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Nations. Vitamin and mineral requirements in human nutrition. 2nd edition. Geneva: World Health Organization and Food and Agriculture Organization of the United Nations, 2004. 5. Harrison FE, May JM. Vitamin C function in the brain: vital role of the ascorbate transporter SVCT2. Free Radic Biol Med. 2009 Mar 15;46(6):719-730. 6. Levine M, Padayatty SJ, Espey MG. Vitamin C: a concentration-function approach yields pharmacology and therapeutic discoveries. Adv Nutr. 2011 Mar;2(2):78-88. 7. Kallner A, Hartmann D, Hornig D. Steady-state turnover and body pool of ascorbic

References Adenkola A.Y., Ayo J.O., Sackey A.K.B., Adelaiye A.B. (2009). Haematological and serum biochemical changes in pig administered with ascorbic acid and transported by road for four hours during the harmattan season. J. Cell Anim. Biol., 3: 21–28. Asml A., Agazzi A., Invernizzi G., Bontempo V., Savoini G. (2015). The beneficial role of probiotics in monogastrics animal nutrition and health. J. Dairy Vet. Anim. Res., 2: 116–132. Carr A.C., Maggini S. (2017). Vitamin C and immune function. Nutrients, 9: 1211–1220. Casey P.G., Gardiner G.E., Casey G

References Abalea V, Cillard J, Dubos MP, Sergent O, Cillard P, Morel I. (1999). Repair of iron induced DNA oxidation by the flavonoid myricetin in primary rat hepatocyte cultures. Free Rad Biol Med 26(11-12): 1457-1466. Antunes LM, Takahashi CS. (1998). Eff ects of high doses of vitamins C and E against doxorubicin-induced chromosomal damage in Wistar rat bone marrow cells. Mutat Res 419: 137-143. Benoni G, Cuzzolin L, Zambreri D, Donini M, Del Soldato P, Caramazza I. (1993). Gastrointestinal eff ects of single and repeated doses of ferrous sulphate in rats

References 3. Rahimi R, Nikfar S, Larijani B, Abdollahi M. A review on the role of antioxidants in the management of diabetes and its complication. Biomed Pharmacother 59: 365-373, 2005. 4. Dakhale GN, Cahudhari HV, Shrivastava M. Supplementation of vitamin C reduces blood glucose and improves Glycosylated haemoglobin in type 2 diabetes mellitus: A randomized, double blind study. Advances in Pharmacological Sciences Article ID 195271: 1-5, 2011. 5. Bhatt JK, Thomas S, Nanjan MJ. Effect of oral supplementation of vitamin C on glycemic control and lipid profile in

nanoparticle to carry vitamin C through the gastrointestinal tract and induce the non-specific immunity system of rainbow trout ( Oncorhynchus mykiss ). Carbohydrate Polymers, 86, 1, 142-146. Alishahi, A., Mirvaghefi, A., Tehrani, M., Farahmand, H., Shojaosadati, S., Dorkoosh, F., Elsabee, M. (2011b): Shelf life and delivery enhancement of vitamin C using chitosan nanoparticles. Food Chemistry, 126, 3, 935-940. Baltusnikas, J., Venckunas, T., Kilikevicius, A., Fokin, A., Ratkevicius, A. (2015): Efflux of Creatine kinase from isolated soleus muscle depends on age, sex and

of trehalose in Ascaris suum tissues. Journal of Helminthology 83: 237-243. DOI: 10.1017/S0022149X08165178 Draczyński Z. (2008) Honey bee corpses as an available source of chitin. Journal of Applied Polymer Science 109(3): 1974-1981. DOI: 10.1002/app.28356 Farjan M., Dmitryjuk M., Lipiński Z., Łopieńska-Biernat E., Żółtowska K. (2012) Supplementation of the honey bee diet with vitamin C: The effect on the antioxidative system of Apis mellifera carnica brood at different stages. Journal of Apicultural Research 51(3): 263-270. DOI: 10.3896/IBRA. Farjan M


Nine selected varieties of tomatoes were grown in field experiments in order to follow up changes in the content of vitamin C, depending on the degree of the fruit ripeness. Vitamin C content of green fruit ranged from 6.74 mg (Denár) to 10.23 mg (Salus). The mean value for the varieties in the green state was 8.66 mg.100 g−1. The value of vitamin C in the semi-mature tomatoes ranged from 11.34 mg (Denár) to 14.95 mg (Darinka). The mean value for all varieties was 12.89 mg. 100 g−1. The lowest content of vitamin C was found in consumer (red) ripening tomato varieties Šejk (16.03 mg), Denár (16.32 mg) and Zámčan (16.80 mg) and the highest content of vitamin C in varieties Salus (19.43 mg) and Darinka (19.26 mg). The mean value for the nine varieties was 17.70 mg.100 g−1. In the botanical (overripe) maturity, we recorded the highest vitamin C content in the variety Salus (21.51 mg. 100 g−1). The highest increase in vitamin C was also recorded at the variety Darinka, where we registered the content of 21.32 mg.100 g−1. The lowest vitamin C content in botanical ripeness was observed in the variety Zámčan (19.44 mg.100 g−1). The average amount of vitamin C marked 20.26 g mg.100−1. The results can be concluded that the level of vitamin C is increasing by the gradual ripening of the fruits.

References Boudrant, J. (1990). Microbial processes for ascorbic acid biosynthesis: a review. Enzyme Microb. Technol. 12, 322 - 329. Davies, M. B., Austin, J. & Partridge, D. (1991). Vitamin C: Its chemistry and biochemistry. Cambridge, UK: The Royal Society of Chemistry. Reichstein, T. & Grussner, A. (1934). A good synthesis of L-ascorbic acid (Vitamin C). Helv. Chim. Acta 17, 311 - 328. Šnajdman, L. O. (1973). Proizvodstvo vitaminov. Moskva, Russia: Piščevaja promyšlennost. Paul, E. L., Tung, H. H. & Midler M. (2005). Organic crystallization processes

, Piotrkowski B, Stella I, Fraga CG, et al. Renal mitochondrial impairment is attenuated by AT1 blockade in experimental Type I diabetes. Am J Physiol Heart Circ Physiol 2008; 294: H456-65. 7. Przedborski S, Jackson-Lewis V, Fahn S. Antiparkinsonian therapies and brain mitochondrial complex I activity. Mov Disord. 1995; 10:312-7. 10.1002/mds.870100314 8. Rosa EF, Ribeiro RF, Pereira FMT, Freym­ller E, Aboulafia J, Nouailhetas VLAS. Vitamin C and E supplementation prevents mitochondrial damage of ileum myocytes caused by intense and exhaustive exercise training. J Appl Physiol

mice. Neuroendocrinology 74, 143–147, 2001. Ballaz SJ, Rebec GV. Neurobiology of vitamin C: Expanding the focus from antioxidant to endogenous neuromodulator. Pharmacol Res 146, 104321, 2019. Beheshti F, Karimi S, Vafaee F, Shafei MN, Sadeghnia HR, Hadjzadeh MAR, Hosseini M. The effects of vitamin C on hypothyroidism-associated learning and memory impairment in juvenile rats. Metab Brain Dis 32, 703–715, 2017. Bisbal C, Lambert K, Avignon A. Antioxidants and glucose metabolism disorders. Curr Opin Clin Nutr Metab Care 13, 439–446, 2010. Chebani Y, Marion C, Zizzari P