD-PCR typing, there was no difference between the type of Acinetobacter, A. or non-A. baumannii strains and plasmid profiles (p >0.05). By RAPD-PCR, six profiles were found for each A. and non-A. baumannii strains. The pattern 6 was the most common pattern among the isolates. Both plasmid and RAPD-PCR typing showed no association between plasmid profiling and site of infection (p >0.05). Conclusion: There is a wide spread of multi-drug resistant Acinetobacter spp., particularly A. baumannii, in the Middle East region that can be traced efficiently by plasmid and genotyping typing of Acinetobacter. More care should be taken for tracing the development of antimicrobial resistance of Acinetobacter using precise molecular typing techniques.

If the inline PDF is not rendering correctly, you can download the PDF file here.

  • 1. Lautenbach E Synnestvedt M Weiner MG Bilker WB Vo L Schein J et al. Epidemiology and impact of imipenem resistance in Acinetobacter baumannii. Infect Control Hosp Epidemiol. 2009 30:1186-92.

  • 2. Seifert H Boullion B Schulze A Pulverer G. Plasmid DNA profiles of Acinetobacter baumannii: clinical application in a complex endemic setting. Infect Control Hosp Epidemiol. 1994; 15:520-8.

  • 3. Go ES Urban C Burns J Kreiswirth B Eisner W Mariano N. Clinical and molecular epidemiology of Acinetobacter infections sensitive only to polymyxin B and sulbactam. Lancet. 1994; 12:1329-32.

  • 4. Roberts SA Findlay R Lang SDR. Investigation of an outbreak of multi-drug resistant Acinetobacter baumannii in an intensive care burns unit. J Hosp Infect. 2001; 48:228-32.

  • 5. Yu YS Yang Q Xu XW Kong HS Xu GY Zhong BY. Typing and characterization of carbapenem-resistant Acinetobacter calcoaceticus-baumannii complex in a Chinese hospital. J Med Microbiol. 2004; 53:653-6.

  • 6. Zarrilli R Crispino M Bagattini M. Molecular epidemilogy of sequential outbreaks of Acinetobacter baumannii in an intensive care unit shows the emergence of carbapenem resistance. J Clin Microbiol. 2004; 42:946-53.

  • 7. Wareham DW Bean DC Khanna P Hennessy EM Krahe D Ely A Millar M. Bloodstream infection due to Acinetobacter spp: epidemiology risk factors and impact of multi-drug resistance. Eur J Clin Microbiol Infect Dis. 2008; 27:607-12.

  • 8. Lortholary O Fagon JY Hoi AB Slama MA Pierre J Giral P. Nosocomial acquisition of multiresistant Acinetobacter baumannii: risk factors and prognosis. Clin Infect Dis. 1005; 20:790-6.

  • 9. Barbolla RE Centron D Maimone S Rospide F Salgueira C Altclas J et al. Molecular epidemiology of Acinetobacter baumannii spread in an adult intensive care unit under an endemic setting. Am J Infect Control. 2008; 36:444-52.

  • 10. Scerpella EG Wanger AR Armitige L Anderlini P. Nosocomial outbreak caused by a multiresistant clone of Acinetobacter baumannii: results of the casecontrol and molecular epidemiologic investigations. Infect Control Hosp. 1995; 16:92-7.

  • 11. Houang ETS Chu YW Leung CM. Epidemiology and infection control implications of Acinetobcter spp. In Hong Kong. J Clin Microbiol. 2001; 39:228-34.

  • 12. Maragakis LL Cosgrove SE Song X. An outbreak of multi-drug resistant Acinetobacter baumannii associated with pulsatile lavage wound treatment. JAMA. 2004; 292:3006-11.

  • 13. Chu YW Afzal-Shah M Houang ET Palepou ML Lyon DJ Woodford N. IMP-4 a novel metallo-b-lactamase from nosocomial Acinetobacter spp. Antimicrob Agents Chemother. 2001; 45:710-14.

  • 14. Welsh J McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nuc Acids Res. 1990; 18:7213-18.

  • 15. Seifert H Schulze A Baginski R Pulverer G. Comparison of four different methods for epidemiologic typing of Acinetobacter baumannii. J Clin Microbiol. 1994; 32:1816-19.

  • 16. Koeleman JGM Stoof J Vandenbroucke-Gruls CM Savelkoul PHM. Identification of epidemic strains of Acinetobacter baumannii by integrase gene PCR. J Clin Microbiol. 2001; 39:8-13.

  • 17. Prashanth K Badrinath S. Epidemiological investigation of nosocomial Acinetobacter infections using arbitrarily primed PCR & pulse field gel electrophoresis. Indian J Med Res. 2005; 122:408-18.

  • 18. Das I Lamberty P Hill D Noy M Bion J Elliott T. Carbapenem-resistant Acinetobacter and role of curtains in an outbreak in intensive care units. J Hosp Infect. 2002; 50:110-14.

  • 19. Killgore GE Kato H. Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing. J Clin Microbiol. 1994; 32:1591-3.

  • 20. Le Hello S Falcot V Lacassin F Baumann F Nordmann P Naas T. Molecular epidemiology of carbapenemresistant Acinetobacter baumannii in New Caledonia. Clin Microbiol Infect. 2008; 14:977-81.

  • 21. Lin MF Yang CM Lin CH Huang ML Tu CC Liou ML. Clinical features and molecular epidemiology of multidrug-resistant Acinetobacter calcoaceticus-A baumannii complex in a regional teaching hospital in Taiwan. Am J Infect Control. 2009 37:e1-3.

  • 22. Wendt C Dietze B Dietz E Ruden H. Survival of Acinetobacter baumannii on dry surfaces. J Clin Microbiol. 1997; 35:1394-7.

  • 23. Smolyakov R Borer A Riesenberg K. Nosocomial multi-drug resistant Acinetobacter baumannii bloodstream infection: risk factors and outcome with ampicillin-sulbactam treatment. J Hosp Infect. 2003; 54:32-8.

  • 24. Wang SH Shengy WH Chang YY. Healthcareassociated outbreak due to pan-drug resistant Acinetobacter baumannii in a surgical intensive care units. J Hosp Infect. 2003; 53:97-102.

  • 25. Basustaoglu AC Kisa O Sacilik SC Ozyurt M. Epidemiological characterization of hospital-acquired Acinetobacter baumannii isolates from a 1500-bed teaching hospital by phenotypic and genotypic methods. J Hosp Infect. 2001; 47:246-9.

  • 26. Ayan M Durmaz R Aktas E Durmaz B. Bacteriological clinical and epidemiological characteristics of hospital-acquired Acinetobacter baumannii infection in a teaching hospital. J Hosp Infect. 2003; 54:39-45.

Search
Journal information
Impact Factor
IMPACT FACTOR 2018: 0.2
5-year IMPACT FACTOR: 0.293

CiteScore 2018: 0.30

SCImago Journal Rank (SJR) 2018: 0.172
Source Normalized Impact per Paper (SNIP) 2018: 0.237

Cited By
Metrics
All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 215 92 3
PDF Downloads 156 87 1