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Sanja Goč and Miroslava Janković

; 57: 921-7. 20. Kosanović M, Goč S, Potpara G, Janković M. On chip immuno-affinity profiling of cancer- and benign hyperplasia- associated free prostate specific antigen. Dis Markers 2011; 31: 111-8. 21. Janković M. Glycans as biomarkers: status and perspectives. J Med Biochem 2011; 30: 213-23. 22. Patrie SM, Roth MJ, Zhang J. Methods in molecular biology: mass spectrometry of glycoproteins. Top-down mass spectrometry for protein molecular diagnostics and biomarker discovery, In: Issaq HJ, editor. Proteomics and

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Savelina L. Popovska, Tereza B. Dineva and Polina D. Damyanova

. Vanderbilt-Ingram Cancer Center. Molecular Profiling of Lung Cancer [Internet]. Nashville; c2016. [cited 2018 Febr 18]. Available from: . 18. Huang L, Fu L. Mechanisms of resistance to EGFR tyrosine kinase inhibitors. Acta Pharm Sin B. 2015;5(5):390-401. 19. Yu HA, Arcila ME, Rekhtman N, Sima CS, Zakowski MF, Pao W, et al. Analysis of tumor specimens at the time of acquired resistance to EGFR-TKI therapy in 155 patients with EGFR-mutant lung cancers. Clin Cancer Res. 2013;19(8):2240-7. 20

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Martina Rebersek, Tanja Mesti, Marko Boc and Janja Ocvirk

. Biomarker analysis DNA for molecular analysis was extracted from formalin-fixed, paraffin-embedded tumour tissue of primary tumours or metastases with at least 70% of tumour cells. TheraScreen KRAS Mutation Kit ® (Roche Applied Science, Mannheim, D) was used to determine seven most common mutations in codons 12 and 13 of the KRAS gene. The V600E mutation in BRAF was detected by end-point genotyping using the TaqMan MGB probes (Applied Biosystems, Warrington, UK) as described previously. 16 The mutation V600E in BRAF in positive tumour samples was confirmed by

Open access

Artūras Serackis, Dalius Matuzevičius, Dalius Navakauskas, Eldar Šabanovič, Andrius Katkevičius and Darius Plonis


The aim of the investigation presented in this paper was to develop a software-based assistant for the protein analysis workflow. The prior characterization of the unknown protein in two-dimensional electrophoresis gel images is performed according to the molecular weight and isoelectric point of each protein spot estimated from the gel image before further sequence analysis by mass spectrometry. The paper presents a method for automatic and robust identification of the protein standard band in a two-dimensional gel image. In addition, the method introduces the identification of the positions of the markers, prepared by using pre-selected proteins with known molecular mass. The robustness of the method was achieved by using special validation rules in the proposed original algorithms. In addition, a self-organizing map-based decision support algorithm is proposed, which takes Gabor coefficients as image features and searches for the differences in preselected vertical image bars. The experimental investigation proved the good performance of the new algorithms included into the proposed method. The detection of the protein standard markers works without modification of algorithm parameters on two-dimensional gel images obtained by using different staining and destaining procedures, which results in different average levels of intensity in the images.

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Roxana Buzas, Alexandru Florin Rogobete, Sonia Elena Popovici, Tudor Mateescu, Teodora Hoinoiu, Virgiliu-Bogdan Sorop, Tiberiu Bratu, Marian Ticlea, Calin Marius Popoiu and Dorel Sandesc

Expression as Genetic Biomarkers in Acute Cardiac Care. Journal of Cardiovascular Emergencies. 2016;2:169-172. doi: 10.1515/jce-2016-0025. 46. Papurica M, Rogobete AF, Sandesc D, et al. Using the Expression of Damage-Associated Molecular Pattern (DAMP) for the Evaluation and Monitoring of the Critically Ill Polytrauma Patient. Clin Lab. 2016;62:1829-1840. doi: 10.7754/Clin.Lab.2016.160226. 47. Sandesc M, Dinu A, Rogobete AF, et al. Circulating microRNAs expressions as genetic biomarkers in pancreatic cancer patients continuous non-invasive monitoring. Clin Lab

Open access

M. Shahid, G. Manchi, P. Slunsky, O. Naseer, A. Fatima, B. Leo and J. Raila

-960. Kosinska MK, Ludwig TE, Liebisch G, Zhang R, Siebert HC, Wilhelm J, Kaesser U, Dettmeyer RB, Klein H, Ishaque B (2015) Articular joint lubricants during osteoarthritis and rheumatoid arthritis display altered levels and molecular species. PLoS One 10: e0125192. Kraus VB, Burnett B, Coindreau J, Cottrell S, Eyre D, Gendreau M, Gardiner J, Garnero P, Hardin J, Henrotin Y (2011) Application of biomarkers in the development of drugs intended for the treatment of osteoarthritis. Osteoarthritis Cartilage 19: 515-542. Li Z, Cao L, Wang S (2009

Open access

Jana Kovářová and Zdeňka Svobodová

Toxicol   80 : 298-308. Santovito G, Irato P, Piccinni E, Albergoni V. (2000). Relationship between metallothionein and metal contants in red-blooded and white-blooded Antarctic teleost. Polar Biol   23 : 383-391. Sarkar A, Ray D, Shrivastava AN, Sarker S. (2006). Molecular biomarkers: their significance and application in marine pollution monitoring. Ecotox   15 : 333-340. Schlenk D and Rice CD. (1998). Effect of zinc and cadmium treatment on hydrogen-peroxide induced mortality and

Open access

Martin Pejchinovski and Harald Mischak

Proteomics. 2011; 10(3) 59. Fechete R, Heinzel A, Perco P et al. Mapping of molecular pathways, biomarkers and drug targets for diabetic nephropathy. Proteomics Clinical Applications. 2011; 5(5-6): 354-366. 60. Krochmal M, Fernandes M, Filip S et al. PeptiCKDdb- peptide- and protein-centric database for the investigation of genesis and progression of chronic kidney disease. Database-the Journal of Biological Databases and Curation. 2016; 61. Dominiczak AF, Herget-Rosenthal S, Delles C et al. Systems biology to battle vascular disease. Nephrol Dial

Open access

Elena Dantes, Doina Ecaterina Tofolean, Ariadna Petronela Fildan, Laura Mazilu, Ioana Gogonea, S. Dumitrache-Rujinski and Oana Cristina Arghir

-586. . 10. Sequist, L.V., Neal, W.N. (2017). Personalized, genotype-directed therapy for advanced nonsmall cell lung cancer. UpToDate 2017 11. Kerr, K.M., Bubendorf, L., Edelman, M.J., Marchetti, A., Mok, T., Novello, S., O'Byrne, K., Stahel, R., Peters, S. & Felip, E. (2014). Second ESMO consensus conference on lung cancer: pathology and molecular biomarkers for non-small-cell lung cancer. Annals of Oncology, 25(9), 1681-1690. doi: 10.1093/annonc/mdu145 12. Leighl, N.B., Rekhtman, N., Biermann, W.A., Huang, J., Mino-Kenudson, M

Open access

NT Popov, DS Minchev, MM Naydenov, IN Minkov and TI Vachev

patterns as potential serum-based biomarkers for ASD diagnosis is still in its infancy. The question of how exactly miRNAs regulate their target genes through fine molecular mechanisms in the context of ASD pathogenesis are not fully understood. It is currently known that individual miRNAs can have several target genes, and thus, have an impact on more than one pathway. It was found that miRNAs can regulate translation of a wide range of proteins in neurons [ 15 ] including proteins involved in neuronal migration [ 16 ], channels [ 17 ] and neuronal morphology [ 18 ]. A