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Joanna Pławińska-Czarnak, Joanna Zarzyńska, Janusz Bogdan, Alicja Majewska, Marek Karwański, Magdalena Kizerwetter-Świda, Jarosław Kaba, Krzysztof Anusz and Emilia Bagnicka

. Microbiol. 54: 291-331. Dalaly B.K., Eitenmiller R.R., Friend B.A., Shahani K.M. (1980). Human milk ribonuclease. Biochim. Biophys. Acta 65(2): 381–391. Dastgheib S., Irajie C., Assaei R., Koohpeima F., Mokarram P. (2014). Optimization of RNA extraction from rat pancreatic tissue. Iran. J. Med. Sci. 39(3): 282-288. Dudemaine P.L., Fomenky B., Dutoit A., Béjanin L., Ibeagha-Awemu E.M. (2017). 341 Comparison of five commercial kits for total RNA isolation including microRNA from three bovine milk fractions. J. Anim. Sci. 95(4): 168–169. Finot L

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Pwaveno Huladeino Bamaiyi, Mohd Sani Nani Izreen, Khatijah Mohamad, Badrul Hisham Nur Eizzati, Che Rostman Siti-Bainum, Mat Kamir Norsyamimi Farhana, Mohd Nor Mohd Norfaizull, Saeid Kadkhodaei and Goriman Khan Mohd Azam Khan

during the course of a devastating flood, may have affected some parameters that could have improved this study. The use of only culturing of water samples and looking for only bacterial colonies that grew within 48 hours may have excluded other bacteria such as Leptospira spp, Vibrio spp, and Burkholderiapseudomallei from being documented. Nevertheless, the isolation of several species of bacteria and genera of bacteria indicates a rich bacterial biodiversity of pathogenic or potentially pathogenic bacteria in the flood water during the great flood of December

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Aleš Eichmeier, Eliška Peňázová, Jakub Pečenka, Jana Čechová, Robert Pokluda, Dorota Tekielska and Miroslav Baránek

microbiological storage stability of minimally processed fruits and vegetables. Food Technology 43 (2): 132-139. Klindworth A., Pruesse E., Schweer T., Peplies J., Quast C., Horn M., Glöckner F.O. 2013. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies. Nucleic Acids Research. Available on: https://doi.org/10.1093/nar/gks808 [Accessed: September 4, 2016] Liao C.H., Wells J.M. 1987. Association of pectolytic strains of Xanthomonas campestris with soft rots of fruits and

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C. M. Patreze, D. B. Felix, F. R. Scarano and M. Alves-Ferreira

and P. C. SHARMA (2009): Isolation of good quality RNA from a medicinal plant seabuckthorn, rich in secondary metabolites. Plant Physiology and Biochemistry 47: 1113-1115. DOI: 10.1016/j.plaphy.2009.09.004. GONZÁLEZ-MARTÍNEZ, S. C., K.V. KRUTOVSKY and D. B. NEALE (2006): Forest-tree population genomics and adaptive evolution. New Phytologist 170 (2): 227-238. DOI: 10.1111/j.1469-8137.2006.01686.x. HOLL, K. D. (1998): Effects of above- and below-ground competition of shrubs and grass on Calophyllum brasiliense (Camb.) seedling growth

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Hanna Czekaj, Wojciech Kozdruń, Natalia Styś-Fijoł, Jowita Samanta Niczyporuk and Karolina Piekarska

Introduction For many years in the poultry population in Poland, as in many other countries, there has been a significant spread of infections with avian reovirus (ARV) ( 4 ). The virus belongs to the genus Orthoreovirus , family Reoviridae ( 14 ). Its genome is double-stranded, segmented RNA (dsRNA) consisting of 10–12 segments coding for structural and non-structural viral proteins. The genetic material is surrounded by a capsid with twenty-sided symmetry consisting of eight structural polypeptides. The genome structure can be a source of variation

Open access

Filiz Kizilates, Hande Berk, Melahat Coban, Derya Seyman, Metin Sarikaya, Funda Sari and Nefise Oztoprak

erythropoietin to treat anemia and to reduce the need of transfusion, the widespread use of new generation diagnostic assays, and isolation of HBsAg seropositive patients has led to a decrease in the prevalence of HBV seropositivity among patients who undergo HD. Nevertheless, prevalence remains high compared to normal populations [ 1 , 8 , 11 , 12 ]. Isolation of HBsAg seropositive patients and the equipment used for patients with susceptibility for HBV may decrease prevalence of HBV infection by up to 70%–80% [ 8 , 12 ]. Vaccination programs and limitation of blood

Open access

H Oghbaei, N Ahmadi Asl and F Sheikhzadeh

factor (NF)-κB signaling pathway in rat skeletal muscle. FASEB J 18, 1499–1506, 2004. Lasser C, Eldh M, Lotvall J. Isolation and characterization of RNA-containing exosomes. JoVE 59, e3037, 2012. Luis-Rodriguez D, Martinez-Castelao A, Gorriz JL, De-Alvaro F, Navarro-Gonzalez JF. Pathophysiological role and therapeutic implications of inflammation in diabetic nephropathy. World J Diabetes 3, 7–18, 2012. Ma Z, Qi J, Meng S, Wen B, Zhang J. Swimming exercise training-induced left ventricular hypertrophy involves microRNAs and synergistic regulation of

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Marcin Feldo, Janusz Kocki, Jan Feldo, Sylwia Łukasik, Jacek Bogucki, Adam Skwarzyński, Jacek Wroński, Jan Kęsik and Tomasz Zubilewicz

, Jayarajasingam R, Cottam R et al.: Cocharane Database Syst Rev. 2014; 23: 1: CD004178.doi:10.1002/14651858. 13. Chomczyński P, Sacchi N: Single-step metod of RNA isolation by acid guanidinum thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162: 156-59. 14. Li D, Da L, Tang H et al.: CpG methylation plays a vital role in determining tissue- and cell-specific expression of the human cell-death-inducing DFF45-like effector A gene through the regulation of Sp1/Sp3 binding. nucleic Acids res 2008; 36: 330-41. 15. SiemińskaL: Tkanka tłuszczowa

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Taja Lozar, Klara Gersak, Maja Cemazar, Cvetka Grasic Kuhar and Tanja Jesenko

), cell free RNA (cfRNA), microRNA (miRNA) and exosomes. Detection of CTCs in a blood sample is challenging as these cells are present in very small numbers and are surrounded by billions of other blood cells (1 CTC per 10 7 of leukocytes per ml of blood). 78 Highly sensitive and specific analytical methods are required, which can be achieved by using capture, enrichment and detection procedures. 79 Most isolation devices combine capture and enrichment procedures and may also include detection and enumeration technology. Capture procedures aim to overcome the low

Open access

I. SvecovA, E. Jezkova, I Hudecova, T. Burjanivova, K. Biskupska-Bodova, J. Danko and Z. Lasabová

Non-Invasive Fetal Sex Determination Using SRY Specific Primers and Sybrgreen Real Time PCR

Presence of fetal cells and circulating free fetal DNA and RNA in maternal circulation represents the basic concept in developement of non-invasive prenatal diagnostic methods based on molecular biology and genetics. We introduced new methods for free fetal DNA isolation and detection in maternal circulation via DNA isolation from maternal plasma using real-time PCR SYBRGreen targeting and newly designed primers focused in SRY sequence. We determined gender in 46 singleton pregnancies, 22 boys and 24 girls and assessed the analytical and clinical validity. We reached 95.45% sensitivity and 95.83% specificity. We suggest improvements in molecular-biological procedures in the discussion, which could be used in studies of clinical utility of non-invasive prenatal diagnosis (NIPD) in decreasing amount of invasive procedures unnecessarily performed.