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Pollen Transcriptome and Proteome: Molecular and Functional Analysis

] HEIDSTRA R, WELCH D, SCHERES B. Mosaic analyses using marked activation and deletion clones dissect Arabidopsis SCARECROW action in asymmetric cell division. Genes Dev 2004; 18: 1964-1969 [33] HEPLER PK, VIDALI L, CHEUNG AY. Polarized cell growth in higher plants. Annu Rev Cell Dev Biol 2001; 17: 159-187. [34] HOLMES-DAVIS R, TANAKA CK, VENSEL WH, HURKMAN WJ, McCORMICK S. Proteome mapping of mature pollen of Arabidopsis thaliana. Proteomics 2005; 5: 4864-4884. [35] HONYS D, COMBE JP, TWELL D, ČAPKOVÁ V. The translationally

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Clinical Proteomics in Kidney Disease: From Discovery to Clinical Application

diseases: unraveling the pathophysiology and biomarker discovery. Expert Rev Proteomics. 2005; 2(3): 349-366. 5. Andersen S, Mischak H, Zürbig P et al. Urinary proteome analysis enables assessment of renoprotective treatment in type 2 diabetic patients with microalbuminuria. BMC Nephrol. 2010; 11(1): 29- 6. Stalmach A, Albalat A, Mullen W, and Mischak H. Recent advances in capillary electrophoresis coupled to mass spectrometry for clinical proteomic applications. Electrophoresis. 2013; 34(11): 1452-1464. 7. Pontillo C, Filip S, Borras DM et al. CE

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Increased protein excretion, including albumin, by children of patients with urolithiasis

children, to validate these abnormalities for risk assessment, and to investigate further the urinary proteome and pathophysiology of urolithiasis. Materials and methods The present study, including assent forms for vulnerable participants (those aged 7-12 years, and 13-18 years old) was approved by Institutional Review Board of the Department for Development of Thai Traditional and Alternative Medicine as part of Ministry of Public Health, Thailand (approval No. RLC0029/55), and IRB of Sunpasit Prasong Hospital. We enrolled 116 participants in this study into 4

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Urinary Proteome Analysis using Capillary Electrophoresis Coupled to Mass Spectrometry: A Powerful Tool in Clinical Diagnosis, Prognosis and Therapy Evaluation

standards. Proteomics Clin Appl 2007; 1: 148-56. Coon JJ, Zürbig P, Dakna M, Dominiczak AF, Decramer S, Fliser D, et al. CE-MS analysis of the human urinary proteome for biomarker discovery and disease diagnostics. Proteomics Clin Appl 2008; 2: 964-73. Castronovo V, Kischel P, Guillonneau F, De LL, Defechereux T, De PE, et al. Identification of specific reachable molecular targets in human breast cancer using a versatile ex vivo proteomic method. Proteomics 2007; 7: 1188-96. Roessler M

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Application of Preparative Electrophoresis for Clinical Proteomics in Urine: Is it Feasible?

References Thongboonkerd V. Recent progress in urinary proteomics. Proteomics Clin Appl 2007; 1: 780-91. Fliser D, Novak J, Thongboonkerd V, Argilés A, Jankowski V, Girolami MA, Jankowski J, Mischak H. Advances in urinary proteome analysis and biomarker discovery. J Am Soc Nephrol 2007; 18 (4): 1057-71. Decramer S, Gonzalez de Peredo A, Breuil B, Mischak H, Monsarrat B, Bascands JL, Schanstra JP. Urine in clinical proteomics. Mol Cell Proteomics 2008; 7 (10): 1850

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Proteomics of the Spermatozoon

-791. 20. Pixton KL, Deeks ED, Flesch FM, et al. Sperm proteome mapping of a patient who experienced failed fertilization at IVF reveals altered expression of at least 20 proteins compared with fertile donors: case report. Hum Reprod. 2004; 19(6): 1438-1447.

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Human Seminal Plasma Proteome Study: a Search for Male Infertility Biomarkers

ABSTRACT

Seminal plasma is a potential source of biomarkers for many disorders of the male reproductive system including male infertility. Knowledge of the peptide and protein components of seminal fluid is accumulating especially with the appearance of highthroughput MS-based techniques. Of special interest in the field of male infertility biomarkers, is the identification and characterization of differentially expressed proteins in seminal plasma of men with normal and impaired spermatogenesis. However, the data obtained until now is still quite heterogeneous and with small percentage of overlap between independent studies. Extensive comparative analysis of seminal plasma proteome is still needed in order to establish a potential link between seminal plasma proteins and male infertility.

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Proteomics of heavy metal toxicity in plants

Proteome Res 2009;8:400-17. doi: 10.1021/pr800561r 27. Giordano PM, Mortvedt J, Mays A. Effect of municipal wastes on crop yields and uptake of heavy metals. J Environ Q u a l 1 9 7 5 ; 4 : 3 9 4 - 9 . d o i : 1 0 . 2 1 3 4 / jeq1975.00472425000400030024x 28. Islam E, Yang X, He Z, Mahmood Q. Assessing potential dietary toxicity of heavy metals in selected vegetables and food crops. J Zhejiang Univ Sci B 2007;8:1-13. doi: 10.1631/ jzus.2007.B0001 29. Fu J, Zhou Q, Liu J, Liu W, Wang T, Zhang Q, Jiang G. High levels of heavy

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Proteomic Insight Into the Molecular Principles of Grapevine Habituation

Proteomic Insight Into the Molecular Principles of Grapevine Habituation

Two-dimensional gel electrophoresis coupled to protein microarray analysis was used to examine, for the first time, the molecular mechanisms of grapevine (Vitis vinifera L., cv. Limberger) habituation. The examination of 2-D maps derived from control and habituated cell culture revealed the presence of 55 protein spots displaying a differential expression pattern. These facts have provide a molecular evidence suggesting that the habituated cells can be used as a model for study of cell differentiation and plant defense mechanisms. Cell death, extra-cellular alkalinization and expression of genes responsible for the formation of the defense-related proteins were analyzed in suspension cultures with hormonal autonomy (habituation). Results obtained using habituated grapevine cells compared with non-habituated cells were different and strongly depended on the concentration of elicitor applied.

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Snake Venom Metalloproteinases

using a venom gland transcriptomics and proteomics approach. J Proteome Res. 2010;9(4):1882-1893. 26. Weldon CL, Mackessy SP. Biological and proteomic analysis of venom from the Puerto Rican Racer (Alsophis Portoricensis: Dipsadidae). Toxicon. 2010;55:558-569. 27. Georgieva D, Seifert J, Betzel C et al. Pseudechis australis venomics: adaptation for a defense against microbial pathogens and recruitment of body transferrin. 2011;10(5):2440-2464. 28. Petras D, Sanz L, Calvette JJ et al. Snake venomics of African spitting

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