). Fatty acids in pollen: a review of their importance for honeybees. Bee World , 82 , 60-75. http://doi.org/10.1080/0005772X.2001.11099504 Mondal, A.K., Pauri, S., & Mandal, S. (1998). Biochemical analysis of four species of Cassia L. pollen. Aerobiologia , 14 , 45-50. Moore, P.D., Webb, J.A., & Collinson, M.E. (1991). Pollen analysis , second edition, London: Black-well Scientific Publications. Nozkova, J., Fatrcová-Sramkova, K., Mariassyova, M., & Kropkova, Z. (2009). Polyphenols and antioxidant activity of bee pollen. Potravinárstvo , 3 (2
Ekta Chaudhary, Prabhawati Tiwari and Prem Lal Uniyal
Inga Zinicovscaia, Liudmila Rudi, Ana Valuta, Liliana Cepoi, Konstantin Vergel, Marina V. Frontasyeva, Alexey Safonov, Markus Wells and Dmitrii Grozdov
The cyanobacterium Nostoc linckia was used to study the biotechnology of selenium nanoparticles synthesis for the first time. The experimental conditions of the nanoparticle production by the studied cyanobacteria in aqueous cobalt selenite solutions were examined. Neutron activation analysis allowed characterization of the dynamics of accumulation of the total selenium quantity by Nostoc linckia. Scanning Electron Microscope images demonstrated extracellular formation of amorphous nanoparticles. Released selenium nanoparticles ranged in size from 10 to 80 nm. The changes of essential parameters of biomass (proteins, lipids, carbohydrates, and phycobilin) content during the nanoparticle formation were assessed. During the first 24 h of nanoparticle synthesis, a slight decline of proteins, lipids and carbohydrates content in the biomass was observed. The most extensive was the process of phycobilin degradation. Furthermore, all biochemical component content as well as an antioxidant activity of the biomass extracts significantly decreased. The obtained substance of Nostoc biomass with selenium nanoparticles may be used for medical, pharmaceutical and technological purposes.
R.E. Muir and M. Halán
Tarantulas are a relatively unstudied category of invertebrate which are popular with hobbyists and increasingly used in laboratory research. As their presence in the veterinary setting is limited, very little is known about the biochemistry of their haemolymph as obtained by in house sampling and analysis. A handful of studies have been performed to attempt to establish a normal range for certain parameters in healthy members of a few particular species, but that is the extent of the current research. In this study, 12 tarantulas of the Nhandu chromatus species purchased as immature siblings and kept under standardised conditions for 2.5 years were anaesthetised with isoflurane and had 0.2 ml of haemolymph sampled and analysed for: total protein, glucose, calcium, phosphorous and uric acid. As well as having kept a diary of their daily feed intake and recorded dates of ecdysis, the exposure time to anaesthetic and perceived effectiveness were recorded. The variables originally proposed for this investigation are based on 2 separate feeding regimes, differing in terms of feed quantity and the effect on the aforementioned biochemical parameters. Upon receipt of the biochemical results from the first sampling, it became apparent that unexpected correlations could be made between the stage of ecdysis, susceptibility to anaesthesia and the total protein levels in the haemolymph. Those that were due to shed imminently, indicated by cessation in feeding, had recognisably and significantly higher total protein levels and reached a better level of anaesthesia in less time. Additional samplings are planned in the future to specify more definitive parameters. The observations made inadvertently so far could constitute novel information and be practically useful to tarantula enthusiasts and anaesthetists, and therefore, potentially of high clinical significance.
Ismail Mohamed Helal
Foeniculum vulgare and Pimpinella anisum L. Agronomie 9(3), 277-279. S ingh H.P., K aur S., B atish D.R., K ohli R.K., 2014. Ferulic acid impairs rhizogenesis and root growth, and alters associated biochemical changes in mung bean ( Vigna radiata ) hypocotyls. J. Plant Interact. 9, 267-274. S mith J.H.C., B enitez A., 1955. Chlorophylls analysis in plant materials. In: Modern Methods of Plant Analysis. vol. 4. K. Peach and M.V. Tracey (Eds), Springer-Verlag, Berlin, Germany, 142-196. S olecka D., K acperska A., 2003. Phenylpropanoid deficiency
Mohd. Rashdan Abd. Rahim, Jin Ai Mary Anne Tan, Ravishankar Ram Mani and Umah Rani Kuppusamy
Background: Beta-thalassaemia (β-thalassaemia) major patients are severely anaemic and require life-long blood transfusions for survival. These patients require iron-chelation therapy as a result of iron overload due to the monthly blood transfusions. The iron over load can cause oxidative damage and pro-inflammation and therefore, hasten mortality. Thus, regular monitoring of the oxidative stress and pro-inflammation status may be useful in these patients.
Methods: Measurement of biomarkers is usually performed on serum samples but the evaluation in non-invasive samples such as saliva would be more favourable in paediatric cases. In this study, the levels of pro-inflammatory cytokines such as tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) as well as oxidative indices such as lipid hydro peroxide, advanced oxidation protein products (AOPP), ferric reducing antioxidant power (FRAP), uric acid (UA) and glutathione peroxidase (GPx) activity in a total of 65 β-thalassaemia major patients (all on iron chelation) and 55 healthy control subjects were assessed. All the above biochemical parameters, measured using well established assay techniques, were detectable in saliva samples.
Results: Non parametric analyses showed that lipid hydroperoxide (LH) and glutathione peroxidase (GPx) activities were significantly higher in β-thalassaemia major patients. All other parameters were not significantly different between patient and control groups implying that iron chelation therapy was successful in attenuating oxidative stress. Strong positive correlation was observed between FRAP and UA levels. There was also a notable difference in tumour necrosis factor-α (TNF-α) between the patients and healthy controls when analysed according to ethnicity and age. AOPP level in β+-thalassaemia homozygous patients were significantly higher than β+/β0-compound heterozygous and β0-thalassaemia homozygous patients.
Conclusion: Saliva may serve as a reliable, non-invasive sample which can be used to assess oxidative indices and pro-inflammatory cytokines in β-thalassaemia major patients.
Agnieszka Noszczyk-Nowak, Marcin Michałek, Adrian Janiszewski, Agnieszka Kurosad, Agnieszka Sławuta, Alicja Cepiel and Urszula Pasławska
Electrical cardioversion is a therapeutic procedure used to convert various types of arrhythmias back to sinus rhythm. It is used to restore the sinus rhythm in dogs with atrial fibrillation. The effect of the electrical energy used during cardioversion on red blood cells (RBC) is not fully understood. Studies on humans reported lysis of RBC following electrical cardioversion. Similar studies have not been carried out on dogs. The aim of the study was to assess the effect of electrical cardioversion on chosen RBC parameters.
Material and Methods
The study was carried out on 14 large and giant breed dogs weighing from 30 to 84 kg with lone atrial fibrillation (lone AF). Electrical cardioversion was carried out under general anaesthesia by biphasic shock with 70–360 J of energy. Blood was collected at T0 – during atrial fibrillation, prior to cardioversion, and at T1 – 30 min after electrical cardioversion. Complete blood counts as well as total and direct bilirubin concentrations were evaluated. A maximum output of 360 J was used.
In all cases, electrical cardioversion was effective, and no significant changes in the number of RBC and RBC indices were noted. Similarly, there were no statistically significant differences in the levels of total and direct bilirubin.
Electrical cardioversion in dogs led neither to statistically nor clinically significant RBC lysis.
Petar Canović, Aleksandra Vranic, Sara Petrovic, Ivana Rakovic, Biljana Popovska Jovicic and Nedim Hamzagic
Primary infection with Epstein-Barr virus (EBV) usually occurs in early childhood and often does not present clinical symptoms. More than 90% of adults are infected with this virus. A primary infection that occurs in adolescence or adulthood is usually clinically presented as infectious mononucleosis with a triad of symptoms: fever, lymphadenopathy and pharyngitis. Our retrospective study included 51 patients with a median age of 17 (9-23) years and serologically confirmed infectious mononucleosis. All patients with infectious mononucleosis were treated at the Clinic for Infectious Diseases at the Clinical Center in Kragujevac during 2013. We analysed the clinical, haematological and laboratory parameters of patients. The aspartate-aminotransferase levels were increased in 40 patients, with a mean value of 116.24 (±93.22); the alanine-aminotransferase levels were increased in 44 patients, with a mean value of 189.24 (±196.69). Lymphadenopathy was the most common clinical feature upon admission in 49 patients (96%); 38 patients (74.5%) had splenomegaly, and 20 (39%) had hepatomegaly. Twenty-six patients (51%) had leukocytosis with lymphocytosis, while 15 (75%) of the 20 who had a normal leukocyte count also had lymphocytosis. In the present study, we updated the clinical, haematological and laboratory parameters, which may lead to the establishment of an accurate diagnosis and promote further treatment of the patients.
Gurgas Leonard, Rosoiu Natalia, Chirila Sergiu and Hangan Tony
://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC2890380 7. Leonard G, Nelu D-P, Sergiu C, Tony H, Natalia R, Olimpia M. Electron Microscopy Study of Nodular Basal Cell Carcinoma. ARS Medica Tomitana. 2018;24(2):90–5. 8. Gurgas L, Hangan T, Chirila S, Rosoiu N. Analysis methods of treatment as recurrent factor of basal cell carcinomas analysis methods of treatment as recurrent factor of basal cell carcinomas. Arch Balk Med Union [Internet]. 2016;51(3):347–51. Available from: http://umbalk.org/wp-content/uploads/2016/12/2016-3-347.pdf 9. Gurgaş L, Hangan T, Chirilă S, Roşoiu N. Environment and gender
Islam M. Sadiqul, Saimon Mohiful Kabir, Zannatul Ferdous, Khan Mst. Mansura and Rahman Md. Khalilur
recommended nutrient intakes. J Food Comp Ana 42 : 120–133. Bolognesi C, Hayashi M. (2011). Micronucleus assay in aquatic animals. Muta-genesis 26 : 205–213. Cadet J, Douki T, Gasparutto D, Ravanat JL. (2003). Oxidative damage to DNA: formation, measurement and biochemical features. Mut Res 531 : 5–23. Carrasco KR, Tilbury KL, Meyers MS. (1990). Assessment of the piscine micro-nucleus test as an in situ biological indicator of chemical contaminant effects. Can J Fish Aquat Sci 47 : 2123–2136. Chauhan LKS, Chandra S, Saxena PN, Gupta SK