Katarzyna Rafinska, Krzysztof Zienkiewicz and Elzbieta Bednarska
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Tomáš Štrba, Ingrid Turisová and Štefan Aschenbrenner
The paper presents the results of floristic and phytosociological research conducted during growing season 2011 on a copper mine heap Richtárová, near the village Staré Hory situated in buffer zone of the Low Tatras National Park. In total, we found 147 taxa of vascular plants. The dominant plant species of the mine heap is Agrostis capillaris, which sometimes forms relatively continuous cover, and other species growing in small islands of plants tolerant to specific environmental conditions, especially Silene dioica, Acetosella vulgaris, Arabidopsis arenosa. We sampled 8 phytosociological relevés. Localities of relevés were selected considering variability of plant communities on the all mine heap.
Anna Grobelak, Anna Napora and Małgorzata Kacprzak
The main purpose of this study was to evaluate impact of plant growth promoting bacteria (PGPB) on the development of phytopathogenic fungi and correlate it with a potential effects on the growth of plants under unfavorable conditions, in order to improve the efficiency of a phytoremediation process. The conducted research focused on the antifungal properties of PGPB. In this study, 51 isolates of bacteria were obtained after diversified disinfection time from plants growing on soil after sewage sludge amendment. The results revealed that some isolated bacteria, mainly endophytic ones, inhibited the development of Fusarium oxysporum, F. culmorum and Alternaria alternata.
A Global Microarray Expression Profile of Grapevine miRNAs Isolated from Cell Suspensions Pre-Treated with Apoptosis Activators
In an attempt to identify novel and apoptosis/pathogen-regulated microRNAs (miRNAs) and small interfering RNAs, we performed a robust microarray screening of small RNA population from Vitis vinifera L. cv. Limberger cell suspension exposed to apoptosis activators (e.g. methyl jasmonate) or elicitors (botrycin and cinerein) derived from necrotrophic fungus Botrytis cinerea Pers. et Fries. Using a microarray expression profiling approach, we identified 22 miRNAs. We found that a majority of these miRNAs were predicted to target stress/defense-related genes of plants. Of the 22 V. vinifera miRNAs, 11 have sequence conservation in Arabidopsis thaliana but exhibited species-specific developmental and/or stress/defense-related expression patterns. Ten of the miRNAs are highly conserved in other plant species, suggesting that even conserved miRNAs may have different regulatory roles in various species. Our results show that these grapevine miRNAs can be also induced by various apoptosis inducers. Fifty-one potential targets were predicted to the newly identified miRNAs based on sequence complementarity. In addition to miRNAs, we identified 102 other novel endogenous small RNAs in Vitis, indicating that a large number of miRNAs and other small regulatory RNAs are encoded by the Vitis vinifera genome.
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