Association between angiotensin II type-1 receptor A1166C polymorphism and the presence of angiographically-defined coronary artery disease in an Iranian population

Background: There are reported associations between a polymorphism of the angiotensin II type 1 receptor (AT₁R/A1166C) gene and coronary artery disease (CAD), hypertension, and myocardial infarction in some populations. Objective: Investigate the association between A1166C polymorphism and CAD in an Iranian population. Methods: Four hundred and thirteen patients with suspected CAD were recruited. Based on coronary angiography, the patients were classified into CAD+ (n=315) and CAD- (n=98) groups defined as >50% and <50% stenosis of any major coronary artery, respectively. One hundred and thirty-five healthy subjects were also recruited as the control group. The AT₁R polymorphism was assessed using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) based method. Results: A higher frequency of the AC and CC genotypes and lower frequency of the AA genotype was observed in both CAD+ and CAD- groups, compared with the control group (p <0.05). CAD+ and CAD- groups also had a higher frequency of the C allele than controls (p <0.01). There was no significant difference in genotype and allele frequencies between hypertensive and non-hypertensive patients (p > 0.05). In addition, the AT₁R genotype frequencies did not differ significantly among different subgroups of CAD+ patients, based on the number of affected coronary vessels (p >0.05). Conclusion: The frequency AT₁R/A1166C polymorphism was higher among patients with some degrees of coronary stenosis who are candidates of coronary angiography.