Drought Stress Effects on Photosynthesis, Chlorophyll Fluorescence and Water Relations in Tolerant and Susceptible Chickpea (Cicer Arietinum L.) Genotypes
In order to evaluate morphological and physiological traits related to drought tolerance and to determine the best criteria for screening and identification of drought-tolerant genotypes, we grew two tolerant genotypes (MCC392, MCC877) and two sensitive genotypes (MCC68, MCC448) of chickpea under drought stress (25% field capacity) and control (100% field capacity) conditions and assessed the effect of drought stress on growth, water relations, photosynthesis, chlorophyll fluorescence and chlorophyll content in the seedling, early flowering and podding stages. Drought stress significantly decreased shoot dry weight, CO2 assimilation rate (A), transpiration rate (E), and Psii photochemical efficiency (Fv/Fm) in all genotypes. In the seedling and podding stages, Psii photochemical efficiency was higher in tolerant genotypes than in sensitive genotypes under drought stress. Water use efficiency (WUE) and CO2 assimilation rate were also higher in tolerant than in sensitive genotypes in all investigated stages under drought stress. Our results indicated that water use efficiency, A and Fv/Fm can be useful markers in studies of tolerance to drought stress and in screening adapted cultivars of chickpea under drought stress.
Ahmed S, Nawata E, Hosokawa M, Domae Y, and Sakuratani T. 2002. Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Science 163: 117-123.
Arnon D. 1949. Copper enzymes in isolated chloroplasts: polyphenol oxidases in Beta vulgaris. Plant Physiology 24: 1-15.
Ashraf M, Nawazish SH, and Athar H. 2007. Are chlorophyll fluorescence and photosynthetic capacity potential physiological determinants of drought tolerance in maize (Zea mays L.). Pakistan Journal of Botany 39: 1123-1131.
Barr HD, and Weatherley PE. 1962. A re-examination of the relative turgidity technique for estimating water deficit in leaves. Australian Journal of Biological Science 15: 413-428.
Bayoumi TY, Eid M, and Metwali EM. 2008. Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. African Journal of Biotechnology 7: 2341-2352.
Dulai S, Molnar I, Pronay J, Csernak A, Tarnai R, and Molnarlang M. 2006. Effects of drought on photosynthetic parameters and heat stability of PSII in wheat and in Aegilops species originating from dry habitats. Acta Biologica Szegediensis 50: 11-17.
Farooq M, Wahid A, Kobayashi N, Fujita D, and Basra SMA. 2009. Plant drought stress: effects, mechanisms and management. Agronomy for Sustainable Development 29: 153-188.
Galle A, Csiszar J, Tari I, and Erdei L. 2002. Changes in water and chlorophyll fluorescence parameters under osmotic stress in wheat cultivars. Acta Biologica Szegedieniensis 46(3-4): 85-86
Gindaba J, Rozanov A, and Negash L. 2004. Photosynthetic gas exchange, growth and biomass allocation of two Eucalyptus and three indigenous tree species of Ethiopia under moisture deficit. Forest Ecology and Management 205:127-138
Gunes A, Cicek N, Inal A, Alpaslan M, Eraslan F, Guneri E, and Guzelordu T. 2006. Genotypic response of chickpea (Cicer arietinum L.) cultivars to drought stress implemented at pre- and postanthesis stages and its relations with nutrient uptake and efficiency. Plant Soil Environment 52: 368-376.
Hussein MM, Kassab OM, and Ellil AA. 2008. Evaluating water stress influence on growth and photosynthetic pigments of two sugar beet varieties. Research Journal of Agriculture and Biological Sciences 4(6): 936-941.
Jaleel CA, Gopi R, and Panneerselvam R. 2008a. Growth and photosynthetic pigments responses of two varieties of Catharanthus roseus to triadimefon treatment. Comptes Rendus Biologies 331: 272-277.
Jaleel CA, Manivannan P, Lakshmanan GMA, Gomathinayagam M, and Panneerselvam R. 2008b. Alterations in morphological parameters and photosynthetic pigment responses of Catharanthus roseus under soil water deficits. Colloids and Surfaces B: Biointerfaces 61: 298-303.
Jaleel CA, Manivannan P, Wahid A, Farooq M, AL-Juburi HJ, Somasundaram R, and Panneerselvam R. 2009. Drought stress plants: a review on morphological characteristics and pigments composition. International Journal of Agriculture and Biology 11: 100-105.
Jinmin FU, and Huang B. 2001. Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environmental and Experimental Botany 45: 5-11.
Kauser R, Athar HUR, and Ashraf M. 2006. Chlorophyll fluorescence: a potential indicator for assessment of water stress tolerance in canola (Brassica napus L.). Pakistan Journal of Botany 38(5): 1501-1509.
Kiani SP, Maury P, Sarrafi A, and Grieu P. 2008. QTL analysis of chlorophyll fluorescence parameters in sunflower (Helianthus annuus L.) under well-watered and waterstressed conditions. Plant Science 175: 565-573.
Labidi N, Mahmoudi H, Dorsaf M, Slama I, and Abdelly C. 2009. Assessment of intervarietal differences in drought tolerance in chickpea using both nodule and plant traits as indicators. Journal of Plant Breeding and Crop Science 1: 80-86.
Lu C, and Zhang J. 1998. Effects of water stress on photosynthesis, chlorophyll fluorescence and photoinhibition in wheat plants. Australian Journal of Plant Physiology 25: 883-892
Maxwell K, and Johnson GN. 2000. Chlorophyll fluorescence-a practical guide. Journal of Experimental Botany 51(345): 659-668.
Mssacci A, Nabiev SM, Pietrosanti L, Nematov SK, Chernikova TN, Thor K, and Leipner J. 2008. Response of photosynthetic apparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging. Plant Physiology and Biochemistry 46: 189-195.
Nageswara Rao RC, Williams JH, Wadia KDR, Hubick KT, and Farquhar GD. 2008. Crop growth, water-use efficiency and carbon isotope discrimination in groundnut (Arachis hypogaea L.) genotypes under end-of season drought conditions. Annals of Applied Biology 122: 357-367.
Nam NH, Chauhan YS, and Johansen C. 2001. Effect of timing of drought stress on growth and grain yield of extrashort-duration pigeon pea lines. Journal of Agriculture Science 136: 179-189.
Parameshwarappa SG, and Salimath PM. 2008. Field screening of chickpea genotypes for drought resistance. Karnataka Journal of Agriculture Science 21: 113-114.
Piper FI, Corcuera LJ, Alberdi M, and Lusk C. 2007. Differential photosynthetic and survival responses to soil drought in two evergreen Nothofagus species. Annals of forest Sciences 64: 447-452.
Premachandra GS, Saneoka H, and Ogata S. 1990. Cell membrane stability an indicator of drought tolerance as affected by applied nitrogen in soybean. Journal of Agricultural Science 115: 63-66.
Sairam RK 1994. Effect of moisture stress on physiological activities of two contrasting wheat genotypes. Indian Journal of Experimental Biology 32: 593-594.
Sairam RK, Deshmukh PS, and Shukla DS. 1997. Tolerance of drought and temperature stress in relation to increased antioxidant enzyme activity in wheat. Journal of Agronomy and Crop Science 178: 171-178.
Sayed OH. 2003. Chlorophyll fluorescence as a tool in cereal crop research. Photosynthetica 41(3): 321-330.
Siddique MRB, Hamid A, and Islam MS. 2000. Drought stress effects on water relations of wheat. Botanical Bulletin of Academia Sinica 41: 35-39.
Simova-Stoilova L, Demirevska K, Petrova T, Tsenov N, and Feller U. 2008. Antioxidative protection in wheat varieties under severe recoverable drought at seedling stage. Plant Soil Environment 54: 529-536.
Tahkokorpi M, Taulavuori K, Laine K, and Taulavuori E. 2007. After-effects of drought related winter stress in previous and current year stems of Vaccinium myrtillus L. Environmental and Experimental Botany 61: 85-93.
Terzi R, and Kadioglu A. 2006. Drought stress tolerance and antioxidant enzyme system in Ctenanthe setosa. Acta Biologica Cracoviensia Series Botanica 48: 89-96.
Toker C, and Çagirgan M. 1998. Assessment of response to drought stress of chickpea (Cicer arietinum L.) lines under rain field conditions. Turkish Journal of Agriculture and Forestry 22: 615-621.
Zlatve ZS, and Yordanov IT. 2004. Effects of soil drought on photosynthesis and chlorophyll fluorescence in bean plants. Bulgarian Journal of Plant Physiology 30: 3-18.