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Investigation on Canine parvovirus circulation in dogs from Sicily (Italy) by biomolecular assay

parvovirus type 2c in South America. Vet Microbiol 2007, 124:147–152. 5. Reed AP, Jones EV, Miller TJ: Nucleotide sequence and genome organization of canine parvovirus. J Virol 1988, 62:266-276. 6. Binn LN, Lazar EC, Eddy GA, Kajima M: Recovery and characterization of a minute virus of canines. Infect Immun 1970, 1:503-508. 7. Battilani M, Ciulli S, Tisato E, Prosperi S: Genetic analysis of canine parvovirus isolates (CPV-2) from dogs in Italy. Virus Res 2002, 83:149-157. 8. Berns KI, Bergoin M, Bloom M, Muzyczka N, Tal J, Tattersall P: Family

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Diagnostics and genotyping of Canine parvovirus type 2 (CPV-2) from disease cases in south-eastern Poland

REFERENCES 1. Patial S, Chaturvedi VK, Rai A, Saini M, Chandra R, Saini Y, Gupta PK:. Virus neutralizing antibody response in mice and dogs with a bicistronic DNA vaccine encoding rabies virus glycoprotein and canine parvovirus VP2. Vaccine 2007, 25:4020-4028. 2. Xu J, Guo HC, Wei YQ, Shu L, Wang J, Li JS, Cao SZ, Sun SQ: Phylogenetic analysis of canine parvovirus isolates from Sichuan and Gansu provinces of China in 2011. Transbound Emerg Dis 2015, 62:91-95. 3. Decaro N, Buonavoglia C: Canine parvovirus-A review of epidemiological and

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Molecular Detection of Pseudorabies Virus (PrV), Porcine Parvovirus (PPV) and Porcine Circovirus 2 (PCV2) in Swine in Republic of Montenegro

hunting dogs. Veterinary Microbiology 2012, 157:276-284. 6. Huang C, Hung J, Wu C, Chien M: Multiplex PCR for rapid detection of pseudorabies virus, porcine parvovirus and porcine circoviruses. Veterinary Microbiology 2004, 101: 209-214. 7. Ogawa H, Taira O, Hirai T, Takeuchi H, Nagao A, Ishikawa Y, Tuchiya K, Nunoya T, Ueda S: Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and RNA viruses in pigs with multiple infections. Journal of Virological Methods 2009, 160:210-214. 8. Cadar D, Dan A, Tombacz K, Lorincz M, Kiss T

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Serological and Molecular Biological Studies of Parvovirus B19, Coxsackie B Viruses, and Adenoviruses as Potential Cardiotropic Viruses in Bulgaria

Abstract

Background: Inflammatory diseases of the heart (myocarditis, pericarditis) are commonly caused by viruses. Among the human cardiotropic viruses, parvovirus B19, Coxsackie B viruses, and adenoviruses play a leading role.

Aim: The aim of the present study was to determine the presumptive causative role of parvovirus B19, Coxsackie B viruses, and adenoviruses in the development of myocarditis, pericarditis and dilated cardiomyopathy by demonstrating the presence of specific antiviral antibodies or viral DNA in patients’ serum samples.

Materials and methods: We tested serum samples collected between 2010 and 2014 from 235 patients with myocarditis (n=108), pericarditis (n=79), myopericarditis (n=19), dilated cardiomyopathy (n=7), and fever of unknown origin accompanied by cardiac complaints (n=22). The mean age of patients with the standard deviation was 33 ± 18 years. Serological and molecular methods (ELISA for specific IgM/IgG antibodies to parvovirus B19 and IgM antibodies to Coxsackie B viruses and adenoviruses, and PCR for detection of parvovirus B19 in serum samples, respectively) were used in the study.

Results: Of all tested 235 serum samples, in 60 (25.5%) positive results for at least one of the three tested viruses were detected. Forty out of these 235 serum samples (17%) were Coxsackie B virus IgM positive. They were found in 17% (18/108) of the patients with myocarditis, in 15% (12/79) of those with pericarditis, in 16% (3/19) of those with myopericarditis and in 32% (7/22) in those with fever of unknown origin. The 63 Coxsackie B virus IgM negative patient’s serum samples were tested by ELISA for presence of adenovirus IgM antibodies. Such were found in 4 patients with pericarditis and in 2 patients with fever of unknown origin. Every IgM negative sample (n=189) for Coxsackie B and adenovirus was further tested by ELISA for parvovirus B19 IgM/IgG antibodies. B19-IgM antibodies were detected in 14 patients (7.4%). The percentages for B19-IgM antibodies was 8% (7/90), 5% (3/63) and 31% (4/13) in the patients affected with myocarditis, pericarditis, and fever of unknown origin, respectively. Protective B19-IgG antibodies were found in 108 (57%) of the samples. A B19-PCR signal was detected in all the patients who were B19-IgM positive, and in only 1 patient with positive B19-IgG result, the latter presenting with dilated cardiomyopathy.

Conclusion: The present study shows the involvement of Coxsackie B, parvovirus B19 and adenoviruses in the development of inflammatory diseases of the heart (myocarditis and pericarditis). It is the first ever study in the country that simultaneously analyzes the prevalence of the three major human cardiotropic viruses.

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Clinical Case of Aplastic Crisis Associated with Extramedullary Hematopoiesis in an Adult With Hereditary Spherocytosis and Parvovirus B19 Infection

References 1. P. H. B. Bolton-Maggs, R. F. Stevens, N. J. Dodd, G. Lamont, P. Tittensor and M. J. King, “Guidelines for the diagnosis and management of hereditary spherocytosis”, British Journal of Haematology, 126: 455-474, 2004. 2. N. S. Young, K. Brown “Mecanism of disease Parvovirus B19”, NEJM, 350:586-97, 2004. 3. H. Mulder, J.T.Schlangen, A.E.vanVoorthuisen,“Extramedullary hematopoiesis in the posterior mediastinum”, RadiolClin (Basel), 44(6):550-6, 1975. 4. J.J. Petit, C. Estany

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Loop-Mediated Isothermal Amplification as a Simple Molecular Method for the Detection of Derzsy’s Disease Virus

References 1. Brown K.E., Green S.W., Young N.S.: Goose parvovirus - an autonomus member of the Dependovirus genus. Virology 1995, 210 , 283-291. 2. Chu C.Y., Pan M.J., Cheng J.T.: Genetic variation of the nucleocapsid genes of waterfowl parvovirus. J Vet Med Sci 2001, 63 , 1165-1170. 3. Gough R.E.: Application of the agar gel precipitation and virus neutralization test to the serological study of goose parvovirus. Avian Pathol 1984, 13 , 501-509. 4. Huang C., Cheng A.C, Wang M.S., Liu

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Synovitis in Osteoarthritic Patients: Morphological and Virological Evidence of its Contribution to Development of the Disease

REFERENCES Adamson-Small, L. A., Ignatovich, I. V., Laemmerhirt, M. G., Hobbs, J. A. (2014). Persistent parvovirus B19 infection in non-erythroid tissues: Possible role in the inflammatory and disease process. Virus Res ., 190 , 8–16. Altman, R., Asch, E., Bloch, D., Bole, G., Borenstein, D., Brandt, K., Christy, W. (1986). Development of criteria for the classification and reporting of osteoarthritis classification of osteoarthritis of the knee. Arthr. Rheum ., 29 , 1039–1049. Altman, R., Howell, D., Ike, R., Kapila, P., Kaplan, D., Koopman

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Molecular Detection of PCV2 And PPV in Pigs in Republic of Srpska, Bosnia and Herzegovina

Hungarica 2010, 58:483-498. 10. Cadar D, Dan A, Tombacz K, Lorincz M, Kiss T, Becskei Z, Spinua M, Tuboly T, Csagola A: Phylogeny and evolutionary genetics of porcine parvovirus in wild boars. Infect Genet Evol 2012, 12:1163-1171. 11. Dias AS, Gerber PF, Araujo AS, Auler PA, Gallinari GC, Lobato ZIP: Lack of antibody protection against Porcine circovirus 2 and Porcine parvovirus in naturally infected dams and their offspring. Res Vet Sci 2013, 94:341-345. 12. Chen HY, Li XK, Cui BA, Wei ZY, Li XS, Wang YB, Zhai L, Wang ZY: A

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Cytokines and MMP-9 Levels in Rheumatoid Arthritis and Osteoarthritis Patients with Persistent Parvovirus B19, HHV-6 and HHV-7 Infection

., Emekdas, G. (1988). Detection of parvovirus B19 in synovial fluids of patients with osteoarthritis. Diagn. Microbiol. Infect. Dis ., 60 (4), 381–385. Barah, F., Vallely, P., Chiswick, M., Cleator, M., Kerr, J. (2001). Association of human parvovirus B19 infection with acute meningoencephalitis. Lancet , 358 , 729–730. Barash, J., Dushnitzki, D., Barak, Y., Miron, S., Hahn, T. (2003). Tumor necrosis factor (TNF) alpha and its soluble receptor (sTNFR) p75 during acute human parvovirus B19 infection in children. Immunol. Lett ., 88 , 109–112. Beal

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Peripheral Blood Mononuclear Cells’ Proliferative Response to Human Parvovirus B19 Antigens in Patients with Rheumatoid Arthritis / Reimatoīdā Artrīta Slimnieku Perifēro Asiņu Mononukleāro Šūnu Proliferativā Atbilde Uz Cilvēka Parvovīrusa B19 Antigēniem

Classification Criteria. Arthritis Rheum., 62 (9), 2569-2581. Arnett, F. C., Edworthy, S. M., Bloch, D. A., McShane, D. J., Fries, J. F., Cooper, N. S., Healey, L. A., Kaplan, S. R., Liang, M. H., Luthra, H. S. (1988). The American Rheumatism Association 1987 revised criteria for the rheumatoid arthritis. Arthritis Rheum., 31 (3), 315-324. Barah, F., Vallely, P. J., Chiswick, M. L., Cleator, G. M., Kerr, J. R. (2001). Association of human parvovirus B19 infection with acute meningoencephalitis. Lancet, 358, 729-730. Broliden, K

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