In this study, selected components of seminal plasma in equine semen were evaluated. Levels of enzymes, electrolytes, microelements and some other components were observed. The aim of this study was to find some important differences between the levels of these components and the total sperm motility after freezing and thawing (freezability of the semen). Total of 32 ejaculates from 7 stallions were collected, assessed and prepared in 0,5 ml straws for freezing. After thawing, the sperm motility was analyzed and ejaculates were divided into two groups: “good” freezable and “poor” freezable. The only statistically significant difference between groups of „good“ and „poor“ freezable ejaculates was in the concentration of vitamin E in the seminal plasma. In the group of „good“ freezable ejaculates, the level of vitamin E was significantly lower (p≤0,05) than in the group of “poor” freezable ejaculates
This study is aimed at investigating effects of supplementation of stallion’ semen extender with various concentrations of Gum Arabic (GA) versus egg yolk (EY) on viscosity, sperm motility and survival during cooling and freezing. Physical sperm characteristics; i.e. curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), linearity (LIN) and straightness index (STR) were evaluated. Based on the sperm velocity (velocity of the average path), individual spermatozoons were classified into two major groups; i.e., progressively motile (>45 μm/sec) and immotile (0-45 μm/sec) spermatozoa. Addition of 3, 9 or 15% of GA to HF-20 extender resulted in linear decreases in VCL, VSL and VAP and a decrease in the percentage of progressively motile spermatozoa. Dilution of horse semen samples with high viscosityextenders (i.e., high percentage of GA) decreased the VCL, VSL and VAP in fresh and chilled semen. Freezing semen in high viscosity-extenders reduced percentage of progressively motile spermatozoa compared with those of low viscosity-extenders. In refrigerated and frozen semen samples, the extender containing 15% GA had detrimental effects on the percentage of progressively motile sperm cells and velocity of progressive motile sperm. Moreover, cooling sperm in extenders containing 9 or 15% of GA for 72 hours resulted in complete motility cessation. In conclusion, GA could replace EY in stallion semen extenders at a level of 3% to maintain the physical and biological characteristics of cold and frozen semen.
REFERENCES Al Ahmad MZA, Chatagnon G, Amirat-Briand L, Moussa M, Tainturier D, Anton M, Fieni F (2008): Use of glutamine and low density lipoproteins isolated from egg yolk to improve buck semenfreezing. Reproduction in Domestic Animals, 43, 429–436. doi: 10.1111/j.1439-0531.2008.01270.x. Bencharif D, Amirat-Briand L, Garand A, Anton M, Schmitt E, Desherces S, Delhomme G, Langlois M-L, Barrière P, Destrumelle S, Vera-Munoz O, Tainturier D (2010): Freezing canine sperm: Comparison of semen extenders containing Equex and LDL (Low Density Lipoproteins). Animal
.D., Zhao H.: 8-hydroxy-2′-deoxyguanosine as a potential survival biomarker in patients with non-small cell lung cancer. Cancer 2007, 109, 574–580. Shen J. Deininger P. Hunt J.D. Zhao H. 8-hydroxy-2′-deoxyguanosine as a potential survival biomarker in patients with non-small cell lung cancer Cancer 2007 109 574 – 580 32 Silva A.R., de Cássia Soares Cardoso R., Uchoa D.C., Machado da Silva L.D.: Effect of tris-buffer, egg yolk, and glycerol on canine semenfreezing. Vet J 2002, 164, 244–246. Silva A.R. de Cássia Soares Cardoso R. Uchoa D.C. Machado da Silva L.D. Effect of
future importance to the industry. Theriogenology 70: 1251-1259. 19. Großfeld, R., Sieg, B., Struckmann, C., Franzel, A., Maxwell, W.M.C., Rath, D. (2008). New aspects of boar semenfreezing strategies. Theriogenology 70: 1225-1233. 20. Knox, R.V. (2011). The current value of frozenthawed boar semen for commercial companies. Reprod Domest Anim 46 (Suppl. 2): 4-6. 21. Khalifa, T.A.A., Tsakmakidis, I.A. (2012). A preliminary study on preservability of porcine semen in INRA-96 extender. Proceeding of the 17th International Congress on Biotechnology in Animal Reproduction
. Iranian Journal of Basic Medical Sciences, 16 (6), 731-742. Fakhrildin, M. B. & Alsaadi, R. A. R. (2014). Honey Supplementation to Semen-Freezing Medium ImprovesHuman Sperm Parameters Post-Thawing. Journal of Family & Reproductive Health, 8 (1), 27-31. Farouk, A., Hassan, T., Kashif, H., Khalid, S. A., Mutawali, I., Wadi, M. (1988). Studies on Sudanese Bee Honey: Laboratory and Clinical Evaluation. International Journal of Crude Drug Research, 26 (3), 161-168. DOI: 10.3109/13880208809053912 Fauzi, A. N., Norazmi, M. N., & Yaacob, N. S. (2011). Tualang honey induces