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wild ruminants in Italy using ribosomal DNA marker. Int. J. Parasitol., 29: 1809–1817. DOI: 10.1016/S0020- 7519(99)00123-X [14] Gibson, T. E., Everett, G. (1981): The ecology of the free living stages of Nematodirus battus. Res. Vet. Sci., 31: 323–327 [15] González, B. A., Palma, R. E., Zapata, B., Marín, J. C. (2006): Taxonomic and biogeographical status of guanaco Lama guanicoe (Artiodactyla, Camelidae). Mammal. Rev. 36(2): 157–178. DOI: 10.4067/S0716-078X2007000200001


Longidorus caespiticola was found for the first time from the rhizosphere of apple with two other Longidorus sp. at Žlunice, Czech Republic. Females and males were analyzed morphologically and morphometrically. Four regions (18S, ITS1, ITS2, D2-D3 expansion segments of 28S) of ribosomal DNA and one region (cox1) of mitochondrial DNA were sequenced and analyzed and compared with a population from the Slovak Republic. The partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene showed relatively high genetic variation compared with ribosomal DNA between the Czech and Slovakian populations.


Soil samples under the rhizosphere of Brasicca napus were collected from three localities (Bílé Podolí, Prague, Kylešovice). Two localities Prague and Kylešovice were positive for the presence of Pratylenchus neglectus. The species was identified using morphological features and the morphological identification was verified by using published species-specific primers and by sequencing 18S and 28S genes of ribosomal DNA.

. Parasitol., 85: 431–435 [9] Hoste, H., Gasser, R. B., Chilton, N. B., Mallet, S., Beveridge, I. (1993): Lack of intraspecific variation in the second internal transcribed spacer (ITS-2) of Trichostrongylus colubriformis rDNA. Int. J. Parasitol., 23: 1069–1071 [10] Hoste, H., Chilton, N. B., Gasser, R. B., Beveridge, I. (1995): Differences in the second internal transcribed spacer (ribosomal DNA) between five species of Trichostrongylus (Nematoda: Trichostrongylidae). Int. J. Parasitol., 25

D. S. S. Ribosomal DNA and Phylogeny of the Ascaridoidea Nemata: Secernentea): Implications for Morphological Evolution and Classification // Molecular Phylogenetics and Evolution. — 1998. — 10 (2). — P. 221-236. Nadler S. A., Hudspeth D. S. S. Phylogeny of the Ascaridoidea (Nematoda: Ascaridida) based on three genes and morphology: hypothesis of structural and sequence evolution // J. of Parasitology. — 2000. — 86 (2). — P. 380-393. Nadler S. A., Carreno R. A., Mejia-Madrid H. et al. Molecular phylogeny of clade III nematodes reveals multiple origins of

Ribosomal DNA. J. Nematol .. 40: 201 - 209 19440260 Bae CH Szalanski AL Robbins RT 2008 Molecular Analysis of the Lance Nematode, Hoplolaimus spp., Using the First Internal Transcribed Spacer and the D1 – D3 Expansion Segments of 28S Ribosomal DNA J. Nematol 40 201 209 Baylis, H.A. (1930): A third species of the nematode genus Thubunaea. Ann. mag. nat. hist .. 5: 246 - 249. DOI: 10.1080/00222933008673128 10.1080/00222933008673128 Baylis HA 1930 A third species of the nematode genus Thubunaea Ann. mag. nat. hist 5 246 249 10.1080/00222933008673128 Baylis, H.A. (1936

most supraspecific taxa of calcareous sponges (Porifera, Calcarea) revealed by increased taxon sampling and partitioned Bayesian analysis of ribosomal DNA. Molecular Phylogenetics and Evolution, 40: 830-843. ERPENBECK D., WÖRHEIDE G. 2007: On the molecular phylogeny of sponges (Porifera) In: Zhang Z-Q, Shear WA, editors. Linnaeus Tercentenary: Progress in Invertebrate Taxonomy. Zootaxa 1668. Auckland (New Zealand): Magnolia Press, 107-126. FEDOR M.J., WILLIAMSON J.R. 2005: The catalytic diversity of RNAs. Nature Reviews Molecular Cell Biology, 6: 399-412. JAEGER J


Soil samples under the rhizosphere of Brasicca napus were collected from three localities (Bílé Podolí, Prague, Kylešovice). All three localities were positive for the presence of Aphelenchus avenae. Morphological and molecular features of A. avenae are presented in this research note.


The distribution of Pratylenchus species associated with wheat crops was investigated in Bohemian region of the Czech Republic. In total twelve localities were sampled. The populations were identified based on morphology and morphometrics, and further characterised based on sequences of the rDNA D2/D3 region and 18S gene. Pratylenchus crenatus was present in two localities and P. neglectus in five localities. At one locality both species were detected. Sequence analysis of 18S and D2/D3 region of three populations of P. crenatus reveal no variation while five populations of P. neglectus differ by 0 to 0.14 % (18S) and 0.17 to 0.50 % (D2/D3).


Morphological Redescription and Molecular Characterization of Dactylogyrus labei (Monogenea, Dactylogyridae) from Catla catla: a New Host Record in India. Chiary, H. R., Chaudhary, A., Singh, H. S. - Dactylogyrus labei Musselius et Gusev, 1976 (atypical form) specimens were collected from host Catla catla from the river Brahmaputra, Guwahati, Assam, India. The Dactylogyrus samples were examined by morphological and molecular methods. This parasite was originally described from Labeo rohita from fish farm Kalyani, West Bengal, India, but the morphological study of sclerotized parts showed the existence of D. labei on Catla catla. This is the first record of D. labei from Catla catla in India.