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Anna Lis, Rozalia Liszkiewicz and Agnieszka Krajewska

. Brijesh K, Ruchi R, Sanjita D, Saumaya D. Phytoconstituents and therapeutic potential of Thuja occidentalis . Res J Pharm Biol Chem Sci 2012; 3(2):354-362. 6. Jasuja ND, Sharma D, Choudhary J, Joshi SC. Essential oil and important activities of Thuja orientalis and Thuja occidentalis . J Essent Oil Bearing Pl 2015; 18(4):931-949. http://dx.doi.org/10.1080/0972060X.2014.884774 7. Akkol EK, Ilhan M, Demirel MA, Keles H, Tumen I, Suntar I. Thuja occidentalis L. and its active compound, α -thujone: promising effect in the treatment of polycystic ovary

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A. Marcin, M. Levkut, V. Revajová, B. Šoltysová and P. Naď

., Rozkot, M., 2005: Influence of lecithin emulsifier on the utilisation of nutrients and growth of piglets after weaning. Czech. J. Anim. Sci., 50, 459-465. 9. Durling, N. E., Catchpole, O. J., Grey, J. B., Webby, R. F., Mitchell, K. A., Yeap Foo, L., Perry, N. B., 2007: Extraction of phenolics and essential oil from dried sage (Salvia officinalis) using ethanol-water mixtures. Food Chemistry, 101, 1417-1424. 10. Emmert, J., Sartor, G., Sporer, F., Gummersbach, J., 2004: Determination of α-/β-thujone and related terpemens in absinthe using

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Grażyna Zawiślak

Summary

The study upon sage (Salvia officinalis L.) has been carried out at the Experimental Section of Department of Vegetables and Medicinal Plants, University of Life Sciences in Lublin. The aim of present study was to evaluate the yield and chemical composition of essential oil extracted from sage leaves harvested from a three-year plantation. The sage herb harvest date in the third year of cultivation had a significant impact on the yield of plants. Higher yield of fresh and dry herb, dry leaves, and essential oil was achieved in August (the second harvest time) than in May (the first harvest time). Chromatographic examination indicated the presence of 50 chemical compounds in sage essential oil. It was a variable percentage of essential oil components depending on the raw material harvest time. The main components of the sage essential oil were: 1,8-cineole (16.08-18.04%), α-thujone (10.40-21.51%) and camphor (5.24-18.08%).

Open access

Miloš Nikolić, Tatjana Marković, Dejan Marković, Jasmina Glamočlija, Ana Ćirić, Marija Smiljković and Marina Soković

Summary

Chemical composition, antimicrobial and cytotoxic activities of commercial essential oils’ samples from the aerial plant parts of H. officinalis, R. officinalis and S. officinalis were investigated. Analyses by GC-FID and GC-MS confirmed 52 oil components. The major constituent of the H. officinalis oil was cis-pinocamphone (34.4%), followed by transpinocamphone (23.3%), and β-pinene (11.3%). Analysis of R. officinalis oil revealed 1.8-cineol as a major constituent (43.8%), as well as transpinocamphone (12.5%), α-pinene (11.5%) and β-pinene (8.2%). The most dominant constituent of S. officinalis oil was cis-thujone (32.7%), in addition to camphor (17.2%), 1.8-cineol (10.1%), α-pinene (8.6%), transthujone (7.7%) and camphene (7.3%). The essential oil antimicrobial activity assay was performed by the use of microdilution method against oral Candida spp. and bacteria, the major causative agents of a number of human oral disorders; all of them were susceptible to tested concentrations of H. officinalis, R. officinalis and S. officinalis essential oils, although the oil of S. officinalis exhibited the lowest antimicrobial potential. The results obtained in this study encourage use of investigated essential oils from Lamiaceae family in development of safe natural agents for prevention and/ or alternative therapy of human oral diseases. However, a special care during development of an effective natural preparation is required.

Open access

Soňa Jantová, Roman Hudec, Stanislav Sekretár, Juraj Kučerák and Martina Melušová

, Lachenmeier DW. (2011). Determination of the biologically active flavour substances thujone and camphor in foods and medicines containing sage (Salvia offi cinalis L.). Chem Central J 5: 1-10. Willcox JK, Ash SL, Catignani GL. (2004). Antioxidants and prevention of chronic disease. Crit Rev Food Sci Nutr 44: 275-295. Wu ZB, Ni ZY, Sh, QW, Dong M, Kizota H, Gu ZCh, Cong B. (2012). Constituents from Salvia species and their biological activities. Chem Rev 112: 5967-6026.

Open access

Nuriye Nuray Ulusu

of curare. J Ethnopharmacol 1992; 36: 1-26. 40. Bliss CL, Novy FG. Action of formaldehyde on enzymes and on certain pboteids. J Exp Med 1899; 1: 47-80. 41. Cao L. Immobilised enzymes: science or art? Current Opinion in Chemical Biology 2005; 9: 217-26. 42. Pelkonen O, Abass K, Wiesner J. Thujone and thujonecontaining herbal medicinal and botanical products: Toxicological assessment. Regul Toxicol Pharmacol 2013; 65: 100-7. 43. Abass K, Reponen P, Mattila S, Pelkonen O. Metabolism of a-thujone in human