Effects of intense exercise on biochemical and histological changes in rat liver and pancreas

Abstract

Background: Exercise has various effects on hepatic and pancreatic function. There is little information available for effects of exercise on histopathological changes in the liver and pancreas. Objective: Investigate the effect of intensive exercise on blood biochemical changes and histopathology in rat liver and pancreas. Materials and methods: Male Sprague-Dawley rats were randomly divided into five groups: 1 (normal control): no exercise, 2 (exercise 75% VO2max): running on treadmill at 75% VO2max and sacrificed immediately after exercise, 3 (exercise 75% VO2max+6 hours): running on treadmill at 75% VO2max and sacrificed at six hours after exercise, 4 (exercise 90% VO2max): running on treadmill at 90% VO2max and immediately sacrificed after exercise, 5 (exercise 90% VO2max+6 hours): running on treadmill at 90% VO2max and sacrificed at six hours after exercise. Samples of blood, liver and pancreas were collected at the end of each experiment. Results: The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) increased significantly in group 2 and 4, compared with normal control. Serum total bilirubin (TB) and enzyme lipase increased significantly in group 4 compared with normal control. In all group of exercise, liver histopathology showed hepatocyte edema and necroinflammation, and pancreas showed congestion and edema. Conclusion: High-intensity exercise at 75% and 90% VO2max caused an increase of biochemical parametes in liver and pancreas. The levels of exercise also caused histopathology changes in the liver and pancreas.

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  • 1. Gorski J, Oscai LB, Palmer WK. Hepatic lipid metabolism in exercise and training. Med Sci Sports Exercise. 1990; 22:213-21.

  • 2. Simonelli C, Eaton RP. Reduced triglyceride secretion: a metabolic consequence of chronic exercise. Am J Physiol. 1978; 234:E221-7.

  • 3. Reddy Avula CP, Fernandes G. Modulation of antioxidant enzymes and lipid peroxidation in salivary gland and other tissues in mice by moderate treadmill exercise. Aging (Milano). 1999; 11:246-52.

  • 4. Venditti P, Di Meo S. Antioxidants, tissue damage, and endurance in trained and untrained young male rats. Arch Biochem Biophys. 1996; 331:63-8.

  • 5. Dyke TM, Sams RA, Hinchcliff KW. Exercise-traininginduced alterations in hepatic function in mares. J Appl Physiol. 1998; 85:1442-7.

  • 6. Mauriz JL, Tabernero B, Garcia-Lopez J, Jorquera F, Villa JG, Gonzalez-Gallego J. Physical exercise and Improvement of liver oxidative metabolism in the elderly. Eur J Appl Physiol. 2000; 81:62-6.

  • 7. Minato K. The effect of chronic exercise on the rat Pancreas. Int J Pancreatol. 2000; 27:151-6.

  • 8. Konturek SJ, Tasker J, Obtulowicz W. Effect of exercise on gastrointestinal secretion. J Appl Physiol. 1973; 34, 324-8.

  • 9. Minato K. The Effect of endurance training on pancreatic enzyme activity in rats. Eur J Appl Physiol Occup Physiol. 1997; 76,491-4.

  • 10. Bedford TG, Tipton CM, Wilson NC, Oppliger RA, Gisolfi CV. Maximum oxygen consumption of rats and its changes with various experimental procedures. J Appl Physiol. 1979; 47:1278-83.

  • 11. Brunt EM, Janney CG, Di Bisceglie AM, Neuschwander-Tetri BA, Bacon BR. Nonalcoholic steatohepatitis: a proposal for grading and staging the histological lessions. Am J Gastroenterol. 1999; 94: 2467-74.

  • 12. Rowell LB, Blackmon JR, Bruce RA. Indocyanine green clearance and estimated hepatic blood flow during mild to maximal exercise in upright man. J Clin Invest. 1964; 43:1677-90.

  • 13. Ohnishi K, Saito M, Nakayama T, Iida S, Nomura F, Koen H, Okuda K. Portal venous hemodynamics in chronic liver disease: effects of posture change and exercise. Radiology. 1985; 155:757-61.

  • 14. Yano H, Kinoshita S, Yano L. Acute exercise induces mitocondria swelling of hepatocytes surrounding the terminal hepatic venule in rat liver acinus. Jpn J Phys Fitness Sports Med. 1997; 46:49-54.

  • 15. Latour MG, Brault A, Huet PM, Lavoie JM. Effects of acute physical exercise on hepatocyte volume and fuction in rat. Am J Physiol. 1999; R1258-64.

  • 16. Yano L, Yano H, Takeda K. Electromagnetic determination of portal venous flow in rats during exercise. Inter Hepato Commun. 1996; 5:184-90.

  • 17. Fojt E, Ekelund L-G, Hultman E. Enzyme activities in hepatic venous blood under strenous physical exercise. Pflugers Arch. 1976; 361:287-96.

  • 18. McCord JM. Oxygen-derived radicals: a link between reperfusion injury and inflammation. Fed Proc. 1987; 46:2402-6.

  • 19. Caraceni P, Rosenblum ER, Van Thiel DH, Borle AB. Reoxygenation injury in isolated rat hepatocytes: relation to oxygen free radicals and lipid peroxidation. Am J Physiol. 1994; 266:G799-806.

  • 20. Zsinka AJ, Frenkl R. Exocrine function of the pancreas in regularly swimming rats. Acta Physiol Hung. 1983; 62:123-9.

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