Search Results

1 - 10 of 328 items :

  • "real-time PCR" x
Clear All
Original Article. Evaluation of Rapid Detection of Nasopharyngeal Colonization with MRSA by Real-Time PCR

patients admitted to critical care. J Hosp Infect 2007;65:24-28. 14 Bootsma MC, Diekmann O, Bonten MJ. Controlling methicillinresistant Staphylococcus aureus: quantifying the effects of interventions and rapid diagnostic testing. Proc Nat Acad Sci USA 2006;103:5620-5625. 15 Rossney AS, Herra CM, Morgan PM, Morgan PM, O’Connell B. Evaluation of the Xpert methicillin-resistant Staphylococcus aureus (MRSA) assay using the genexpert Real-Time PCR platform for rapid detection of mrsa from screening specimens. J Clin Microbil 2008

Open access
Simultaneous Quantification and Genotyping of Hepatitis C Virus RNA by a Two-Step Real-Time PCR Assay on the Lightcycler Instrument

real-time PCR by use of the MagNA Pure LC and LightCycler Instruments. Clin Microbiol 2004;42:585-90. Abe M, Klett C, Wieland E. Gille S, Landt O. Two-step real-time PCR quantification of all subtypes of human immunodeficiency virus type 1 by an in-house method using locked nucleic acid-based probes. Folia Medica 2008;50(3):5-13. Chen Z, Weck KE. Hepatitis C virus genotyping: interrogation of the 5' untranslated region cannot accurately distinguish genotypes 1a and 1b. Clin Microbiol 2002

Open access
Development of Simple Multiplex Real-Time PCR Assays for Foodborne Pathogens Detection and Identification On Lightcycler

concentration of food-borne pathogens in food samples prior to quantification by viable-cell counting and real-time PCR. Appl Environ Microbiol, 73(1): 92-100. PMid:17056684 PMCid:PMC1797114 6. Fukushima, H., Kawase, J., Etoh, Y., Sugama, K., Yashiro, S., Iida, N., Yamaguchi, K. (2010). Simultaneous screening of 24 target genes of foodborne pathogens in 35 foodborne outbreaks using multiplex real-time SYBR green PCR analysis. International Journal of Microbiology 12010, Article ID 864817, 18 pages.

Open access
Evaluation of real-time PCR based on SYBR Green I fluorescent dye for detection of Bacillus anthracis strains in biological samples

.C.B.: Anthrax in animals. Mol Aspects Med 2009, 30, 481–489. 4. Bode E., Hurtle W., Norwood D.: Real-time PCR assay for a unique chromosomal sequence of Bacillus anthracis . J Clin Microbiol 2004, 42, 5825–5831. 5. Budniak S., Kędrak-Jabłonska A., Reksa M., Szczawińska A., Borkowska-Opacka B.: Application of the real-time PCR method with the intercalating dye SYBR Green I for the detection of genes located on plasmids pXO1 and pXO2 as well as the specific chromosomal rpoB sequence of Bacillus anthracis strains. Med Weter 2010, 66, 630–634. 6. D’Amelio E

Open access
Evaluation of Slovak Wheat Cultivars for Fusarium Culmorum Infection by Real-Time PCR and By Conventional Assays

tissues using real_time PCR in comparison with DON content. In Journal Phytopathology , vol. 154 , 2006, pp. 603-611. McCARTNEY, H. A. - FOSTER, S. J. - FRAAIJE, B. A. - WARD, E. 2003. Molecular diagnostics for fungal plant pathogens. In Pest Management Science , vol. 59 , 2003, no. 5, pp. 129-142. DOI: 10.1002/ps.575. MORADI, M. - OERKE, E. C. - STEINER, U. - TESFAYE, D. - SCHELLANDER, K. - DEHNE, H. W. 2010. Microbiological and Sybr (R) Green real-time PCR detection of major Fusarium head blight pathogens on

Open access
Detection of Listeria spp. and Listeria monocytogenes in biological samples by SYBR Green I and TaqMan probe-based real-time PCRs

References 1. Amagliani G., Omiccioli E., Brandi G., Bruce I.J., Magnani M.: A multiplex magnetic capture hybridisation and multiplex real-time PCR protocol for pathogen detection in sea food. Food Microbiol 2010, 27, 580–585. 2. Barkallah M., Gharbi Y., Hmani M., Mallek Z., Gautier M., Gdoura R., Fendri I.: Locked nucleic acid probe-based real-time PCR for the diagnosis of Listeria monocytogenes in ruminants. Mol Cell Probe 2016, 30, 138–145. 3. Budniak S., Kędrak-Jabłońska A., Szczawińska A., Reksa M., Krupa M., Szulowski K.: Comparison of

Open access
Non-Invasive Fetal Sex Determination Using SRY Specific Primers and Sybrgreen Real Time PCR

.W., Leung T.N., Chan L.Y., et al. Fetal clearance from maternal plasma is impaired in preeclampsia. Clin. Chem. 2002; 48: 2141-2146. Hromadnikova I., Holubova B., Hridelova D. et al. Replicate real - time PCR testing of DNA in maternal plasma increases the sensitivity of non - invasive fetal sex determination. Prenat diagn 2003, 23: 235-238 Page-Christiaens GC, Bossers B, van der Schoot CE, de Haas M. Use of bi-allelic insertion/deletion polymorphisms as a positive control for fetal genotyping in maternal blood: first

Open access
Application of SYBR Green I and TaqMan probe-based real-time PCRs for the identification of Listeria spp. and Listeria monocytogenes

References 1. Barbau-Piednoir E., Botteldoorn N., Yde M., Mahillon J., Roosens N.H.: Development and validation of qualitative SYBR®Green Real-Time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes. Appl Microbiol Biotechnol 2013, 97, 4021-4037. 2. Bassler H.A., Flood S.J.A., Livak K.J., Marmaro J., Knorr R., Batt C.A.: Use of a fluorogenic probe in a PCR-based assay for the detection of Listeria monocytogenes. Appl Environ Microbiol 1995, 61, 3724-3728. 3. Dmowska K., Wieczorek K

Open access
Real-Time PCR Assays for the Specific Detection of Field Balkan Strains of Lumpy Skin Disease Virus

goatpox viruses. Journal of Virology 2002, 76:6054-6061. 10. Heine HG. Stevens MP. Foord A.J. Boyle DB: A capripoxvirus detection PCR and antibody ELISA based on the major antigen P32, the homolog of the vaccinia virus H3L gene. Journal of Immunological Methods 1999. 227:187-196. 11. Haegeman A, Zro K, Vandenbussche F, Demeestere L, Van Campe W, Ennaji MM, De Clercq K: Development and validation of three Capripoxvirus real-time PCRs for parallel testing. J Virol Methods 2013, 193(2):446-51. 12. Hosamani M, Mondal B

Open access
The efficiency of sodC gene / N. meningitidis detection in comparison with the classical methods for the diagnosis of meningococcal infection / Evaluarea eficienţei Real Time PCR TaqMan utilizând gena sodC / N. meningitidis în comparaţie cu metodele clasice utilizate în diagnosticul infecţiei meningococice

reaction-based detection of Neisseria meningitidis in sterile body fluids. MemInst Oswaldo Cruz. 2013 Apr;108(2):246-7. DOI: 10.1590/0074-0276108022013020 4. Dolan Thomas J, Hatcher CP, Satterfield DA, Theodore MJ, Bach MC, Linscott KB, et al. Sod C-based real-time PCR for detection of Neisseria meningitidis. PLoS One. 2011 May 5;6(5):e19361. DOI: 10.1371/ journal.pone.0019361 5. Abdeldaim GM, Strålin K, Korsgaard J, Blomberg J, Welinder-Olsson C, Herrmann B. Multiplex quantitative PCR for detection of lower respiratory tract infection and

Open access