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References 1. Bujan L., Walschaerts M., Moinard N., Hennebicq S., Saias J., Brugnon F., Auger J., Berthaut I., Szerman E., Daudin M., Rives N.: Impact of chemotherapy and radiotherapy for testicular germ cell tumors on spermatogenesis and sperm DNA: a multicenter prospective study from the CECOS network. Fertil Steril 2013, doi:10.1016/j.fertnstert.2013.05.018. 2. Ceccarelli C., Battisti P., Gasperi M., Fantuzzi E., Pacini F., Gualdrini G., Pierantoni M.C., Luciani A., Djokich D., Pinchera A.: Radiation dose to the testes after 131I therapy for ablation of post

ratio between strains in the scrotal wall of the near-field test area (Z1) and testicular tissue (Z2); ELX-T% HRD is the hardness percentage of strained tissues in the defined area of a testicle with area expressed in mm 2 ; ELX-T% SFT is the percentage measurement of strained tissue softness in parenchyma with the area expressed in mm 2 ; and ElaXto Histogram is the mean value of elasticity of strained points of parenchyma with the area expressed in mm 2 (values derived from the computer-generated histogram of points distribution with different elasticities). The

References Hájek, J., Šidák, Z. & Sen, P. K. (1999). Theory of Rank Tests. Academic Press, San Diego. Hollander, M. & Wolfe, D. A. (1999). Nonparametric Statistical Methods. Wiley, New York. Hutchison, T. P. (2002). Should we routinely test for simultaneous location and scale changes? Ergonomics 45, 248-251. Lepage, Y. (1971). A combination of Wilcoxon's and Ansari-Bradley's statistics. Biometrika 58, 213-217. Lepage, Y. (1973). A table for a combined Wilcoxon Ansari-Bradley statistic. Biometrika 60, 113-116. Podgor, M. J. & Gastwirth, J. L. (1994). On

ABSTRACT

There is good evidence for impairment of spermatogenesis and reductions in sperm counts and testosterone levels in chronic alcoholics. The mechanisms for these effects have not yet been studied in detail. The consequences of chronic alcohol consumption on the structure and/or metabolism of testis cell macromolecules require to be intensively investigated. The present work reports the effects of chronic alcoholism on contents of free amino acids, levels of cytochrome P450 3A2 (CYP3A2) mRNA expression and DNA fragmentation, as well as on contents of different cholesterol fractions and protein thiol groups in rat testes. Wistar albino male rats were divided into two groups: I - control (intact animals), II - chronic alcoholism (15% ethanol self-administration during 150 days). Following 150 days of alcohol consumption, testicular free amino acid content was found to be significantly changed as compared with control. The most profound changes were registered for contents of lysine (-53%) and methionine (+133%). The intensity of DNA fragmentation in alcohol-treated rat testes was considerably increased, on the contrary CYP3A2 mRNA expression in testis cells was inhibited, testicular contents of total and etherified cholesterol increased by 25% and 45% respectively, and protein SH-groups decreased by 13%. Multidirectional changes of the activities of testicular dehydrogenases were detected. We thus obtained complex assessment of chronic alcoholism effects in male gonads, affecting especially amino acid, protein, ATP and NADPH metabolism. Our results demonstrated profound changes in testes on the level of proteome and genome. We suggest that the revealed metabolic disorders can have negative implication on cellular regulation of spermatogenesis under long-term ethanol exposure.

. Gen. Comp. Endocr., 60: 427-433. Denk A.G., Kempenaers B. (2006). Testosterone and testes size in mallards (Anas platyrhynchos). J. Ornithol., 147: 436-440. Forkman B., Corr S. (1996). Influence of size and asymmetry of sexual characters in the rooster and hen on the number of eggs laid. Appl. Anim. Behav. Sci., 49: 285-291. Garamszegi L.Z., Eens M., Hurtrez- Bousses S., Moller A.P. (2005). Testosterone, testes size, and mating success in birds:acomparative study. Horm. Behav., 47: 389-409. Johnsen T.S., Zuk M. (1996). Repeatability of mate choice in female red

References Jiali, H., Zheng, G. (2002). The research of relay protection of three-terminal power transmission lines using optical fiber channel. In PowerCon 2002 : International Conference on Power System Technology , 13-17 October 2002. IEEE, Vol. 2, 696-701. Carpenter, M., Hoad, R.R., Bruton, T.D., Das, R., Kunsman, S.A., Peterson, J.M. (2005). Staged-fault testing for high impedance fault data collection. In Protective Relay Engineers : 58th Annual Conference , 5-7 April 2005. IEEE, 9-17. Xia, Y.Q., David, A.K., Li, K.K. (1994). High-resistance faults on a

References IEEE Standards (2000). IEEE Standard for Terminology and Test Methods for Analog-to-Digital Converters. IEEE Std 1241-2000. IEEE Standards (2007). IEEE Standard for Digitizing Waveform Recorders. IEEE Std 1957-2007. (Revision of IEEE Std 1057-1994). Blair, J. J. (2005). Selecting test frequencies for sinewave tests of ADCs. IEEE Transactions On Instrumentation And Measurement , 54 (1), 73-78. Carbone, P., Petri, D. (2000). Effective frequency-domain ADC testing. IEEE Transactions On Circuits and Systems , 47 (7), 660-663.

Curcuma Longa: Staining Effect on Histomorphology of the Testis

Background: The use of non-allergic, non-toxic and eco-friendly natural dyes has become a matter of significant importance due to the increased environmental awareness in order to avoid some hazardous synthetic dyes.

Method: The ethanolic extract of Curcuma longa was diluted using 1% acetic acid in 70% ethanol to a concentration of 0.2 g/ml. It was used to stain histological sections of the testes for 15 minutes. Curcuma longa was also used as a counter stain for Haematoxylin. Phytochemical constituents were investigated.

Results: The Curcuma longa dye distinctly stained the seminiferous epithelium and interstitium yellow. Curcuma longa provided a good counter stain for Haematoxylin, taking up the acidic staining characteristics with Haematoxylin staining the basic staining characteristics. Phytochemical screening revealed the presence of saponins, alkaloids, tannins and flavonoids.

Conclusion: Curcuma longa has good potential for use as a counter stain for Haematoxylin in the staining of tissues in lieu of Eosin.

) BIOTECHNOLOGICAL METHODS TO INCREASE LANDFILL GAS PRODUCTION AND DEGRADATION OF ORGANIC WASTE. PROC, 3RD HPM WORKSHOP, BRAUNSCHWEIG, 11TH-12TH MARCH 2009. SEITE 13, 1-7. [4] KAVAZANJIAN E., MATASOVIC N. & BACHUS R. (1999) LARGE-DIAMETER STATIC AND DYNAMIC TESTING OF MUNICIPAL SOLID WASTE. PROCEEDINGS SARDINIA ’99, SEVENTH INTERNATIONAL WASTE MANAGEMENT AND LANDFILL SYMPOSIUM, VOL 3, 437-445. [5] BJERRUM, L. (1967). ENGINEERING GEOLOGY OF NORMALLY CONSOLIDATED MARINE CLAYS AS RELATED TO SETTLEMENTS OF BUILDINGS. SEVENTH RANKINE LECTURE. GEOTECHNIQUE, 16(2): 83-118. [6] LANDVA A

References [1] Palček, P., Porubčan, J., Blažek, D., Trojanová, Z. (2007). Internal friction in commercial aluminium alloy AW-2007. Procedia Engineering, 10, 1226-1231. [2] Miller, W.S., Zhuang, L., Bottema, J., Wittebrood, A.J., De Smet, P., Haszler, A. (2000). Recent development in aluminum alloys for the automotive industry. Materials Science and Engineering A, 280, 37-49. [3] Huang, J.C., Shin, C.S., Chan, S.L.I. (2004). Effect of temper, specimen orientation and test temperature on tensile and fatigue properties of wrought and PM AA6061-alloys. International