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Open access

Zhifu Yin and Helin Zou

R eferences [1] X. Zhang, L. Li and C. Luo, “Gel integration for microfluidic applications”, Lab on a Chip , vol. 16, no.10, pp. 1757-1776, 2016. [2] B. C. Lin, “Research and Industrialization of Microfluidic Chip”, Chinese Journal of Analytical Chemistry , vol. 44, no.4, pp. 491-499, 2016. [3] L. Wang, W. Liu, S. Li, T. Liu, X. Yan, Y. Shi, Z. Cheng, C. And and Chen, “Fast fabrication of microfluidic devices using a low-cost prototyping method”, Microsystem Technologies-Micro-and Nanosystems-Information Storage and Processing Systems , vol

Open access

Karol Malecha

References Gravesen, P., Branebjerg, J., Søndergård Jensen, O. (1993). Microfluidic - a review. J. Micromech. Microeng. , (3), 168-182. Wautelet, M. (2001). Scaling laws in the macro-, micro- and nanoworlds. European Journal of Physics , (22), 601-611. Manz, A., Graber, N., Widmem, H. M. (1990). Miniaturized total chemical analysis system: a novel concept for chemical sensing. Sens. Actuators B , (1), 244-248. Duffy, D. C., Cooper McDonald, J., Schueller

Open access

Mihǎiţǎ Nicolae Ardeleanu, Simona Mihai, Ruxandra Vidu, Emil Mihai Diaconu and Ileana Nicoleta Popescu

REFERENCES [1] Zhou, Y., Basu, S., Wohlfahrt, K. J., Lee, S. F., Klenerman, D., Laue, E. D., & Seshia, A. A., A microfluidic platform for trapping, releasing and super-resolution imaging of single cells. Sensors and Actuators B: Chemical, 232 (2016) 680-691. [2] Ravetto, A., Hoefer, I. E., den Toonder, J. M., & Bouten, C. V., A membrane-based microfluidic device for mechano-chemical cell manipulation. Biomedical microdevices, 18(2) (2016) 31. [3] Van Dam, R. Michael, Solvent-resistant elastomeric microfluidic devices and applications

Open access

R.S.R. Gorla and B.J. Gireesha

References Abbassi H. (2007): Entropy generation analysis in a uniformly heated microchannel heat sink . – Energy, vol.32, pp.1932–1947. Bejan A. (1996): Entropy Generation Minimization . – New York: CRC Press. Chen K. (2005): Second law analysis and optimization of microchannel flows subject to different boundary conditions. – Int. J. Energy Res., vol.29, pp.249–263. ChulJin Choi, Seok Pil Jang and Stephen U.S. Choi (2011): Electrokinetic effects of charged nanoparticles in microfluidic Couette flow . – Journal of Colloid and

Open access

M Vazan, I Kasubova, A Vanochova, P Lukac, L Plank and Z Lasabova

Abstract

Introduction: A clonal population of B-cells is defined as those cells arising from the mitotic division of a single somatic cell with the same rearrangement of immunoglobulin genes. This gives rise to DNA markers for each individual lymphoid cell and its progenies and enables us to study clonality in different B-cell malignancies using multiplex polymerase chain reaction - PCR. The BIOMED-2 protocol has been implemented for clonality detection in lymphoproliferative diseases and exploits multiplex PCR reaction, subsequently analyzed by heteroduplex analysis (HDA) using polyacrylamide gel electrophoresis (PAGE). With the advent of miniaturization and automation of molecular biology methods, lab-on-chip technologies were developed and replace partially the conventional approaches. We tested device for microfluidic chip, which is used for B-cells clonality analysis, using a PCR reaction for three subregions called frameworks (FR) of the immunoglobulin heavy locus (IGH) gene.

Material and Methods: For the implementation and comparison of the two methods we analyzed three unknown B-cell chronic lymphocytic leukemia (B-CLL) samples. As positive control (PK) we used one formalin-fixed, paraffin-embedded (FFPE) sample from B-CLL lymph node. The DNA was extracted from FFPE sections and multiplex PCR was used to amplify IGH gene segments. After PCR, the HDA was performed, the DNA fragments were evaluated on the PAGE and the microfluidic chip electrophoresis as well, and the results were compared.

Results: Using HDA with subsequent PAGE, we were able to confirm the clonality of the positive control and the tested samples. The same results were obtained by the Bioanalyzer 2100. The microfluidic chip electrophoresis was persuasive in all tested samples.

Conclusion: The implementation of microfluidic chip electrophoresis for detection of B-cell clonality by BIOMED-2 protocol on the device Agilent 2100 Bioanalyzer was successful and yielded the same results as the HDA - PAGE. Moreover, chip electrophoresis system is faster for preparation and less laborious than the conventional HDA - PAGE method.

Open access

Afia Asif, Saed Khawaldeh, Muhammad Salman Khan and Ahmet Tekin

square of the built device, and surely the lengths to cross are much smaller. This characteristic was presented lately in co-flow trigonometry, employing plug flow in capillaries or droplets, and also it has been applied in droplets which are confined in micro-chambers. Several former studies in the past decades have described different implementation methods and usage of microfluidics devices. In 2013, David J. Beebe et al . [ 1 ] developed an enclosing abstraction for microscale capillary flow. This developed concept was called suspended microfluidics. Suspended

Open access

Karolina Blaszczyk, Michal Chudy, Zbigniew Brzozka and Artur Dybko

on human carcinoma cells in microfluidic system, Sens Actuators B, , 160, 1544-1551 [4] Jedrych E., Pawlicka Z., Chudy M., Dybko A., Brzozka Z., (2011), "Evaluation of photodynamic therapy (PDT) procedures using microfluidic system" Analytica Chimica Acta , 683, 149-155 [5] Ziolkowska, K., Jedrych, E., Kwapiszewski, R., Lopacinska, J., Skolimowski, M., Chudy M., (2010), PDMS/glass microfluidic cell culture system for cytotoxicity tests and cells passage, Sensors and Actuators B 145, 533-542 [6] Therry, S

Open access

V. Novickij, A. Grainys and J. Novickij

The microfluidic channel with a planar inductive microcoil for the cell membrane permeabilization and the integrated planar electrodes for cell dielectrophoretic manipulation is proposed and analyzed in the study. The analyzed setup is based on the dielectrophoretic entrapment of the biological cell followed by membrane permeabilization using high pulsed magnetic field. The finite element method analysis of the DEP force and the generated pulsed magnetic field is performed. Based on finite element method analysis the potential applications of the setup in the fields of drug delivery, biomedicine and biotechnology are discussed.

Open access

Muhammad Salman Khan, Afia Asif, Saed Khawaldeh and Ahmet Tekin

cerevisiae cells at pH 7.2, the obtained value of R(ct) showed over 560 percent from the value obtained on the same thiol-modified electrode [ 8 , 9 , 10 , 11 , 12 ]. The main objective of this presented work is to give a better understanding of the comparative study of targeted dopamine detection with two modifications for the surface of Au electrodes i.e., (1) cysteamine and (2) MPA for thermally bonded and ultrasonically welded microfluidic chips, respectively. The influence of both bonding techniques along with the selection of optimized tubing for the fabricated

Open access

David Cheneler, James Bowen and Georgia Kaklamani

mechanotransduction are explored using viable artificial tissue-engineered skin in the form of an alginate encapsulated fibroblast. The artificial skin, which is considered to be a model system, is kept viable via a bespoke microfluidic system with integrated coplanar impedance sensors. Accurate normal loads are applied to the exposed surface of the artificial skin via indentation at small and large strains and the impedance monitored in real-time at a fixed single frequency. The fabrication of the microfluidic system and impedance sensors is described. The electrical properties of