Dpph Radical Scavenging Activity and Phenolic Compound Content in Different Leaf Extracts from Selected Blackberry Species

Open access

Abstract

We used DPPH scavenging assays to study the antioxidant activity of three native Polish species of blackberry leaves (Rubus kuleszae Ziel., R. fabrimontanus (Sprib.) Sprib. and R. capitulatus Utsch.). All the studied extracts (methanolic, water, methanolic-water) showed high DPPH free radical scavenging activity (IC50 450.0-186.0 μg/ml). The most effective of the studied species was Rubus kuleszae. Total content of phenolic compounds (70.50-136.04 mg GAE/g) and phenolic acids (14.70-38.26 mg CAE/g) was determined spectrophotometrically. Antioxidant activity correlated positively with total content of phenolic compounds and phenolic acids.

References
  • Ali L, Alsanius BW, Rosberg AK, Svensson B, Nielsen T, and Olsson ME. 2012. Effects of nutrition strategy on the levels of nutrients and bioactive compounds in blackberries. European Food Research and Technology 234: 33-44.

  • Alice LA, and Campbell CS. 1999. Phylogeny of Rubus (Rosaceae) based on nuclear ribosomal DNA internal transcribed spacer region sequences. American Journal of Botany 86: 81-97.

  • Assimopoulou AN, Sinakos Z, and Papageorgiou VP. 2005. Radical scavenging activity of Crocus sativus L. extract and its bioactive constituents. Phytotherapy Research 19: 997-1000.

  • Benvenuti S, Pellati F, Melegari M, and Bertelli D. 2004. Polyphenols, anthocyanins, ascorbic acid, and radical scavenging activity of Rubus, Ribes, and Aronia. Journal of Food Science 69: 164-169.

  • Brand-Williams W, Cuvelier ME, and Berset C. 1995. Use of a free radical method to evaluate antioxidant activity, Lebensmittel-Wissenschaft und-Technologie 28: 25-30.

  • Byamukama R, Kiremire BT, Andersen ØM, and Steigen A. 2005. Anthocyanins from fruits of Rubus pinnatus and Rubus rigidus. Journal of Food Composition and Analysis 18: 599-605.

  • Dall'Acqua S, Cervellati R, Loi MC, and Innocenti G. 2008. Evaluation of in vitro antioxidant properties of some traditional Sardinian medicinal plants: Investigation of the high antioxidant capacity of Rubus ulmifolius. Food Chemistry 106: 745-749.

  • Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P, and Vidal N. 2006. Antioxidant activity of some algerian medicinal plants extracts containing phenolic compound. Food Chemistry 97: 654-660.

  • Gudej J, and Tomczyk M. 2004. Determination of flavonoids, tannins and ellagic acid in leaves from Rubus L. species. Archives of Pharmacal Research 27: 1114-1119

  • JABłOŃSKA-RYŚ E, Zalewska-Korona M, and Kalbarczyk J. 2009. Antioxidant capacity, ascorbic acid and phenolics content in wild edible fruits. Journal of Fruit and Ornamental Plant Research 17: 115-120.

  • Jiao Z, Liu J, and Wang S. 2005. Antioxidant activities of total pigment extract from Blackberries, Food Technology and Biotechnology 43: 97-102.

  • Kluza-Wieloch M and Maciejewska-Rutkowska I. 2009. Rhythmic development of some native species of blackberries (Rubus L., Corylifolii, Rosaceae) from the collection of Dendrological Garden of University of Life Sciences in Poznań, Herba Polonica 55: 38-46.

  • Komes D, BelšÈak-Cvitanović A, HORŽIĆ D, Rusak G, Likićb S, and Berendika M. 2011. Phenolic composition and antioxidant properties of some traditionally used medicinal plants affected by the extraction time and hydrolysis. Phytochemical Analysis 22: 172-180.

  • Li H, Wong C, Cheng K, and Chen F. 2008. Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT - Food Science and Technology 41: 385-390.

  • Li X, Wu X, and Huang L. 2009. Correlation between Antioxidant Activities and Phenolic Contents of Radix Angelicae Sinensis (Danggui). Molecules 14: 5349-5361.

  • Mertz C, Cheynier V, Günata Z, and Brat P. 2007. Analysis of phenolic compounds in two blackberry species (Rubus glaucus and Rubus adenotrichus) by high-performance liquid chromatography with diode array detection and electrospray ion trap mass spectrometry. Journal of Agricultural and Food Chemistry 55: 8616-8624.

  • Molyneux P. 2004. The use of the free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology 25: 211-219.

  • Patel AV, Rojas-Vera J, and Dacke CG. 2004. Therapeutic constituents and actions of Rubus species. Current Medicinal Chemistry 11: 1501-1512.

  • Pietta PG. 2000. Flavonoids as antioxidants. Journal of Natural Product 63: 1035-1042.

  • Polish Pharmacopeia VI. 2002. Polskie Towarzystwo Farmaceutyczne, Warszawa.

  • Reyes-Carmona J, Yousef GG, Martínez-Peniche RA, and Lila MA. 2005. Antioxidant capacity of fruit extracts of Blackberry (Rubus sp.) produced in different climatic regions. Journal of Food Science 70: 497-503.

  • Rice-Evans CA, Miller NJ, and Paganga G. 1996. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine 20: 933-956.

  • Thiem B. 2003. Rubus chamaemorus L. - a boreal plant rich in biologically active metabolites: a review. Biology Letters 40: 3-13.

  • Venskutonis PR, Dvaranauskaite A, and Labokas J. 2007. Radical scavenging activity and composition of raspberry (Rubus idaeus) leaves from different locations in Lithuania. Fitoterapia 78: 162-165.

  • Villańo D, Fernández-Pachón MS, Moyá ML, Troncoso AM, and García-Parrilla MC. 2007. Radical scavenging ability of polyphenolic compounds towards DPPH free radical. Talanta 71: 230-235.

  • Wang SY, and Lin HS. 2000. Antioxidant activity in fruits and leaves of blackberry, raspberry, and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry 48: 140-146.

  • Witkowska A, and Zujko ME. 2009. Aktywność antyoksyda-cyjna owoców leśnych. Bromatologia i Chemia Toksykologiczna XLII: 900-903.

  • Zieliński J. 2004. The genus Rubus (Rosaceae) in Poland. Polish Botanical Studies 16: 1-300.

Acta Biologica Cracoviensia s. Botanica

The Journal of Polish Academy of Sciences

Journal Information


IMPACT FACTOR 2016: 0.491
5-year IMPACT FACTOR: 0.787

CiteScore 2016: 0.51

SCImago Journal Rank (SJR) 2016: 0.242
Source Normalized Impact per Paper (SNIP) 2016: 0.264

Ministry of Science and Higher Education: 20 points

Metrics

All Time Past Year Past 30 Days
Abstract Views 0 0 0
Full Text Views 82 82 62
PDF Downloads 23 23 13