Relationship between vitamin D receptor gene polymorphisms and anemia in postmenopausal Vietnamese women

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Background: Both in vitro and in vivo studies have shown that calcitriol, the active form of vitamin D, is involved in hematopoiesis. Vitamin D receptor (VDR) gene has been suggested as one of the candidate genes for anemia. Objective: Investigate relationship between anemia and the commonly studied polymorphisms of VDR gene (FokI, BsmI, ApaI and TaqI) in terms of genotype and haplotype in Vietnamese. Methods: A case-control study including 132 postmenopausal women without chronic kidney diseases was designed to investigate the relationship between VDR polymorphism and anemia. Four single nucleotide polymorphisms (SNPs) FokI (rs2228570), BsmI (rs1544410), ApaI (rs7975232), and TaqI (rs731236) were typed by polymerase chain reaction and restriction fragment length polymorphism method. Results: Genotype distributions of four SNPs were in Hardy-Weinberg equilibrium in both anemia and control groups. The SNPs at the 3’end of the VDR gene (BsmI, ApaI and TaqI) exhibited a strong linkage disequilibrium. There was no significant association between anemia and VDR polymorphism in terms of allele, genotype, and haplotype in the analyses unadjusted or adjusted for the covariates (age, body mass index, educational level, serum ferritin, iron and albumin). Conclusion: VDR gene did not influence anemia in postmenopausal women without chronic kidney disease. For further study on the association between VDR gene and anemia, the use of larger sample size, a prospective study design, and additional markers would enhance the reliability and validity of findings.

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  • 1. Heaney RP. Vitamin D in Health and Disease. Clin J Am Soc Nephrol. 2008; 3:1535-41.

  • 2. Bunce CM Brown G Hewison M. Vitamin D and hematopoiesis. Trends Endocrinol Metab. 1997; 8: 245-51.

  • 3. Sim JJ Lac PT Liu IL Meguerditchian SO Kumar VA Kujubu DA Rasgon SA. Vitamin D deficiency and anemia: a cross-sectional study. Ann Hematol. 2010; 89:447-52.

  • 4. Swamy N Xu WR Paz N Hsieh JC Haussler MR Maalouf GJ et al. Molecular modeling affinity labeling and site-directed mutagenesis define the key points of interaction between the ligand-binding domain of the vitamin D nuclear receptor and 1R25-dihydroxyvitamin. Biochemistry. 2000; 39:12162-71.

  • 5. Bhanushali AA Lajpal N Kulkarni SS Chavan SS Bagadi SS Das BR. Frequency of fokI and taqI polymorphism of vitamin D receptor gene in Indian population and its association with 25-hydroxyvitamin D levels. Indian J Hum Genet. 2009; 15:108-13.

  • 6. Uitterlinden AG Fang Y van Meurs JB Pols HA Van Leeuwen JP. Genetics and biology of vitamin D receptor polymorphisms. Gene. 2004; 338:143-56.

  • 7. Fang Y van Meurs JB d’Alesio A Jhamai M Zhao H Rivadeneira F et al. Promoter and 3’-untranslatedregion haplotypes in the vitamin D receptor gene predispose to osteoporotic fracture: the Rotterdam study. Am J Hum Genet. 2005; 77:807-23.

  • 8. Crofts LA Hancock MS Morrison NA Eisman JA. Multiple promoters direct the tissue-specific expression of novel N-terminal variant human vitamin D receptor gene transcripts. Proc Natl Acad Sci USA. 1998; 95:10529-34.

  • 9. Zmuda JM Cauley JA Ferrell RE. Molecular epidemiology of vitamin D receptor gene variants. Epidemiol Rev. 2000; 22:203-17.

  • 10. WHO/UNICEF/UNU. Iron Deficiency Anaemia: Assessment Prevention and Control. A Guide for Programme Managers. Geneva:World Health Organization. 2001. WHO/NHD/01.3.

  • 11. Mitra S Desai M Ikram Khatkhatay M. Vitamin D receptor gene polymorphisms and bone mineral density in postmenopausal Indian women. Maturitas. 2006; 55:27-35.

  • 12. Sole X Guino E Valls J Iniesta R Moreno V. SNPStats: a web tool for the analysis of association studies. Bioinformatics. 2006; 22:1928-29.

  • 13. Ertürk S Kutlay S Karabulut HG Keven K Nergizoglu G Ates K et al. The impact of vitamin D receptor genotype on the management of anemia in hemodialysis patients. Am J Kidney Dis. 2002; 40: 816-23.

  • 14. Sezer S Tutal E Bilgic A Ozdemir FN Haberal M. Possible influence of vitamin D receptor gene polymorphisms on recombinant human erythropoietin requirements in dialysis patients. Transplant Proc. 2007; 39:40-4.

  • 15. Thakkinstian A D’Este C Attia J. Haplotype analysis of VDR gene polymorphisms: a meta-analysis. Osteoporos Int. 2004; 15:729-34.

  • 16. Nejentsev S Godfrey L Snook H Rance H Nutland S Walker NM et al. Comparative high-resolution analysis of linkage disequilibrium and tag single nucleotide polymorphisms between populations in the vitamin D receptor gene. Hum Mol Genet. 2004; 13:1633-9.

  • 17. Raimondi S Johansson H Maisonneuve P Gandini S. Review and meta-analysis on vitamin D receptor polymorphisms and cancer risk. Carcinogenesis. 2009; 30:1170-80.

  • 18. Zhou H Xu C Gu M. Vitamin D receptor (VDR) gene polymorphisms and Graves’ disease: a metaanalysis. Clin Endocrinol. 2009; 70:938-45.

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