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Katarzyna Andraszek, Dorota Banaszewska, Małgorzata Szostek, Ewa Wójcik, Magdalena Czubaszek and Renata Walczak-Jędrzejowska

Abstract

The study was conducted on spermatozoa of selected farm and free-living animal species, isolated post mortem from the tail of the epididymis, and stained with silver nitrate - AgNO3. The material was collected from pigs, goats, wild boar, and European roe deer. Twenty morphologically normal spermatozoa randomly selected from each animal and well visible under the microscope, were analysed. The following measurements were considered: head length, width, perimeter and area, acrosome area, mid-piece length, tail length, and overall sperm length. AgNO3 staining differentiated the acrosomal (light hue) and distal (dark hue) part of the sperm head, and a light-hued mid-piece was visible within the sperm tail. Silver nitrate staining revealed species and variety-related differences, particularly in reference to the sperm head. Clear-cut differentiation within the head and tail area made it possible to perform detailed morphometric measurements of the spermatozoa.

Open access

K. Syczyło, A. Platt-Samoraj, A. Bancerz-Kisiel, A. Szczerba-Turek, K. Lipczyńska, A. Jabłoński, Z. Procajło and W. Szweda

Abstract

The aim of the study was to monitor Y. enterocolitica strains from free-living animals captured during 2011-2014 hunting seasons in Poland using warm (ITC) and cold (PSB) enrichment and molecular examination. Over 1600 samples have been cultured. After ITC/PSB enrichment 237 strains presenting features characteristic for Y. enterocolitica were isolated. Molecular examination using multiplex PCR revealed 140 isolates from PSB and 78 from ITC. The concentration of pathogenic Yersinia in asymptomatic carriers is low and the PCR detection should be preceded by bacteriological examination.

Open access

Tadeusz Kośla, Michał Skibniewski and Ewa M. Skibniewska

. Importance of sulphur in agriculture. Chemia Dydaktyka Ekologia Metrologia. 14: 69-78. KOŚLA T., SKIBNIEWSKA E.M., SKIBNIEWSKI M. 2011. The state of bioelements in the hair of free-ranging European bisons from Białowieża primeval forest. Polish J. Vet. Sci 14: 81-86. KOŚLA T., SKIBNIEWSKA E.M., URBAŃSKA-SŁOMKA G., SKIBNIEWSKI M. 2007. Correlation between sulphur content in hair and the results in blood of haematological and biochemical examinations in cats free living in Warsaw. Environmental Protection and Natural Resources 31: 438-441. LAMAND M

Open access

Jakubczak Andrzej, Gryzinska Magdalena, Horecka Beata, Dziadosz Katarzyna and Jezewska-Witkowska Grazyna

, Piórkowska M: Genetic variability of farmed and free-living populations of red foxes [Vulpes vulpes]. Annals of Animal Science 2012, 12:501-512. 29. Nowakowicz-Dębek B, Zoń A, Jakubczak A, Wnuk W: Hematological parameters of wild and farm mink, red fox and raccoon dog. Med Wet 2013. 69[1]:40-42. 30. Sacks BN, Louie S: Using the dog genome to fi nd single polymorphisms in red foxes and other distantly related members of the Canidae. Mol Ecol Resour, 2008, 8:35-49.

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Ewa M. Skibniewska, Michał Skibniewski and Tadeusz Kośla

in the tissues of free-ranging European bison.Magnesium Research. 25: (2), 99-103. KOŚLA T., SKIBNIEWSKI M., SKIBNIEWSKA E., URBAŃSKA- SŁOMKA G. 2004. The zinc status in free living European bisons. Acta Alimentaria 33: (3), 269-273. MUKHERJEE B., PATRA B., MAHAPATRA S., BANERJEE P. , TIWARI A., CHATTERJEE M. 2004.Vanadium- an element of atypical biological significance Toxicology Letters 150: 135-143. NRIAGU J.O. 1998. Vanadium in the Environment. John Wiley and Sons, New York. PYRZYNSKA K., WIERZBICKI T. 2004. Determination of

Open access

Michał Skibniewski, Tadeusz Kośla and Ewa M. Skibniewska

depending on their keeping conditions. Bulletin of the Veterinary Institute in Pulawy 55: (1), 149-153. KOŚLA T., SKIBNIEWSKA E.M., SKIBNIEWSKI M., URBAŃSKA-SŁOMKA G. 2012. Magnesium concentrations in the tissues of free-ranging European bison. Magnesium Research. 25: (2), 99-103. KOŚLA T., SKIBNIEWSKI M., SKIBNIEWSKA E., URBAŃSKA-SŁOMKA G. 2004. The zinc status in free living European bisons. Acta Alimentaria 33: (3), 269-273. KRZYWY I., KRZYWY E., PASTUSZAK-GABINOWSKA M., BRODKIEWICZ A. 2010. Ołów- czy jest się czego obawiać? Roczniki

Open access

B. Pilarczyk, R. Pilarczyk, A. Tomza-Marciniak, D. Hendzel, M. Bąkowska and T. Stankiewicz

concentration in liver and kidney of free living animals (roe and red deer) from West Pomerania (Poland). Eur J Wildl Res 55: 279-283. Pilarczyk B, Hendzel D, Pilarczyk R, Tomza-Marciniak A, Błaszczyk B, Dąbrowska-Wieczorek M, Bąkowska M, Adamowicz E, Bujak T ( 2010a ) Liver and kidney concentrations of selenium in wild boars (Sus scrofa) from northwestern Poland. Eur J Wildl Res 56: 797-802. Pilarczyk B, Tomza-Marciniak A, Pilarczyk R, Hendzel D, Błaszczyk B, Bąkowska M ( 2010b ) Tissue Distribution of Selenium and Effect

Open access

V. Sergi, G. Romeo, M. Serafini, E. Torretta and F. Macchioni

References A grimi , P., M ancianti , F., P oli , A. (1981): Osservazioni sulla patologia spontanea di lepri ( Lepus europaeus ) allo stato libero. Considerazioni sull’efficacia di misure di profilassi sanitaria e di trattamenti immunizzanti e terapeutici in gruppi colpiti da pasteurellosi e parassitosi intestinali [Observation on pathologies of free living hares ( Lepus europaeus ). Remarks on the efficacy of sanitary prophilaxisis and immunizing and therapeutic treatments in groups affected by Pasteurellosis and intestinal parasitosis]. Annali della

Open access

Julia Kęsik-Maliszewska, Artur Jabłoński and Magdalena Larska

–2012. UIGB congress, 2013, Bruxelles, Belgium. 7. Deutscher Jagdverband, Jagdstatistik. https://www.jagdverband.de/node/3304 . 8. European Food Safety Authority: “Schmallenberg” virus: analysis of the epidemiological data. (May 2013) Supporting Publications 2013, p. 22. www.efsa.europa.eu/publications . 9. Fenati M., Armaroli E., Corrain R., Guberti V.: Indirect estimation of porcine parvovirus maternal immunity decay in free-living wild boar ( Sus scrofa ) piglets by capture-recapture data. Vet J 2009, 180, 262–264. 10. Grochowska M., Larska M

Open access

Justyna Miłek and Katarzyna Blicharz-Domańska

inoculated into specific-pathogen-free chickens caused nephritis and high mortality, in contrast to H120-like IBV which revealed itself to be apathogenic. These findings indicate the possible role of these birds as asymptomatic carriers of IBV and the possibility of virus transmission to susceptible chicken populations. Similar conclusions were drawn from Chinese and Brazilian studies which showed the presence of Mass-like IBV strains in wild peafowl and pigeons ( 16 , 38 ). The detection of virus closely related to the H120 IBV vaccine strain in faeces of free living