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Sodium chloride-induced conformational change in tRNA as measured by circular dichroism

particular importance of the maximal velocity , J. Biol. Chem. 245 , 2465. Ishida T. & Sueoka N.; (1968), Tryptophan transfer RNA as the UGA suppressor , J. Biol. Chem. 243 , 5329. Kawai G., Ue H., Yasuda M., Sakamato K., Hashizume T. McCloskey JA., Miyazawa T. & Yokoyama S. (1991), Nucleic Acids Symp. Ser. 25 , 49-50. Kay C. M. & Willick G. E.; (1971), Magnesium-induced conformational change in transfer ribonucleic acid as measured by circular dichroism , Biochemistry, 10(12) , 2216–2222. Kelly S. M. & Pric N. C.; (2000), The Use of Circular

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Electrochemical impedimetric detection of stroke biomarker NT-proBNP using disposable screen-printed gold electrodes

of Neurological Disorders and Stroke rt-PA Stroke Study Group. The New England journal of medicine. 1995 Dec 14;333(24):1581-7. PubMed PMID: 7477192. Epub 1995/12/14.eng. 6. Hacke W, Kaste M, Bluhmki E, Brozman M, Davalos A, Guidetti D, et al. Thrombolysis with alteplase 3 to 4.5 hours after acute ischemic stroke. The New England journal of medicine. 2008 Sep 25;359(13):1317-29. PubMed PMID: 18815396. Epub 2008/09/26.eng. 7. Sandercock P, Wardlaw JM, Lindley RI, Dennis M, Cohen G, Murray G, et al. The benefits and harms of

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Influence of OASL gene polymorphisms on host response to interferon therapy in chronic hepatitis C virus patients

, Antonelli G, Antonaci S. MxA and PKR expression in chronic hepatitis C. J Interferon Cytokine Res. 2004 Nov;24(11):659-63. PubMed PMID: 15684819. Epub2005/02/03. eng. 24. Chen L, Borozan I, Feld J, Sun J, Tannis LL, Coltescu C, et al. Hepatic gene expression discriminates responders and nonresponders in treatment of chronic hepatitis C viral infection. Gastroenterology. 2005 May;128(5):1437-44. PubMed PMID: 15887125. Epub 2005/05/12. eng. 25. Marie I, Rebouillat D, Hovanessian AG. The expression of both domains of the 69/71 kDa 2’,5

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Compared salt tolerance of five local wheat (Triticum aestivum L.) cultivars of Albania based on morphology, pigment synthesis and glutathione content

+ xanthophylls in mg/g fresh weight chla in mg/g fresh weight chlb in mg/g fresh weight tot chl carotenoids + xanthophylls in mg/g fresh weight Suba 0 mM 0.60±0.25 a 0.147±0.5 a 0.75±0.29 a 42.8±15.8 a 0.59±0.01 a 0.12±0.01 a 1.18±0.05 a 46.9±0.1 a 50 mM 0.78±0.27 a 0.21±0.08 a 0.98±0.35 a 55.4±15.9 a 1.097±0.15 b 0.17±0.05 b 0.74±0.03 b 58.22±0.56 b 100 mM 1.01±0.07 a 0.255±0.02 a 1.26±0.09 a 65.2±10.4 a 1.06±0.01 c 0.24±0.14 c 1.34±0.013 b 68.5±0.55 c 200 mM 0.77±0.26 a 0.21±0.07 a 0

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Evolution of phytochemical and antioxidant activity of Tunisian carob (Ceratonia siliqua L.) pods during maturation

SK KE MN TN ZG GB Fe (μ mol/g DW) Unripe 0,40±0,00 Cc 0,21±0,00 Bb 0,37±0,00 Cc 0,25±0,00 Bb 0,17±0,00 Ca 0,91±0,00 Cd Mid-ripe 0,15±0,00 Bb 0,14±0,00 Ab 0,21±0,00 Bc 0,15±0,00 Ab 0,09±0,00 Ba 0,18±0,00 Bc Ripe 0,14±0,00 Ac 0,14±0,00 Ac 0,18±0,00 Ad 0,15±0,00 Ac 0,06±0,00 Aa 0,095±0,00 Ab Na (μ mol/g DW) Unripe 4,56±0,05 Ca 7,27±0,02 Cd 5,44±0,00 Cb 4,55±0,03 Ca 6,24±0,03 Cc 7,18±0,03 Bd Mid-ripe 4,01±0,04 Bb 6,67±0,03 Bd 5,00±0,87 Bc 3,56±0,04 Ba 5,29±0,08 Bc 7

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Gastroprotective effects of methanol extract of Eremomastax speciosa leaf harvested in Southern part of Nigeria in rat

GC-MS analysis of ESE. The GC-MS chromatogram shown 6 peaks ( Fig. 1 ). The identified compounds are benzenesulfonyl chloride (40.63%), benzopyran-4-one, 5,7-dihydroxy-2-phenyl- (21.25%), benzenesulfonic acid, methyl ester (15.63%), 2(5H)-furanone, 4-methoxy-5-phenyl- (12.50%), 4,5-dimethylthiazole (4.38%) and p-chlorobenzenesulfonyl chloride (5.63%) ( Table 1 ). Table 1 Chemical composition of ESE as identified by the GC-MS S/N RT (min) % MW (g) MF Chemical name 1 30.60 40.63 176 C 6 H 5 ClO 2 S Benzenesulfonyl chloride 2

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Application of whey retentate as complex nitrogen source for growth of the polyhydroxyalkanoate producer Hydrogenophaga pseudoflava strain DSM1023

provided by the Italian dairy company Latterie Vincentine S.c.a., Italy (LAVI). Separation of whey into whey permeate and whey retentate was accomplished directly at LAVI via ultrafiltration. Lactose in whey permeate was hydrolyzed enzymatically by adding the enzyme formulation Maxilact LG 2000 TM (DSM Food Specialities, UK; 2.5 mL per L whey permeate) at a pH of 6.5-7 and a temperature of 37ºC and stirring for 24 hours according to a previously published protocol ( 35 ). The whey retentate fraction was subjected towards acidic hydrolysis by adding 6 M HCl (1 mL per g

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Anti-inflammatory and analgesic activities of methanol extract of Helianthus annuus Linn. (Asteraceae) leaf

.02*(52.27) 0.21 ± 0.01*(36.36) HAE 150 mg/kg 0.36 ± 0.03 (7.69) 0.29 ± 0.04*(34.09) 0.25 ± 0.03*(24.24) HAE 300 mg/kg 0.28 ± 0.03*(28.21) 0.24 ± 0.05*(45.45) 0.22 ± 0.05*(33.33) HAE 600 mg/kg 0.31 ± 0.02*(20.51) 0.24 ± 0.03*(45.45) 0.22 ± 0.03*(33.33) *p < 0.05 compared with 5% Tween-20 treated group; HAE: Helianthus annuus extract, ASA: acetylsalicylic acid Egg albumin-induced paw edema At 3 h post treatment the ASA, HAE 150, 300 and 600 mg/kg caused 35.29%, 29.41%, 32.94% and 31.76% reduction in paw volume in treated

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Fluorescence lifetime imaging of red yeast Cystofilobasidium capitatum during growth

capitatum (CCY-10-1-2) was cultivated in 2l bioreactor Biostat® B plus (Sartorius) on glucose medium (60 g/l). Two-step inoculation was performed in Erlenmeyer flasks in the optimal inoculation medium (in g/l: glucose 40.0, (NH 4 )- 2 SO 4 ) 5.0, KH 2 PO 4 5, MgSO 4 ·7H 2 O 0.696, yeast extract 7.0). The first inoculum (50 ml) was cultivated for 24 h at 28 °C under continuous lighting and shaking. Inoculum I was then transferred into 0.25 l of fresh inoculum II, which was grown under the same conditions as inoculum I. After 24 h, inoculum II was transferred into a

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Artificial cell microcapsules containing live bacterial cells and activated charcoal for managing renal failure creatinine: preparation and in-vitro analysis

were maintained in Lactobacilli MRS Broth (Difco, USA) overnight at 37 ˚C in anaerobic conditions. L. acidophilus was cultivated in 1.5 ml MRS broth, with urea levels starting from 50 mg/Dl, and increasing in increments of 0.3 g/Dl to 2.7 g/Dl. The urea solution was filtered through 0.22 μm filter and added to autoclaved MRS broth. At each urea increment, the bacterium was cultivated for several passages for adaptation, before screening for healthy colonies on modified MRS agar plates enriched with a 0.3 g/dl higher urea concentration. The colonies were then re

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