Martin Nikolovski, Ljupco Mickov, Monika Dovenska, Vladimir Petkov, Branko Atanasov and Toni Dovenski
, F., Leonard, T., Hartman, T.D., North, R.D., Moore, H.D. (1989). The value of sperm swimming speed measurements in assessing the fertility of human frozen semen. Hum. Reprod. 4, 292-297. PMid:2715304
31. Anel-Lopez, L., Alvarez-Rodriguez, M., Garcia- Alvarez, O., Alvarez, M., Maroto-Morales, A., Anel, L., de Paz, P., Garde, J.J., Martinez-Pastor, F. (2012). Reducedglutathione and Trolox (vitamin E) as extender supplements in cryopreservation of red deer epididymal spermatozoa. Anim. Reprod. Sci., 135; (1-4): 37-46. http://dx.doi.org/10.1016/j
Esmat A. Hassan, Ahmed M.S. Hussein and Azza A.A. Hussein
This study aimed to produce a baked dietary product (pate) enriched with broccoli florets that were sprayed in the field with methionine. Treated broccoli florets were separately stuffed in pate at three levels (5, 10 and 15%, w:w) in the form of minced, steam blanched and fried broccoli florets. A significant increase in reduced glutathione content was observed in the methionine-treated broccoli florets compared to methionine-untreated broccoli florets during processing to minced, steam blanched or as fried with butter. The pate stuffed with methionine-treated broccoli florets at different levels had higher fiber and protein contents if compared to control sample (not stuffed pate). Stuffing the processed pate had no effect on the estimated rheological properties, color attributes, baking tests and organoleptic properties. Increasing the ratio of stuffed methionine-treated broccoli florets increased loaf weight and decreased crumb moisture. The results revealed that stuffing pate with methionine-treated broccoli florets had enriched the nutritive value and baking quality. Generally, pate stuffed with methionine-treated broccoli florets (5:15%) did not significantly affect technological, rheological, sensory quality of pate and improved its nutritional values.
Martin Nikolovski, Monika Dovenska, Ksenija Ilievska, Nikola Adamov, Branko Atanasov, Miroslav Radeski, Daniela Kirovski, Vladimir Petkov and Toni Dovenski
Reduced glutathione (GSH) and homologous ram seminal plasma (HSP), used as additives in cryopreserving (CP) media prior to freezing, showed conflicting results in retaining structural integrity and progressive motility in post-thawed ram spermatozoa. The aims of this research were: (1) to assess the effect of GSH and/or HSP supplementation via soybean-lecithin CP extender on cryopreserved ram spermatozoa viability, morphology and motility pattern; and (2) to assess the effect of incubation in the context of the previous aim. Quantitatively and qualitatively, homogenized and pooled ram ejaculates (N=10) were extended with one of the following extenders: control (C) – tris-based, GSH and HSP-free, experimental-1 (E1) – C + GSH 5 mM, experimental-2 (E2) – C + HSP 20 % and experimental-3 (E3) - GSH 5 mM + HSP 20 %. Following thawing, samples were taken at 0- and 3-hours from each group (n=10) and were assessed for spermatozoa viability, morphology, and motility pattern. C-0h samples yielded a spermatozoa population with low viability, altered head morphology and highly deviated motility pattern. E3-3h samples yielded spermatozoa with unaffected viability, head morphology and high progressive motility. In conclusion, E3 extender added to cryopreserved-thawed ram spermatozoa is most efficient in obtaining high viability, unaltered head morphology, and progressive motility.
Sterodin®, a novel immunostimulating drug: Some toxicological and pharmacological evaluations in vivo, and drug-lipid interaction studies in vitro
Sterodin® is a novel non-specific immunostimulating drug produced by a combination of bile lipids and bacterial metabolites. In the present study, we investigated some of its (i) toxicological and (ii) pharmacological properties in vivo, and (iii) drug-lipid interaction (lipid peroxidation) in vitro. We also evaluated the possible (iv) Sterodin®-induced lipid peroxidation as well as the effect of ascorbic acid on this peroxidation. We found LD50 of Sterodin® to be 31.50 mL kg-1 body mass. In male albino mice, Sterodin® increased the total white blood cells and neutrophils count by 59 and 26%, respectively, on the 6th day, compared to day 0 after injection and stimulated phagocytic activity in vivo. We used goat liver as lipid source in drug-lipid interaction studies in vitro. Our experiments show that Sterodin® induces lipid peroxidation, which was prevented by ascorbic acid.
Tania Shamim Rizvi, Abdul Latif Khan, Liaqat Ali, Narjis Al-Mawali, Fazal Mabood, Javid Hussain, Muhammad Adnan and Ahmed Al-Harrasi
10. J. Hussain, L. Ali, A. L. Khan, N. Rehman, F. Jabeen, J. S. Kim and A. Al-Harrasi, Isolation and bioactivities of the flavonoids morin and morin-3- O -β-D-glucopyranoside from Acridocarpus orientalis – A wild Arabian medicinal plant, Molecules 19 (2014) 17763–17772; https://doi.org/10.3390/molecules191117763
11. E. U. Ezeji, E. A. Anyalogbu, T. N. Ezejiofor and J. U. Udensi, Determination of reducedglutathione and glutathione S-transferase of poultry birds exposed to permethrin insecticide, Am. J. Biochem. 2 (2012) 21
Dusica Djordjevic, Jelica Stojanovic Tosic, Djordje Stefanovic, Nevena Barudzic, Milena Vuletic, Vladimir Zivkovic and Vladimir Jakovljevic
: Beutler E, ed. Red cell metabolism, a manual of biochemical methods. New York: Grune and Stratton, 1982: 105-6.
15. Tsuchihashi M. Zur Kernntnis der blutkatalase. Biochem Z 1923; 140: 65-72.
16. Misra HP, Fridovich I. The role of superoxide-anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972; 247(10): 3170-5.
17. Beutler E. Reducedglutathione (GSH). In: Beutler E, ed. Red cell metabolism, a manual of biochemical methods. New York: Grune and Stratton, 1975: 112-4.
18. Radovanovic D, Jakovljevic
Deniel Pesic, Ivan Srejovic, Djordje Stefanovic, Dusica Djordjevic, Dejan Cubrilo and Vladimir Zivkovic
of epinephrine and a simple assay for superoxide dismutase. J Biol Chem. 1972; 247(10): 3170-5.
18. Beutler E. Reducedglutathione - GSH. In: Beutler E (ed) Red cell metabolism: a manual of biochemical methods. Grane and Straton, New York. 1975. 112-114.
19. Kratz A, Lewandrowski KB, Siegel AJ, Chun KY, Flood JG, Van Cott EM, Lee-Lewandrowski E. Effect of marathon running on hematologic and biochemical laboratory parameters, including cardiac markers. Am J Clin Pathol. 2002; 118(6): 856-63.
20. Traiperm N, Gatterer H
Aleksandra Vranic, Aleksandra Antovic, Nevena Draginic, Marijana Andjic, Marko Ravic, Vladimir Jakovljevic and Mirjana Veselinovic
22. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxidedismutase. J BiolChem 1972; 247: 3170-5.
23. Beutler E. Reducedglutathione (GSH). In: Beutler E (ed) Red cell metabolism, a manual of biochemical methods. Grune and Stratton, New York 1975, pp 112-4.
24. Bauerová K, Bezek A. Role of reactive oxygen and nitrogen species in etiopathogenesis of rheumatoid arthritis. Gen Physiol Biophys 1999; 18 Spec No: 15-20. DOI: 10.1007/978-3-642-18619-6.
, erythrocytes, and plasma were separated and stored at −20°C until genotyping and biochemical measurements were conducted.
Determination of reducedglutathione using Ellman’s reagent
Whole blood (0.1 mL) was added to distilled water (1.9 mL) together with 3 mL of precipitating solution (100 mL containing 1.67 g glacial metaphosphoric acid, 0.2 g disodium EDTA, and 30 g sodium chloride). After standing for 5 min the mixture was filtered and filtrate (0.5 mL) was added to 0.3 M phosphate buffer, pH 6.4 (2 mL). Finally, we added 1 mM 5,5′-dithiobis-(2-nitrobenzoic acid