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M, Devleeschouwer M. Bacterial contamination of indoor air, surfaces, and settled dust, and related dust endotoxin concentrations in healthy office buildings. Ann Agric Environ Med 2005;12:187-92. Hines CJ, Waters MA, Larsson L, Petersen MR, Saraf A, Milton DK. Characterization of endotoxin and 3-hydroxy fatty acid levels in air and settled dust from commercial aircraft cabins. Indoor Air 2003;13:166-73. Park JH, Spiegelman DL, Gold DR, Burge HA, Milton DK. Predictors of airborne endotoxin in the home. Environ Health Perspect 2001;109:859-64.

Der p 1 is the main allergen of house dust mite Dermatophagoides pteronyssinus, which has routinely been detected in residential dust. However, the procedure for extracting Der p 1 from reservoir dust has not been well defi ned. The aim of this study was to compare Der p 1 mass fractions in dust extracts prepared using the following extraction buffers: phosphate (pH 7.4), borate (pH 8.0), and ammonium bicarbonate (pH 8.0), all with 0.05 % Tween 20. Twenty-eight dust samples were divided into three aliquots and each portion was extracted with one of the three buffers at room temperature. Der p 1 mass fractions were measured in a total of 84 dust extracts using the enzyme immunoassay (range: 0.1 μg g-1 to 7.53 μg g-1). Statistical methods including intraclass correlation showed a high agreement between Der p 1 mass fractions irrespective of the extracting medium. Our results suggest that all three buffers are suitable for the extraction of mite allergens and routine Der p 1 analysis in dust.

z drevotrieskových dosák na lineárnu rýchlosť šírenia plameňa (Impact of height, moisture and leaning of the settled dust layer…). In: Wood and fire safety . Zvolen: Technical University in Zvolen, 2004. ISBN 80-228-1321-4 12. SLOSIARIK, J. 2005. Testing of the flame spread linear speed on foodstuffs and agriculture dust layers. In: Komunikácie: vedecké listy Žilinskej university , 2005, Vol. 7, No. 3/2005, pp. 57-59. ISSN 1335-4205

compensated men with silicosis in New South Wales, Australia. Occup Med 2004;54:387-94. 14. Centers for Disease Control and Prevention (CDC). NIOSH Workplace Safety and Health Topics, Silica [displayed 13 July 2011]. Available at 15. Talvitie NA. Determination of free silica: gravimetric and spectrophotometric procedure applicable to air-borne and settled dust. Am Ind Hyg Assoc J 1964;25:169- 78. 16. Federal Register. US code of Federal Regulations 29CFR-1910.1200. Hazard communication. 1 st ed. Washington (DC). Federal Register

, regulations and administrative provisions of the Member States relating to restrictions on the marketing and use of certain dangerous substances and preparations (phthalates in toys and childcare articles). Off J Eur Commun 2005;L 344:40-3. 15. Leibowitz JN, Sarimento R, Sugar SM, Ethridge MW. Determination of six common phthalate plasticizers in grain neutral spirits and vodka. J AOAC Int 1995;78:730-5. 16. Kolarik B, Bornehag C, Naydenov K, Sundell J, Stavova P, Nielsen O. The concentration of phthalates in settled dust in Bulgarian homes in relation to building

and Air-O-Cell data in relation to physical assessments of mold damage and some other parameters. Indoor Air 2005;15:257-66. Douwes J, Siebers R, Wouters IM, Doekes G, Fitzharris P, Crane J. Endotoxin, (1→3)-β-D-glucans and fungal extra-cellular polysaccharides in New Zealand homes: a pilot study. Ann Agric Environ Med 2006;13:361-5. Salare VR, Hinde CA, Miller JD. Analysis of settled dust in homes and fungal glucan in air particulate collected during HEPA vacuuming. Indoor Built Environ 2009;18:485-91. Park SJ, Mehrad B. Innate immunity to Aspergillus species