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Abstract

In this study, selected components of seminal plasma in equine semen were evaluated. Levels of enzymes, electrolytes, microelements and some other components were observed. The aim of this study was to find some important differences between the levels of these components and the total sperm motility after freezing and thawing (freezability of the semen). Total of 32 ejaculates from 7 stallions were collected, assessed and prepared in 0,5 ml straws for freezing. After thawing, the sperm motility was analyzed and ejaculates were divided into two groups: “good” freezable and “poor” freezable. The only statistically significant difference between groups of „good“ and „poor“ freezable ejaculates was in the concentration of vitamin E in the seminal plasma. In the group of „good“ freezable ejaculates, the level of vitamin E was significantly lower (p≤0,05) than in the group of “poor” freezable ejaculates

Abstract

This study is aimed at investigating effects of supplementation of stallion’ semen extender with various concentrations of Gum Arabic (GA) versus egg yolk (EY) on viscosity, sperm motility and survival during cooling and freezing. Physical sperm characteristics; i.e. curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN) and straightness index (STR) were evaluated. Based on the sperm velocity (velocity of the average path), individual spermatozoons were classified into two major groups; i.e., progressively motile (>45 μm/sec) and immotile (0-45 μm/sec) spermatozoa. Addition of 3, 9 or 15% of GA to HF-20 extender resulted in linear decreases in VCL, VSL and VAP and a decrease in the percentage of progressively motile spermatozoa. Dilution of horse semen samples with high viscosityextenders (i.e., high percentage of GA) decreased the VCL, VSL and VAP in fresh and chilled semen. Freezing semen in high viscosity-extenders reduced percentage of progressively motile spermatozoa compared with those of low viscosity-extenders. In refrigerated and frozen semen samples, the extender containing 15% GA had detrimental effects on the percentage of progressively motile sperm cells and velocity of progressive motile sperm. Moreover, cooling sperm in extenders containing 9 or 15% of GA for 72 hours resulted in complete motility cessation. In conclusion, GA could replace EY in stallion semen extenders at a level of 3% to maintain the physical and biological characteristics of cold and frozen semen.

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