Andrei Crauciuc, Florin Tripon, Andreea Gheorghiu, Georgiana Nemes, Alina Boglis and Claudia Banescu
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Alina Martinescu, Irina Franciuc and Loredana-Mariana Aftenie
Genome scan, linkage and association studies have identified CMH genes in the genetic determinism of most autoimmune diseases, affecting approximately 5% of the population. The susceptibility conferred by HLA alleles does not influence the development of autoimmunity in general, but rather the probability of some disorders. From extremely high number of known HLA alleles, less than 30 are associated with diseases, and fewer than 10 are involved in the strongest associations. The aim of this study was to determine HLA genes in autoimmune diseases. The method used for the assignment of HLA alleles was molecular genotyping, primarily by the sequence specific oligonucleotide hybridization method (SSO), and when required, by the sequence specific primers method (SSP). Molecular genotyping was performed in 282 cases of diabetes mellitus type 1, Hashimoto thyroiditis, Graves’ disease and ankylosing spondylitis. HLA typing in patients with autoimmune diseases is important for determining prognosis and genetic counseling. In ankylosing spondylitis HLA typing is used for differential diagnosis. In such studies it’s important to be aware that there is a particular HLA gene expression depending on the geographic area.
This overview is an attempt to throw a fresh look at the popular free radical theory of aging (referred to also as oxidative stress theory) which holds that the progressive decline in physiological functions is a result of accumulation of diverse deleterious changes caused by reactive oxygen species (ROS). We discuss the role of mitochondria as a major source of ROS in the cell and how these link accumulation of oxidative damage to the age-related changes in physiologic functions. The free radical theory of aging is analysed here from two different views of aging - one (the pessimistic view) that regards aging as the inevitable result of life activity the consequences of which are accumulation of errors in the genome and damage of the biomolecules, and the other (the optimistic view) which considers that it is the changes in mitochondrial pathways of apoptosis with age that cause the functional tissue changes and aging.
We also discuss the possibility of delaying the aging process by appropriate diet or drug therapy, which includes also calorie restriction as a mechanism of modifying the generation of free radicals and body metabolism and thus extending lifespan as a result.
Tatyana M. Kichukova, Nikolay T. Popov, Ivan S. Ivanov and Tihomir I. Vachev
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Hristo Y. Ivanov, Vili K. Stoyanova, Nikolay T. Popov and Tihomir I. Vachev
quantitative trait locus on chromosome 7q in multiplex autism families. Am J Hum Genet 2002;70:60-71.
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Aliz-Beáta Tunyogi, I Benedek, Judit Beáta Köpeczi, Erzsébet Benedek, Enikő Kakucs, Monica Istrati and Zsuzsa Pap
decades of transplantation for chronic myeloid leukemia: what have we learned? Blood. 2011;117:755-763.
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systematic genome detection in cerebrospinal fluid irrespective of cytologic examination results. Journal of clinical virology. 21(1), 29-35.
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