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Marcin Ożarowski, Radosław Kujawski, Przemysław Mikołajczak, Agnieszka Gryszczyńska, Aurelia Pietrowiak, Wojciech Białas, Justyna Baraniak, Małgorzata Górska-Paukszta, Waldemar Buchwald, Bogdan Kędzia, Anna Krajewska-Patan and Agnieszka Seremak-Mrozikiewicz

. European Pharmacopoeia. Monograph: greater celandine ( Chelidonii herba ). Sixth Edition, EDQM, Council of Europe, Strasbourg, 2011:1145-1146. 22. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebenson Wiss Technol 1995; 28:25-30. 23. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999; 26(9/10):1231-1237. 24. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP

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Paulina Malinowska

therapeutic uses of Sea Buckthorn (Hippophae): a review. J Biol Sci 2004; 4 (5):687-693. 43. Karadeniz F, Burdurlu HS, Koca N, Soyer Y. Antioxidant activity of selected fruits and vegetables grown in Turkey. Turk J Agric For 2005; 29 (4):297-303. 44. Benzie IFF, Strain JJ. The Ferric Reducing Ability of Plasma (FRAP) as a Measure of Antioxidant Power: The FRAP Assay. Anal Biochem 1996; 239:70-76 45. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying improved ABTS radical cation

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Marta Pudzianowska, Marek Gajewski, Jarosław L. Przybył, Agnieszka Buraczyńska, Olga Gaczkowska, Marta Matuszczak and Marta Dziechciarska

References Bartoń H., Fołta M., Zachwieja Z. 2005. [Application of FRAP, ABTS and DPPH methods to estimation of antioxidant activity of food products.] Nowiny Lekarskie 74(4): 510-513. [in Polish with English summary] Bartz J.A, Brecht J.K. (ed.). 2003. Postharvest physiology and pathology of vegetables. Marcel Dekker Inc., New York. Beesk N., Perner H., Schwarz D., George E., Kroh L. W., Rohn S. 2010. Distribution of quercetin-3,4-Odiglucoside, quercetin-4-O-monoglucoside, and quercetin in different

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Anna M. Witkowska and Małgorzata E. Zujko

References 1. AHA Scientific Statement: Diet and Lifestyle Recommendations Revision 2006. A Scientific Statement from the American Heart Association Nutrition Committee. Circulation, 2006, 114, 82-96. 2. Alavanja M.C.R., Brown C.C., Swanson C., Brownson R.C., Saturated fat intake and lung cancer risk among nonsmoking women in Missouri. J. Natl. Cancer Inst., 1993, 85, 1906-1916. 3. Benzie I.F.F., Strain J.J., The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay

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Aneta Sławińska, Wojciech Radzki and Janusz Kalbarczyk

of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Viticult 1965; 16:144-158. 37. Shimada K, Fujikawa K, Yahara K, Nakamura T, Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agr Food Chem 1992; 40:945-948. 38. Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay. Anal Biochem 1996; 239:70-76. 39. Alvarez-Parrilla E, de la Ros LA, Torres-Rivas F, Rodrigo-Garcia J, Gonzalez

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Noémi Koczka, Zsuzsanna Móczár, Éva Stefanovits-Bányai and Attila Ombódi

-158. 12. I. F. F. Benzie and J. J. Strain, Ferric reducing ability of plasma (FRAP) as a measure of antioxidant power: The FRAP assay, Anal. Biochem. 239 (1996) 70-76; DOI: 10.1006/abio.1996.0292. 13. J. Kobus, E. Flaczyk, A. Siger, M. Nogala-Kalucka, J. Korczak and R. B. Pegg, Phenolic compounds and antioxidant activity of extracts of Ginkgo leaves, Eur. J. Lipid Sci. Technol. 111 (2009) 1150-1160; DOI: 10.1002/ejlt.200800299. 14. H. Aoshima, S. Hirata and S. Ayabe, Antioxidative and anti-hydrogen peroxide activities of various herbal

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Hakime Hülya Orak, Magdalena Karamać, Adnan Orak and Ryszard Amarowicz

.G. Arntfield S.D. Physical and nutritional impact of fortification of corn starch-based extruded snacks with common bean ( Phaseolus vulgaris L.) flour: Effects of bean addition and extrusion cooking Food Chem. 2009 113 989 996 8. Benzie I.F.F., Strain J.J., The Ferric Reducing Ability of Plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem., 1996, 239, 70–76. Benzie I.F.F. Strain J.J. The Ferric Reducing Ability of Plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay Anal Biochem. 1996 239

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Silvia Mošovská, Patrícia Petáková, Michal Kaliňák and Anna Mikulajová


The evaluation of antioxidant potential of food has received much attention in recent years. Antioxidant compounds can scavenge free radicals and thereby can protect the human body from free radicals. This study was focused on the isolation of curcuminoids from the dried turmeric rhizome, and studying their antioxidant activity. The presence of curcuminoids was identified in turmeric sample by Nuclear Magnetic Resonance (NMR) analysis. Since neutral curcumin is known to be poorly soluble, the synthesis of curcumin-cyclodextrin and curcumin-phospholipid complexes was also performed. The antioxidant activity of isolated curcuminoids was assessed by two methods (ABTS and FRAP assay) and their scavenging activities were compared with those of prepared complexes. The ability to reduce ABTS radical cation decreased as follows: quercetin > trolox > curcuminoids > curcumin-cyclodextrin complex > curcumin-phospholipid complex. The reducing potential of tested samples in descending order was quercetin > trolox > curcumin-cyclodextrin complex > curcuminoids > curcumin-phospholipid complex.

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Anna Muzykiewicz, Joanna Zielonka-Brzezicka and Adam Klimowicz


Introduction: Quince (Cydonia oblonga Mill.) is a plant of which both the fruits and the leaves are sources of compounds with antioxidant potential. Such activity could be helpful to prevent the development of so-called oxidative stress.

Objective: The aim of the study was to evaluate the antioxidant properties of ethanolic, methanolic and acetonic extracts of mature and immature quince fruits, as well as leaves.

Methods: The extracts were prepared using ultrasound-assisted extraction, for 15, 30 and 60 minutes. The antioxidant activity was assessed by DPPH, FRAP, ABTS and Folin-Ciocalteu (F-C) methods.

Results: Antioxidant activity of all of the evaluated extracts were observed. The highest potential determined with each method was found for leaf extracts. Moreover, higher activity of unripe fruit extracts compared to ripe fruit was observed. Taking into account the applied extractants, the highest antioxidant capacity was found for methanolic extracts, extracted for 60 and 30 minutes. By contrast, the lowest potential was observed mainly for ethanolic extracts (extraction time 15 minutes).

Conclusion: Quince extracts, particularly alcoholic extracts of leaves, seem to be a valuable source of anti-oxidants. Factors as extraction time, the type of solvent and degree of fruit maturity may influence the antioxidant activity of extracts.

Open access

Dominika Andrys, Danuta Kulpa, Monika Grzeszczuk and Bożena Białecka

Abbreviations ABTS – determination of free radical-scavenging ability by the use of a stable 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical cation DPPH – determination of 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity FRAP – determination of ferric reducing antioxidant power GAE – gallic acid JA – jasmonic acid LSD – least significant difference MS – Murashige and Skoog medium TAA – total antioxidant activity TE – trolox equivalent, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid REFERENCES A