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Adrian Maier, Adrian Man, Călin Chibelean, Teodora Cighir, Eniko Nemes-Nagy, Ioana Maier, Ciprian Todea, Oliver Vida and Orsolya Martha

Abstract

Objective: To evaluate the bacteriological features in non-struvite nephrolithiasis and in its associated urinary tract infection, and to establish the relationship between the two pathologies.

Methods: The non-struvite calculi from 132 patients were aseptically extracted by percutaneous nephrolithotomy (PNL). The midstream urine and calculi were bacteriologically and biochemically processed.

Results: Most calculi (78%) were located to renal pelvis, associated with hydronephrosis, the biochemical composition confirming the lack of struvite and revealing the predominance of calcium oxalate. The females presented significantly more colonized calculi (50%) than males (21.9%), with higher bacteriological diversity. There is a significant relation between the presence of colonized calculi and urinary tract infections, 24.2% of calculi and 25.8% of the urine samples presenting positive cultures. In 70.4% of cases, we found the same antibiotic resistance pattern between the pathogens isolated from calculi and urine, thus considering them identical strains. The Enterobacteriaceae represented the most predominant bacteria both from calculi (62.5%) and urine (63.6%), approximatively 30% being resistant to cephalosporins and over 50% resistant to fluoroquinolones, ampicillin and tetracycline. There were 3.8% of cases in which the calculi were colonized but the urine was sterile, the bacteria being sensitive to cephalosporins that are used as prophylaxis.

Conclusions: In all the cases, the same bacterial species was found both in calculi and urine, and 70.4% of them were phenotypically identical. The resistance to the second generation cephalosporins is lower than in the case of other antibiotics, which makes them the most suitable for prophylaxis in PNL.

Open access

T. Grenda, E. Kukier, Z. Sieradzki, M. Goldsztejn and K. Kwiatek

Abstract

This study was undertaken to examine phenotypic and genetic features of strains preliminary classified as Clostridium botulinum species. The phenotypic characteristics were assessed with different culture media and biochemical tests. The genetic characterization included detection of botulinum toxin genes by PCR and macrorestriction analysis with SmaI, XhoI and SacII by PFGE (Pulsed-field Gel Electrophoresis). Despite similar biochemical properties of all analysed strains, only 47% of them contained genes determining toxicity specific to C. botulinum species. The most valuable differentiation of C. botulinum and C. botulinum-like strains was obtained after SmaI digestion. The highest affinity was observed among C. botulinum type B profiles which was even up to 100%. It was found 100% of affinity between C. botulinum and C. botulinum-like strains, however, the similarity among C. botulinum and C. botulinum-like was generally lower than 80%.

Open access

Magdalena Szenejko and Maciej Rogalski

-76. Stephens L. C., Fei S. Z., Xiong Y. & Hodges C. F. 2006. Plants regenerated from embryo cultures of an apomictic clone of Kentucky bluegrass (Poa pratensis L. ‘Baron’) are not apomictic in origin. Euphytica 147: 383-388. Szenejko M. 2010. Zróżnicowanie cech morfologicznych oraz plonu masy nadziemnej wybranych ekotypów podlaskich Poa pratensis w początkowym etapie ich rozwoju. Łąk. Pol. 13: 167-181. Szenejko M. 2012. Evaluation of the phenotypic and genomic variation of different Kentucky bluegrass (Poa pratensis L.) forms. Ph. D. Thesis

Open access

E. Puacz, W.M. Ilczyszyn, M. Kosecka, A. Buda, W. Dudziak, K. Polakowska, T. Panz, A. Białecka, A. Kasprowicz, A. Lisowski, H. Krukowski, V. Cuteri and J. Międzobrodzki

, Hryniewicz W (2008) Molecular surveillance of methicillin-resistant Staphylococcus aureus by multiple-locus variable number tandem repeat fingerprinting (formerly multiple-locus variable number tandem repeat analysis) and spa typing in a hierarchic approach. Diagn Microbiol Infect Dis 62: 255-262. Kot B, Piechota M, Wolska KM, Frankowska A, Zdunek E, Binek T, Kłopotowska E, Antosiewicz M (2012) Phenotypic and genotypic antimicrobial resistance of staphylococci from bovine milk. Pol J Vet Sci 15: 677-683. Le Maréchal C, Seyffert N, Jardin J, Hernandez D, Jan G

Open access

A. Jamalzade, M. Shamsbakhsh and H. Rahimian

Erwinia by 16s-23s intergenic transcribed spacer- PCR and restriction fragment length polymorphism analysis. Applied and Environmental Microbiology, 67, 4070-4076. Verdonck L., Mergaert J., Rijckaert G., Swings J., Kersters K., Deley J., 1987 - The genous Erwinias: numerical analysis of phenotypic features. International Journal of Systematic Bacteriology, 37, 4-18. Versalovic J., Koeuth T., Lupski J. R., 1991 - Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial

Open access

Olawe Mashood Aliyu

://www.iaabd.org/pdf/2008_IAABD_Proceedings.pdf ALIYU, O. M., O. O. ADEIGBE and O. O. LAWAL (2014): Phenotypic stability analysis of yield components in cashew (Anacardium occidentale L.) using additive main effect and multiplicative interaction (AMMI) and GGE biplot analyses. Plant Breeding and Biotechnology, 2 (4): 354-369. ARUMUGANATHAN, K. and E. D. EARLE (1991): Nuclear DNA content of some important plant species. Plant Molecular Biology Reporter 9 (3): 208-219. BENNETT, M. D. and I. J. LEITCH (2011): Nuclear DNA amounts in angiosperms

Open access

Shahram Falamarzi, Behzad Habibpour, Mohammad S. Mossadegh and Alireza Monfared

Abstract

In the present work we used landmark-based geometric morphometrics to compare the wing shapes of five species of Megachile (belonging to three subgenera) to confirm whether this technique may be used reliably for differentiation of this group. Analyses of wing shape by the use of principal component analysis (PCA), and canonical variate analysis (CVA) led to a clear differentiation among species. We found a close phenotypic similarity in wing shape between M. albisecta (belonging to the subgenus Creightonella) and M. picicornis (belonging to the subgenus Eutricharaea). According to the results of UPGMA, a higher degree of divergence between M. farinosa (belonging to the subgenus Pseudomegachile) and species belonging to other subgenera, was detected. The results of a cross-validation test indicated that geometric morphometrics is an effective technique to use for distinguishing between Megachile species. The reliability rate of this technique was between 85.71-100%. Using only two submarginal cell landmarks for generating shape variables, the cross-validation test correctly assigned individuals to their respective species, with a 92.85-100% reliability rate. Significant differences in wing size were obtained among the analysed species.

Open access

Dávid Kováts and Andrea Harnos

References Agrawal, A. A. 2001. Phenotypic plasticity in the interactions and evolution of species. – Science 294: 321–326. DOI: 10.1126/science.1060701 Amrhein, V., Kunc, H. P. & Naguib, M. 2004. Seasonal patterns of singing activity vary with time of day in the Nightingale (Luscinia megarhynchos) . – The Auk 121: 110–117. DOI: 10.1642/0004-8038(2004)121[0110:SPOSAV]2.0.CO;2 Ács, Z. & Kováts, D. 2013. Phylogenetic patterns of a nightingale population in a contact zone of Luscinia megarhynchos and L. luscinia in Hungary. – North

Open access

M. Kizerwetter-Świda, D. Chrobak-Chmiel, M. Rzewuska, A. Antosiewicz, B. Dolka, A. Ledwoń, A. Czujkowska and M. Binek

developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41: 1574-1585. Parte AC (2014) LPSN – list of prokaryotic names with standing in nomenclature. Nucleic Acids Res 42: D613-D616. Sasaki T, Kikuchi K, Tanaka Y, Takahashi N, Kamata S, Hiramatasu K ( 2007 ) Reclassification of phenotypically identified Staphylococcus intermedius strains. J Clin Microbiol 45: 2770-2778. Sasaki T, Tsubakishita S, Tanaka Y, Sakusabe A, Ohtsuka M, Hirotaki S, Kawakami T, Fukata T, Hiramatsu K

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Zoya Khan, Wan Maznah Wan Omar, Faradina Merican Mohd Sidik Merican, Asmimie Asmawarnie Azizan, Choon Pin Foong, Peter Convey, Nazalan Najimudin, Jerzy Smykla and Siti Aisyah Alias

References A cinas S.G., H averkamp T.H.A., H uisman J. and S tal L.J. 2009. Phenotypic and genetic diversification of Pseudanabaena spp. (cyanobacteria). ISME Journal 3: 31–46. A nagnostidis K. and K omárek J. 1988. Modern approach to the classification system of cyanophytes. 5. Oscillatoriales. Archiv für Hydrobiologie 80: 327–472. A ndersen R.A. 2005. Algal Culturing Techniques . Elsevier Academic Press: 92–94. B oyer S.L., F lechtner V.R. and J ohansen J.R. 2001. Is the 16S-23S rRNA internal transcribed spacer region a