-312. Markham KR, and Geiger H. 1994. 1H Nuclear magnetic resonance spectroscopy of flavonoids and their glycosides in hexadeuterodimethylsulfoxide. In: Harborne JB [ed.], The Flavonoids Advances in Research since 1986, 441-497. Chapman and Hall, London, UK. Miller NJ, and Rice-Evans CA. 1996. Spectrophotometric determination of antioxidant activity. Redox Report 2: 161-171. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, and Rice-Evans C. 1999. Antioxidant activity applying an improved ABTS radical cation
Krzysztof Pawlak, Wieslawa Bylka, Beata Jazurek, Irena Matlawska, Maria Sikorska, Henryk Manikowski and Grazyna Bialek-Bylka
Marcin Ożarowski, Radosław Kujawski, Przemysław Mikołajczak, Agnieszka Gryszczyńska, Aurelia Pietrowiak, Wojciech Białas, Justyna Baraniak, Małgorzata Górska-Paukszta, Waldemar Buchwald, Bogdan Kędzia, Anna Krajewska-Patan and Agnieszka Seremak-Mrozikiewicz
. European Pharmacopoeia. Monograph: greater celandine ( Chelidonii herba ). Sixth Edition, EDQM, Council of Europe, Strasbourg, 2011:1145-1146. 22. Brand-Williams W, Cuvelier ME, Berset C. Use of free radical method to evaluate antioxidant activity. Lebenson Wiss Technol 1995; 28:25-30. 23. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999; 26(9/10):1231-1237. 24. Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP
Martin Mellen, Martina Fikselová, Andrea Mendelová and Peter Haščík
honeys, before and after in-vitro digestion. Pol. J. Food Nutr. Sci., 2013, 63, 167-171. 17. Re R., Pellegrini N., Protegente A., Pannala M. et al., Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Biol. Med., 1999, 26, 1231-1237. 18. Saxena S., Gautam S., Sharma A., Physical, biochemical and antioxidant properties of some Indian honeys. Food Chem., 2010, 118, 391-397. 19. Silva T.M.S., Santos F.P, Evangelista-Rodrigues A. et al., Phenolic compounds, melissopalynological
Hakime Hülya Orak, Magdalena Karamać, Adnan Orak and Ryszard Amarowicz
161 254 260 29. Re R., Pellegrini N., Proteggente A., Pannala A., Yang M., Rice-Evans C., Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Rad. Biol. Med., 1999, 26, 1231–1237. Re R. Pellegrini N. Proteggente A. Pannala A. Yang M. Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay Free Rad. Biol. Med. 1999 26 1231 1237 30. Ross K.A., Beta T., Arntfield S.D., A comparative study on the phenolic acids identified and quantified in dry
Amira Benahmed, Boualem Harfi, Ibtissem Benbelkacem, Abla Daas, Hocine Laouer and Abdelmalik Belkhiri
Wiss Technol . 1995;28:25-30. 9. Barkat M, Laib I. Chemical composition and antioxidant activity of the essential oil of dry flowers of Lavandula officinalis . Ind Eng J. 2011;6:46-54. 10. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Bio Med . 1999;26:1231-7. 11. Apak R, Güçlü K, Özyürek M, Karademir SE. Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability
Dominika Andrys, Danuta Kulpa, Monika Grzeszczuk, Magdalena Bihun and Agnieszka Dobrowolska
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Silvia Mošovská, Patrícia Petáková, Michal Kaliňák and Anna Mikulajová
The evaluation of antioxidant potential of food has received much attention in recent years. Antioxidant compounds can scavenge free radicals and thereby can protect the human body from free radicals. This study was focused on the isolation of curcuminoids from the dried turmeric rhizome, and studying their antioxidant activity. The presence of curcuminoids was identified in turmeric sample by Nuclear Magnetic Resonance (NMR) analysis. Since neutral curcumin is known to be poorly soluble, the synthesis of curcumin-cyclodextrin and curcumin-phospholipid complexes was also performed. The antioxidant activity of isolated curcuminoids was assessed by two methods (ABTS and FRAP assay) and their scavenging activities were compared with those of prepared complexes. The ability to reduce ABTS radical cation decreased as follows: quercetin > trolox > curcuminoids > curcumin-cyclodextrin complex > curcumin-phospholipid complex. The reducing potential of tested samples in descending order was quercetin > trolox > curcumin-cyclodextrin complex > curcuminoids > curcumin-phospholipid complex.
Anna Muzykiewicz, Joanna Zielonka-Brzezicka and Adam Klimowicz
Introduction: Quince (Cydonia oblonga Mill.) is a plant of which both the fruits and the leaves are sources of compounds with antioxidant potential. Such activity could be helpful to prevent the development of so-called oxidative stress.
Objective: The aim of the study was to evaluate the antioxidant properties of ethanolic, methanolic and acetonic extracts of mature and immature quince fruits, as well as leaves.
Methods: The extracts were prepared using ultrasound-assisted extraction, for 15, 30 and 60 minutes. The antioxidant activity was assessed by DPPH, FRAP, ABTS and Folin-Ciocalteu (F-C) methods.
Results: Antioxidant activity of all of the evaluated extracts were observed. The highest potential determined with each method was found for leaf extracts. Moreover, higher activity of unripe fruit extracts compared to ripe fruit was observed. Taking into account the applied extractants, the highest antioxidant capacity was found for methanolic extracts, extracted for 60 and 30 minutes. By contrast, the lowest potential was observed mainly for ethanolic extracts (extraction time 15 minutes).
Conclusion: Quince extracts, particularly alcoholic extracts of leaves, seem to be a valuable source of anti-oxidants. Factors as extraction time, the type of solvent and degree of fruit maturity may influence the antioxidant activity of extracts.
Dominika Andrys, Danuta Kulpa, Monika Grzeszczuk and Bożena Białecka
Abbreviations ABTS – determination of free radical-scavenging ability by the use of a stable 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid radical cation DPPH – determination of 2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity FRAP – determination of ferric reducing antioxidant power GAE – gallic acid JA – jasmonic acid LSD – least significant difference MS – Murashige and Skoog medium TAA – total antioxidant activity TE – trolox equivalent, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid REFERENCES A
Anna Muzykiewicz, Joanna Zielonka-Brzezicka and Adam Klimowicz
Introduction: Antioxidants, isolated from different plant parts, are widely used due to their ability to prevent the development of so-called oxidative stress. Sea buckthorn (Hippophae rhamnoides L.) is one of the plants with expected antioxidant properties.
Objective: The aim of the study was to evaluate the antioxidant activity of ethanolic, methanolic and acetonic extracts of H. rhamnoides leaves, ripe and unripe fruits obtained by ultrasound-assisted extraction.
Methods: To estimate the antioxidant potential of the extracts the DPPH, FRAP, ABTS and Folin-Ciocalteu methods were applied. Moreover, the influence of the extrahent, as well as extraction time, on this activity was evaluated.
Results: Sea buckthorn leaf extracts showed higher activity, contrary to the fruit extracts. Moreover, higher activity of ripe fruit extracts compared to unripe material extracts was found. To obtain the highest content of antioxidants in the extracts, ultrasound-assisted extraction for 60 min with methanol should be applied.
Conclusions: The presented in vitro results could lead to the conclusion that H. rhamnoides seems to be a valuable source of antioxidants to be applied in various branches of industry.