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Tomasz Śniegocki, Marta Giergiel, Bartosz Sell and Andrzej Posyniak

involves the solid-phase extraction (SPE) approach for confirmation and quantitation of residues of these compounds in biological matrices using ultra-high-pressure liquid chromatography-MS/MS (UHPLC–MS/MS). The aim of this research is elaboration of the first method for determination of nitrofurans and nitrofuran metabolites using UHPLC-MS/MS in a single analytical procedure. Material and Methods Materials All reagents were of minimum analytical grade or higher. Materials were procured as previously described ( 13 ) or as follows: ethyl acetate, hexane (95

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Małgorzata Gbylik-Sikorska, Anna Gajda, Artur Burmańczuk, Tomasz Grabowski and Andrzej Posyniak

References 1. de Boer V.C., Dihal A.A., van der Woude H., Arts I.C., Wolffram S., Alink G.M., Rietjens I.M., Keijer J., Hollman P.C.: Tissue distribution of quercetin in rats and pigs. J Nutrit 2005, 135, 1718–1725. 2. Chang L., Ren Y., Cao L., Sun Y., Sun Q., Sheng N., Yuan L., Zhi X., Zhang L.: Simultaneous determination and pharmacokinetic study of six flavonoids from Fructus Sophorae extract in rat plasma by LC–MS/MS. J Chrom B 2012, 904, 59–64. 3. Coppin J.P., Xua Y., Chena H., Pan M.H., Hoc Ch.T., Juliani R., Simon J.E., Wu Q

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Ingars Reinholds, Iveta Pugajeva and Vadims Bartkevičs

”, Microchemical Journal, 2013, vol. 107, pp. 165-171. http://dx.doi.org/10.1016/j.microc.2012.05.035 [10] X. Liang, B. Chen, X. Nie, Z. Shi, X., Li, X. Huang, “The Distribution and Partitioning of Common Antibiotics in Water and Sediment of the Pearl River Estuary, South China”, Chemosphere, 2013, vol. 92, N11, pp. 1410-1416. http://dx.doi.org/10.1016/j.chemosphere.2013.03.044 [11] J. Rossmann, S. Schubert, R. Gurke, R. Oertel, W. Kirch, “Simultaneous Determination of Most Prescribed Antibiotics in Multiple Urban Wastewater by SPE-LC-MS/MS

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Robert Skibinski and Lukasz Komsta

Abstract

Ultra high performance liquid chromatography (UHPLC), coupled with accurate quadrupole-time-of-flight (Q-TOF) mass spectrometry, was used for the stability study of acetylsalicylic acid within a variety of different organic solutions: methanol, ethanol, propan-2-ol, acetonitrile, tetrahydrofuran and 1,4-dioxane. With the use of gradient elution chromatography and mass spectrometry detection in negative ionization, MS and MS/MS spectra were recorded simultaneously. In addition, quantitative, as well as qualitative analysis was performed during one assay. The stability of acetylsalicylic acid in such solutions was tested at room temperature, in a 12h period. In the work, in all cases, only one main degradation product, salicylic acid, was found. What is more, the work revealed that the degradation of aspirin in the tested organic solutions yields apparent second-order kinetics. The study also demonstrated that acetonitrile and 1,4-dioxane turned out to be the most stable solvents, and an above 80% of initial concentration of acetylsalicylic acid was found in this case. Furthermore, the most popular analytical solvents, methanol and ethanol, were found to be very unstable media. Herein, below 40% of initial concentration of acetylsalicylic acid was seen after 12h. The obtained results were also compared with the degradation of acetylsalicylic acid in a water solution. In this situation, only about 25% of the analyzed compound was resolved to salicylic acid in the same time frame.

Open access

Małgorzata Kikowska, Barbara Thiem, Elwira Sliwinska, Monika Rewers, Mariusz Kowalczyk, Anna Stochmal and Jolanta Długaszewska

. Determination of polyphenols in Mentha longifolia and M. piperita field-grown and in vitro plant samples using UPLC-TQ-MS. Journal of AOAC International 94: 43–50. K usakari K, Y okoyama M, and I nomata S. 2000. Enhanced production of saikosaponins by root culture of Bupleurum falcatum L. using two-step control of sugar concentration. Plant Cell Reports 19(11): 1115–1120. K ÿpeli E, K artal M, A slan S, and Y esilada E. 2006. Comparative evaluation of the anti-inflammatory and antinociceptive activity of Turkish Eryngium species. Journal of

Open access

Małgorzata Kikowska, Agata Włodarczyk, Anna Stochmal, Jerzy Żuchowski and Barbara Thiem

Summary

Introduction: Callus and cell suspension cultures are widely applied in investigation of production of high-value secondary metabolites, which may be used as cosmeceuticals, nutraceuticals and pharmaceuticals. Plant cell cultures are promising alternative to intact plant sources for the production of plant-derived drugs of industrial importance.

Objective: The aim of the study was to (i) initiate the cell suspension culture of Chaenomeles japonica from homogenous and uniform callus, (ii) stabilize the selected line and (iii) verify its ability to produce the desired groups of secondary metabolites – pentacyclic triterpenoids and polyphenols.

Methods: To establish a cell suspension culture, stabilized and homogeneous callus was selected. Cell cultures were systematically passaged every 2 weeks to fresh liquid medium with the same composition. Biomass from cultures at the growth phase and stationary phase was designated for phytochemical research. UHPLC-DAD-MS analyzes were performed. At the same time, their macroscopic and microscopic observations were carried out.

Results: Cells of suspension culture line A2 were characterized by the intense divisions. Cell culture extracts (both from the growth phase and stationary phase) contained pentacyclic triterpenoids. In addition, phe-nolic compounds (chlorogenic acid and proanthocyanidins type B) and in a small amount also epicatechin are present in the extract of the cells harvested from the growth phase. In the present studies, three pentacyclic triterpenoids were detected and quantified in the extracts of cell suspensions and callus line A2. Ursolic and oleanolic acids were the main triterpenoids in the studied extracts. The cell suspension culture from the growth phase exhibited the highest content of ursolic, oleanolic, and betulinic acid (separately and together).

Conclusion: The cell suspension culture of Chaenomeles japonica is a promising source of pentacyclic triterpenoids.

Open access

Gorica Vuković, Vojislava Bursić, Tijana Stojanović, Aleksandra Petrović, Sonja Gvozdenac, Mira Starović, Slobodan Kuzmanović and Goran Aleksić

tropane alkaloids present in organic foods? Detection of scopolamine and atropine in organic buckwheat (Fagopyron esculentum L.) products by UHPLCMS/MS, Food Chemistry 239, 141–147, 2018. SANTE/11813/2017: Method validation and quality control procedures for pesticide residues analysis in food and feed. ZHENG, W., YOO, K.H., MINCHOI, J., PARK, D., KIM, S., KANG, Y., ABD EL-ATY, A.M., HACIMÜFTÜOĞLU, A., JEONG, J.H., EL-DIN BEKHIT, A., SHIM, J., SHIN, H.: A modified QuEChERS method coupled with liquid chromatography-tandem mass spectrometry for the

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Kamila Mitrowska, Maja Antczak and Andrzej Posyniak

- performance liquid chromatography coupled to time of flight mass spectrometry. J Chromatogr B 2009, 877, 2363-2374. 11. Robert C., Gillard N., Brasseur P.Y., Pierret G., Ralet N., Dubois M., Delahaut P.: Rapid multi-residue and multi-class qualitative screening for veterinary drugs in foods of animal origin by UHPLC-MS/MS. Food Add Contamin Part A - Chem Anal Control Exposure Risk Assess 2013, 30, 443-457. 12. Stolker A.A., Rutgers P., Oosterink E., Lasaroms J.J., Peters R.J., van Rhijn J.A., Nielen M.W.: Comprehensive screening and

Open access

Marie Mardal, Frank Møller Aarestrup, Brian Schou Rasmussen, Christian Brinch Mollerup, Petur Weihe Dalsgaard and Kristian Linnet

] Bade R, Rousis NI, Bijlsma L, Gracia-Lor E, Castiglioni S, Sancho JV et al. Screening of pharmaceuticals and illicit drugs in wastewater and surface waters of Spain and Italy by high resolution mass spectrometry using UHPLC-QTOF MS and LC-LTQ-Orbitrap MS. Anal Bioanal Chem 2015; 407(30):8979-8988. [23] Bijlsma L, Emke E, Hernandez F, de VP. Investigation of drugs of abuse and relevant metabolites in Dutch sewage water by liquid chromatography coupled to high resolution mass spectrometry. Chemosphere 2012; 89(11):1399-1406. [24] Hermanson GT. Bioconjugate

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Katarína Hroboňová, Jozef Lehotay and Jozef Čižmárik

., SOLICH, P.: Development and application of UHPLC - MS/MS method for the determination of phenolic compounds in Chamomile flowers and Chamomile tea extracts. Talanta, 82, 2010, 1271 - 1280. SFORCIN, J.M., BANKOVA, V.: Propolis: is there a potential for the development of new drugs. Rewiev. J. Ethnopharmacology, 133, 2011, 253-260. SILICI, S., KUTLUCA, S.: Chemical composition and antibacterial activity of propolis collected by three different races of honeybees in the same region. J. Ethnopharmacol., 99, 2005, 69-73. SU, J