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Health Care 2006; 15: 102–8. 10. Pang PS, Cleland JG, Teerlink JR, Collins SP, Lindsell CJ, Sopko G, et al. A proposal to standardize dyspnoea measurement in clinical trials of acute heart failure syndromes: the need for a uniform approach. Eur Heart J 2008; 29: 816–24. 11. Salvagno GL, Sanchis-Gomar F, Picanza A, Lippi G. Red blood cell distribution width: A simple parameter with multiple clinical applications. Crit Rev Clin Lab Sci 2015; 52: 86–105. 12. Turcato G, Serafini V, Dilda A, Bovo C, Caruso B, Ricci G, et al. Red blood cell distribution width independently

G, Montagnana M. Red blood cell distribution width and cardiovascular diseases. J Thorac Dis 2015; 7:E402-11. 23. Montagnana M, Danese E. Red cell distribution width and cancer. Ann Transl Med. 2016; 4(20):399. DOI: 10.21037/atm.2016.10.50 24. Guglielmi V, Manchisi M, Pellegrini V, Tutino M, Guerra V. RDW: new screening test for coeliac disease? Minerva Med. 2002; 93(5):419-421. 25. Martha O, Porav-Hodade D, Bălan D, Tătaru OS, Sin A, Chibelean CB, et al. Easily Available Blood Test Neutrophil- To-Lymphocyte Ratio Predicts Progression in High-Risk Non

, Montagnana M. Red blood cell distribution width and cardiovascular diseases. J Thorac Dis 2015; 7: E402-11. 14. Allen LA, Felker GM, Mehra MR, Chiong JR, Dunlap SH, Ghali JK, et al. Validation and potential mechanisms of red cell distribution width as a prognostic marker in heart failure. J Card Fail 2010; 16: 230-8. 15. Imai R, Uemura Y, Okumura T, Takemoto K, Uchikawa T, Koyasu M, et al. Impact of red blood cell distribution width on non-cardiac mortality in patients with acute decompensated heart failure with preserved ejection fraction. J Cardiol 2017; 70: 591-7. 16

. Notably, some demographic (i.e., age, sex), laboratory (i.e., C reactive protein, lipoprotein[a], homocysteine), lesion and procedural risk factors have been identified and used during the last decades, but none of these was found to have an optimal diagnostic efficiency for predicting short- and long-term risk of PTA-related complications or reocclusion in large prospective studies ( 5 ). On the other hand, Lee et al recently showed that in-hospital increase of red blood cell distribution width (RDW) value, which reliably mirrors an escalating impairment of erythrocyte

References 1. van Kimmenade R, Mohammed A, Uthamalingam S, van der Meer P, Felker G, Januzzi J. Red blood cell distribution width and 1-year mortality in acute heart failure. European Journal of Heart Failure. 2010;12(2):129-136. 2. Ani C, Ovbiagele B. Elevated red blood cell distribution width predicts mortality in persons with known stroke. Journal of the Neurological Sciences. 2009;277(1-2):103-108. 3. Perlstein T, Weuve J, Pfeffer M, Beckman J. Red Blood Cell Distribution Width and Mortality Risk in a Community-Based Prospective Cohort. Archives of Internal

References 1. Karnad A, Poskitt TR. The automated complete blood count. Use of the red blood cell volume distribution width and mean platelet volume in evaluating anemia and thrombocytopenia. Arch Intern Med 1985; 145: 1270-2. 2. Bessman JD, Gilmer PR Jr, Gardner FH. Improved classification of anemias by MCV and RDW. Am J Clin Pathol 1983; 80: 322-6. 3. Tsuboi S, Miyauchi K, Kasai T, Ogita M, Dohi T, Miyazaki T, et al. Impact of red blood cell distribution width on long-term mortality in diabetic patients after percu - taneous coronary ıntervention. Circ J 2013

platelet volume and their relation to prognosis in cerebral infarction. J. Intern. Med. 1990;227(1):11-4. 29. Scharte M, Fink MP. Red blood cell physiology in critical illness. Crit Care Med. 2003;31(12):S651-7. 30. Fukuta H, Ohte N, Mukai S, et al. Elevated plasma levels of B-type natriuretic peptide but not C-reactive protein are associated with higher red cell distribution width in patients with coronary artery disease. Int Heart J. 2009;50(3):301-12. 31. Mahmood NA, Mathew J, Kang B, DeBari VA, Khan MA. Broadening of the red blood cell distribution width is

;11:2253-8. 15. Demircan F, Gözel N, Kılınç F, Ulu R, Atmaca M. The Impact of Red Blood Cell Distribution Width and Neutrophil/Lymphocyte Ratio on the Diagnosis of Major Depressive Disorder. Neurol Ther. 2016;5(1):27-33. 16. Balta S, Demırkol S, Kucuk U. The platelet lymphocyte ratio may be useful inflammatory indicator in clinical practice. Hemodial Int. 2013;17(4):668-9. 17. Mazza MG, Lucchi S, Tringali AGM, Rossetti A, Botti ER, Clerici M. Neutrophil/lymphocyte ratio and platelet/lymphocyte ratio in mood disorders: A meta-analysis. Prog Neuropsychopharmacol Biol Psychiatry

Abstract

Ketosis of lactating cows is among the most common metabolic diseases in modern dairy farms. The economic importance of the disease is caused by the reduced milk yield and body weight loss, poor feed conversion, lower conception rates, culling and increased mortality of affected animals. In the present study, a total of 47 high-yielding dairy cows up to 45 days in milk (DIM) are included. All animals were submitted to physical examination wich included checking the rectal body temperature, heart rate, respiratory and rumen contraction rates, and inspection of visible mucous coats. The body condition was scored, and blood β-hydroxybutyrate (BHBA) concentrations were assayed. The cows were divided into 3 groups: first group (control) (n=24) with blood β-hydroxybutyrate level <1.2 mmol/l, second group (n=15) with blood β-hydroxybutyrate between 1.2-2.6 mmol/l (subclinical ketosis) and third group (n=8) with blood β-hydroxybutyrate >2.6 mmol/l (clinical ketosis). Whole blood samples were obtained and analyzed for Red Blood Cell (RBC, 1012/l), Hemoglobin (HGB, g/l), Hematocrit (HCT, %), Mean Corpuscular Volume (MCV, fl), Mean Corpuscular Hemoglobin (MCH, pg), Mean Corpuscular Hemoglobin Concentration (MCHC, g/l), White Blood Cell (WBC, 109/l), Lymphocytes (LYM, 109/l), Monocytes (MON, 109/l), Granulocytes (GRA, 109/l), Red Blood Distribution Width (RDW, %), Red Blood Cell Distribution Width Absolute (RDWa, fl), Platelets (PLT, 109/l) and Mean Platelet Volume (MPV, fl). In this study, deviations in the clinical parameters in the control group and in those with subclinical ketosis were not identified. The cows from the third group (clinical ketosis) exhibited hypotonia, anorexia and body weight loss vs. control group. Hematological analysis showed leukocytosis and lymphocytosis in cows with subclinical ketosis vs. control group. In cows with clinical ketosis WBC counts decreased (leukopenia), while hemoglobin content and hematocrit values are higher vs. control group. Blood BHBA values are higher in both groups of ketotic cows vs. the control group. The other analyzed parameters (RBC, MCH, MCHC, MCV, RDW, RDWa, MON, GRA, PLT and MPV) were close to control values.

; 107: 3841-6. 5. Mekky M, Jasuja M, Parkin PC. Moderate and severe microcytic anemia in the emergency department: indicators of care. Clin Pediatr (Phila) 2009; 48: 902-3. 6. Kristinsson G, Shtivelman S, Hom J, Tunik MG. Pre va - lence of occult anemia in an urban pediatric emergency department: what is our response? Pediatr Emerg Care 2012; 28: 313-15. 7. Montagnana M, Cervellin G, Meschi T, Lippi G. The role of red blood cell distribution width in cardiovascular and thrombotic disorders. Clin Chem Lab Med 2011; 50: 635-41. 8. Ershler WB, Chen K, Reyes EB, Dubois R